loxP flanked Ampicillin Selection Cassette (loxP-amp-loxP) from Gene Bridges GmbH

Products	loxP flanked Ampicillin Selection Cassette (loxP-amp-loxP) from Gene Bridges GmbH
Company	Gene Bridges GmbH
Item	loxP flanked Ampicillin Selection Cassette (loxP-amp-loxP)
Price
Description	The loxP-amp-loxP cassette is designed to allow ampicillin selection in prokaryotic cells. The prokaryotic promoter gb2 driving the gene for ampicillin resistance is a slightly modified version of the Em7 promoter. It mediates higher transcription efficiency than the generally used Tn5 promoter. A synthetic polyadenylation signal terminates the ampicillin expression. The cassette is flanked by lox sites for later excision by Cre recombinase. Unique NotI and XhoI sites flank the cassette for convenient cloning with restriction sites. Using the provided PCR template one can easily create a loxP-amp-loxP cassette flanked by any other restriction sites to clone the cassette into the vector of choice. The restriction sites can be introduced by adding the corresponding sequence in the PCR primer. The template can easily be used to engineer the E.coli genome by Red/ET Recombination. The loxP-amp-loxP cassette is not linear but plasmid based (3502 bp in size). Due to its R6K origin the plasmid cannot replicate in most E.coli strains. The PCR product can therefore be used directly for downstream applications without any further purification.
Info	Gene Bridges GmbH Commercial Centre Im Neuenheimer Feld 584 69120 Heidekberg, Germany Customer Service: +49 (0)6221 13708 10 Fax Number: +49 (0)6221 13708 29 Web Site: http://www.Genebridges.com

NOTE: Price information is approximate list price and actual prices may vary.

... Safe Deposit Service ECACC is able to accept ... cGMP for cryo-storage. The cell lines must be ... been produced in accordance with the requirements of ... freedom from mycoplasma, bacteria or fungi. What are ...

Standard and modified oligonucleotides up to 150 nt-long at any scale for broad applications. Price starts from 30p/base (10 nmole scale)....

... of expertise in the field of Enzyme-Linked ... most powerful systems on the market. Freedom ... in-microplate tests requiring incubation and washing steps. ... (such as worktable shaker incubators, reader,washer), all ...

... Choice Microsphere/Microarray Stabilizer is a is a ... This product is an aqueous solution ... buffer (phosphate buffered saline), pH 6.6 - ... of 0.02% methylisothiazolone and 0.02% bromonitrodioxane as ...

Biology Products:

cGMP Safe Deposit Facility from European Collection of Cell Cultures (ECACC)Standard and Modified Oligonucleotides Service from Yorkshire Bioscience Ltd ELISA Biopharma from Tecan StabilGuard Choice Microsphere/Microarray Stabilizer from SurModics, Inc.

(Date:5/20/2013)... Boulder, Colo., USA New Geology ... 16 May 2013 cover a wide swath of ... tectonics, oceanography, geophysics, and paleobotany. Locations studied include ... mine at Alpi Apuane, Italy; Ukraine; Mars; and ... , 1. Rubies, jadeite, and plate tectonics;, ...

(Date:5/19/2013)... bacteria that can grow using hydrogen gas as its ... source of carbon. Researchers at the University of ... Meeting of the American Society for Microbiology. , "This ... hydrogen," says Amit Kumar, a researcher on the study ... Lovley Lab Group at the university. , Under the ...

(Date:5/18/2013)... MD (May 19, 2013) The AGA Research Foundation ... and Damian Augustyn Award in Digestive Cancer, which will ... of Medicine at the University of Pennsylvania, Philadelphia, as ... in pancreas development, regeneration and cancer progression. , "The ... in honor of two distinguished clinicians and AGA Legacy ...

[0] New in GEOLOGY: Gems, Darwin, Mars, Hemp, Snowball Earth, a Siberian Impact Crater, and More [0] Bacteria use hydrogen, carbon dioxide to produce electricity [0] AGA Research Foundation grant furthers digestive cancer research

... , Registration is now open for the Ecological Society ... in Portland, Oregon. The meeting is expected to draw more ... share emerging research. Jane Lubchenco, an Oregon State marine ... will open the six day meeting with a keynote address ...

... Combined, complimentary therapies have the ability to maximize ... spinal cord repair in rat models, according to a ... who published in a recent issue of Cell Transplantation ... "When transplanted, neural stem cells have demonstrated their ...

... April 17, 2012 /PRNewswire-iReach/ -- Global Information ... showing strong growth in the medical devices markets, driven ... Renal Dialysis Equipment Market to 2017 ... increasingly large elderly population are driving rapid growth in ...

Cached Biology News:Registration open for the Ecological Society of America's 2012 Annual Meeting in Portland, Ore. 2 Neural stem cell transplants for spinal cord injury maximized by combined, complimentary therapies 2 Global Surgical Devices Market Growth Driven by Improving Standards of Living and Longer Life Expectancies, Research Shows 2 Global Surgical Devices Market Growth Driven by Improving Standards of Living and Longer Life Expectancies, Research Shows 3 Global Surgical Devices Market Growth Driven by Improving Standards of Living and Longer Life Expectancies, Research Shows 4

Registration open for the Ecological Society of America's 2012 Annual Meeting in Portland, Ore.Neural stem cell transplants for spinal cord injury maximized by combined, complimentary therapies Global Surgical Devices Market Growth Driven by Improving Standards of Living and Longer Life Expectancies, Research Shows

(Date:5/20/2013)... CHAPEL HILL, N.C. , May 20, 2013 ... pharmaceutical company focused on developing differentiated antibiotics to ... bacterial infections, today announced that it presented data ... 2013) in Philadelphia demonstrating ... versus levofloxacin in Cempra,s prior Phase 2 clinical ...

(Date:5/20/2013)... WILMINGTON, Del. (PRWEB) May 20, 2013 ... named 14 young faculty members to its 2013 ... three years, the company will provide this outstanding ... their work in advancing basic science to meet ... is designed to help promising young and untenured ...

(Date:5/19/2013)... (PRWEB) May 19, 2013 Switzerland’s ... today announced a new speed record for a transatlantic ... Las Palmas, Spain, on April 25, 2013, the solar ... Ocean at the average speed of 5.3 knots before ... on May 18, 2013. The first-of-its-kind catamaran completed this ...

(Date:5/17/2013)... recipient,s immune system identifies the transplanted organ as foreign ... T cells, the immune cells that mediate rejection, must ... order to migrate to the transplanted organ. In this ... Fadi Lakkis and colleagues at the University of Pittsburgh ... cells is not required for migration. Instead, these cells ...

Breaking Biology Technology:Cempra Presents Post-Phase 2 Analysis of Solithromycin's Efficacy and Safety Results from Patients with Community Acquired Bacterial Pneumonia (CABP) 2 DuPont Celebrates Scientific Innovation by Recognizing Young Professors 2 DuPont Celebrates Scientific Innovation by Recognizing Young Professors 3 MS Tûranor PlanetSolar Sets World Speed Record for Transatlantic Crossing by Solar Electric Vessel 2 MS Tûranor PlanetSolar Sets World Speed Record for Transatlantic Crossing by Solar Electric Vessel 3 MS Tûranor PlanetSolar Sets World Speed Record for Transatlantic Crossing by Solar Electric Vessel 4 MS Tûranor PlanetSolar Sets World Speed Record for Transatlantic Crossing by Solar Electric Vessel 5 MS Tûranor PlanetSolar Sets World Speed Record for Transatlantic Crossing by Solar Electric Vessel 6

[0] Cempra Presents Post-Phase 2 Analysis of Solithromycin's Efficacy and Safety Results from Patients with Community Acquired Bacterial Pneumonia (CABP)[0] DuPont Celebrates Scientific Innovation by Recognizing Young Professors [0] MS Tûranor PlanetSolar Sets World Speed Record for Transatlantic Crossing by Solar Electric Vessel [0] Researchers identify signals that direct the immune system to reject a transplanted organ