Navigation Links
loxP flanked Chloramphenicol Selection Cassette (loxP-cm-loxP) from Gene Bridges GmbH

ProductsloxP flanked Chloramphenicol Selection Cassette (loxP-cm-loxP) from Gene Bridges GmbH
Company Gene Bridges GmbH
Item loxP flanked Chloramphenicol Selection Cassette (loxP-cm-loxP)
Price 
Description The loxP-cm-loxP cassette is designed to allow chloramphenicol selection in prokaryotic cells.
The prokaryotic promoter gb2 driving the gene for chloramphenicol resistance is a slightly modified version of the Em7 promoter. It mediates higher transcription efficiency than the generally used Tn5 promoter. A synthetic polyadenylation signal terminates the chloramphenicol expression. The cassette is flanked by loxP sites for later excision by Cre recombinase. Unique NotI and XhoI sites flank the cassette for convenient cloning with restriction sites.
Using the provided PCR template one can easily create a loxP-cm-loxP cassette flanked by any other restriction sites to clone the cassette into the vector of choice. The restriction sites can be introduced by adding the corresponding sequence in the PCR primer. The template can easily be used to engineer the E.coli genome by Red/ET Recombination.
The loxP-cm-loxP cassette is not linear but plasmid based (3301 bp in size). Due to its R6K origin the plasmid cannot replicate in most E.coli strains. The PCR product can therefore be used directly for downstream applications without any further purification.
Info Gene Bridges GmbH
Commercial Centre
Im Neuenheimer Feld 584
69120 Heidekberg, Germany
Customer Service: +49 (0)6221 13708 10
Fax Number: +49 (0)6221 13708 29
Web Site: http://www.Genebridges.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. FRT flanked, Pro and Euk. Neomycin Selection Cassette (FRT-PGK-gb2-neo-FRT) from Gene Bridges GmbH
2. loxP flanked, Pro and Euk. Neomycin Selection Cassette (loxP-PGK-gb2-neo-loxP) from Gene Bridges GmbH
3. FRT flanked, Pro and Euk. Neomycin Selection Cassette plus loxP site(FRT-PGK-gb2-neo-FRT-loxP) from Gene Bridges GmbH
4. FRT flanked Ampicillin Selection Cassette (FRT-amp-FRT) from Gene Bridges GmbH
5. loxP flanked Ampicillin Selection Cassette (loxP-amp-loxP) from Gene Bridges GmbH
6. Mouse Anti-Chloramphenicol Acetyltransferase Monoclonal Antibody, Unconjugated from Novus Biologicals
7. Mouse Anti-Chloramphenicol Acetyltransferase Monoclonal Antibody, Unconjugated, Clone CAT-1 from Abcam
8. Chloramphenicol Acetyltransferase Fluorometric (TMR) Assay Kit, FAST CAT from Molecular Probes (Invitrogen)
9. Chloramphenicol Acetyltransferase Fluorometric (BODIPY) Assay Kit, FAST CAT from Molecular Probes (Invitrogen)
10. EasySep Biotin Selection Kit, Other from StemCell Technologies, Inc.
11. EasySep PE Selection Kit, Other from StemCell Technologies, Inc.
...
... Western Blot Strong Antibody Stripping Solution is ... blots that have been developed with chemiluminescence ... is not recommended for stripping colorimetric substrates ... not possible to effectively remove substrates that ...
... Liquid. 100 ml supplied as a ... remove antibodies from membrane-bound proteins without ... stripping chemiluminescent or radioisotopic signals from ... Western blots. Supplied as a 5X ...
... is specifically designed for two-dimensional electrophoresis ... four proteins in this marker have ... pattern across a 2D gel. The ... Mol wt: 17,000-89,000 ...
Biology Products:
(Date:7/30/2014)... changing climate threaten some of the world,s most ... establish seed collections to save the seeds in ... some genetic diversity., For decades, these seed collections ... a one-size-fits-all approach for how many seeds to ... regardless of species, pollination mode, growth habitat and ...
(Date:7/30/2014)... they eat, the Food and Drug Administration (FDA) earlier ... label found on nearly every food product in the ... (C&EN), the weekly news magazine of the American ... fight that has ensued. , Britt Erickson, a senior ... Facts label has remained largely the same for two ...
(Date:7/30/2014)... from Wayne State University,s College of Engineering recently ... Foundation for the project, "Nanoparticle-directed synthesis of organic ... Foundation, nanotechnology is the creation and utilization of ... and functions. A major bottleneck in scaling up ... connect different functional materials into one device. A ...
Breaking Biology News(10 mins):Saving seeds the right way can save the world's plants 2NSF grant to Wayne State supports new concept for manufacturing nanoscale devices 2
... amorous midshipman fish to the strains of a Puccini aria ... neural circuitry that led to the human love song not ... of vertebrates was likely laid down hundreds of millions of ... By mapping the developing brain cells in newly hatched midshipman fish ...
... for food service and salad mixes is an increasingly ... perishable, and the cutting required in processing further shortens ... fresh produce in semipermeable plastic films extends shelf life ... modified-atmosphere (MA) packaging for lettuce and certain salad greens ...
... joining forces to conceptualize, develop and bring to reality ... care through UC San Diego,s new Institute of Engineering ... to repair damaged hearts, and scarless surgery techniques are ... with the new Institute leading the way. "As ...
Cached Biology News:From humming fish to Puccini: Vocal communication evolved with ancient species 2Good breeding increases shelf life 2UC San Diego launches Institute of Engineering in Medicine to accelerate health care tech 2UC San Diego launches Institute of Engineering in Medicine to accelerate health care tech 3
(Date:7/30/2014)... 2014  Orexigen Therapeutics, Inc. (Nasdaq: OREX ... List of Outstanding Issues (LOI) from the European Medicines ... for the NB32 Marketing Authorization Application (MAA). NB32 (naltrexone ... drug candidate being evaluated for weight loss. ... point were adequately addressed by the Company based in ...
(Date:7/30/2014)... July 30, 2014 Researchers often use ... biology, but such devices can do much more ... As such, researchers have looked to patterned assemblies ... metabolic engineering to characterize – and potentially ... devices are envisioned to recreate animal and human ...
(Date:7/30/2014)... 2014  AtheroNova Inc. (OTCBB: AHRO), a biotech ... compounds to safely regress atherosclerotic plaque and improve ... partner, CardioNova, has accomplished first dosing of subjects ... lead compound, AHRO-001.  This Phase 1b trial will ... safety trial completed in February 2014, in which ...
(Date:7/30/2014)... (PRWEB) July 30, 2014 NCERC ... Athmanathan will present his findings on corn stover ... conference this week in Washington, D.C. , ... Energy conference is a testament to the success ... Caupert said. “Thanks to the foresight and vision ...
Breaking Biology Technology:Orexigen Receives CHMP Day 180 List of Outstanding Issues 2Orexigen Receives CHMP Day 180 List of Outstanding Issues 3UMD Researchers Bridge Gap between Microelectronics, Biological Systems 2UMD Researchers Bridge Gap between Microelectronics, Biological Systems 3UMD Researchers Bridge Gap between Microelectronics, Biological Systems 4AtheroNova Announces Milestone with Initiation of Phase 1b Clinical Trial of AHRO-001 2AtheroNova Announces Milestone with Initiation of Phase 1b Clinical Trial of AHRO-001 3AtheroNova Announces Milestone with Initiation of Phase 1b Clinical Trial of AHRO-001 4NCERC at SIUE Researcher Takes Stage at Department of Energy Conference 2NCERC at SIUE Researcher Takes Stage at Department of Energy Conference 3