Navigation Links
loxP flanked Ampicillin Selection Cassette (loxP-amp-loxP) from Gene Bridges GmbH

ProductsloxP flanked Ampicillin Selection Cassette (loxP-amp-loxP) from Gene Bridges GmbH
Company Gene Bridges GmbH
Item loxP flanked Ampicillin Selection Cassette (loxP-amp-loxP)
Price 
Description The loxP-amp-loxP cassette is designed to allow ampicillin selection in prokaryotic cells.
The prokaryotic promoter gb2 driving the gene for ampicillin resistance is a slightly modified version of the Em7 promoter. It mediates higher transcription efficiency than the generally used Tn5 promoter. A synthetic polyadenylation signal terminates the ampicillin expression. The cassette is flanked by lox sites for later excision by Cre recombinase. Unique NotI and XhoI sites flank the cassette for convenient cloning with restriction sites.
Using the provided PCR template one can easily create a loxP-amp-loxP cassette flanked by any other restriction sites to clone the cassette into the vector of choice. The restriction sites can be introduced by adding the corresponding sequence in the PCR primer. The template can easily be used to engineer the E.coli genome by Red/ET Recombination.
The loxP-amp-loxP cassette is not linear but plasmid based (3502 bp in size). Due to its R6K origin the plasmid cannot replicate in most E.coli strains. The PCR product can therefore be used directly for downstream applications without any further purification.
Info Gene Bridges GmbH
Commercial Centre
Im Neuenheimer Feld 584
69120 Heidekberg, Germany
Customer Service: +49 (0)6221 13708 10
Fax Number: +49 (0)6221 13708 29
Web Site: http://www.Genebridges.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. FRT flanked, Pro and Euk. Neomycin Selection Cassette (FRT-PGK-gb2-neo-FRT) from Gene Bridges GmbH
2. loxP flanked, Pro and Euk. Neomycin Selection Cassette (loxP-PGK-gb2-neo-loxP) from Gene Bridges GmbH
3. FRT flanked, Pro and Euk. Neomycin Selection Cassette plus loxP site(FRT-PGK-gb2-neo-FRT-loxP) from Gene Bridges GmbH
4. loxP flanked Chloramphenicol Selection Cassette (loxP-cm-loxP) from Gene Bridges GmbH
5. FRT flanked Ampicillin Selection Cassette (FRT-amp-FRT) from Gene Bridges GmbH
6. Mouse Anti-Ampicillin Monoclonal Antibody, Unconjugated, Clone 164-12-2 from Abcam
7. EasySep Biotin Selection Kit, Other from StemCell Technologies, Inc.
8. EasySep PE Selection Kit, Other from StemCell Technologies, Inc.
9. EasySep PE Selection Kit, Other, Starter Kit from StemCell Technologies, Inc.
10. EasySep FITC Selection Kit, Other from StemCell Technologies, Inc.
11. FailSafe PCR PreMix Selection Kit from EPICENTRE Biotechnologies
Reacti-Bind DNA Coating Solution...
... regulate cell stretching or compression of a ... Computer controlled so you can precisely regulate ... to produce 64 unique strain parameters. ST-160 ... ST-150. Both models are compatible with Nikon, ...
... EasyCount ViaSure Kit is used in conjunction with ... nucleated cells automatically. The ViaSure Staining Reagent ... - Acridine Orange and Ethidium Bromide to identify ... test, while the EasyCount Slide 6 Multi-well ...
... Basic power supply are used for electrophoretic blotting ... accommodates two 7.5 x 10 cm gels and ... Bio-Ice cooling unit, buffer tank, lid with power ... power supply, 100-120 and 220-240 V, provides output ...
Biology Products:
(Date:7/23/2014)... a thirsty bird happens upon a vessel of water, but ... water level out of his reach. Not strong enough to ... one at a time until the water level ... the value of ingenuity, the fable demonstrates that cognitive ability ... characterizes crows as pretty resourceful problem solvers. New research conducted ...
(Date:7/23/2014)... Mass. Honey bees, especially the young, are highly ... work together to maintain temperatures within a narrow range. ... biologist at Tufts University,s School of Arts and Sciences, ... excess heat within a hive in process similar to ... blood vessels and skin. , "This study shows ...
(Date:7/23/2014)... NEWPORT, Ore. -- A comprehensive 15-year analysis of the ... of the United States found that their favored feeding ... the threat of injury and mortality. , The researchers ... and San Francisco to slightly different areas at ... most abundant could significantly decrease the probability of ...
Breaking Biology News(10 mins):Smarter than a first-grader? 2Smarter than a first-grader? 3Smarter than a first-grader? 4How honey bees stay cool 215-year analysis of blue whale range off California finds conflict with shipping lanes 215-year analysis of blue whale range off California finds conflict with shipping lanes 315-year analysis of blue whale range off California finds conflict with shipping lanes 4
... OTO EXPO of the American Academy of Otolaryngology Head ... nose, and throat doctors in the world, will convene September ... scientific research sessions, 594 posters, and several hundred instruction course ... for journalists from around the world to cover breaking science ...
... 2010 - Eurofins MWG Operon, an expert in next generation ... GS Junior. The GS Junior is the optimum system for ... one eighth of the capacity of a GS FLX Titanium ... run. As a consequence, the GS Junior adds a higher ...
... This release is available in French ... high electrical conductivity that promise to revolutionize a wide range ... A team of McGill University researchers have now reported ... along one single conductive polymer molecule at a time, a ...
Cached Biology News:2010 AAO-HNSF miniseminars: Tuesday, Sept. 28, 2010 22010 AAO-HNSF miniseminars: Tuesday, Sept. 28, 2010 3Striding towards a new dawn for electronics 2
(Date:7/22/2014)... Terry Baughn, a senior lecturer in mechanical engineering at ... Engineer of the Year by the North Texas section ... ASME has more than 130,000 members in 158 countries ... technology to assist the engineering community, benefiting the world. ... Texas Society of Professional Engineers honors banquet. Baughn retired ...
(Date:7/22/2014)... New York (PRWEB) July 22, 2014 ... world. The heat produced by controlled fission chain reaction ... This steam pressure is used to drive a power- ... approximately 19% of the world’s total electricity production. Nuclear ... to several under construction, planned and proposed nuclear reactor ...
(Date:7/22/2014)... 22, 2014 Automated cell culture systems ... of cell culture. They allow production of optimum cells ... researchers in drug discovery and industries and biopharmaceutical companies. ... production, iPSC research, cryobanking, cell-based assays, and cellular expression ... , Analysts forecast the Global Cell Culture market ...
(Date:7/22/2014)... July 22, 2014 The DNA ... and concise visual representation of history, migration, and culture. ... always regaled by family history stories stemming from Europe. ... the "old country." Pictures and 8mm home movies painted ... of the family, and Steve ordered a test from ...
Breaking Biology Technology:UT Dallas professor receives Engineer of the Year award 2Nuclear Energy Market: Global Industry Analysis, Size, Share, Analysis, Growth and Forecast 2013 - 2019 2Nuclear Energy Market: Global Industry Analysis, Size, Share, Analysis, Growth and Forecast 2013 - 2019 3Global Cell Culture Market to Grow at 10.27% CAGR by 2018 Forecasts a New Research Report ReportsnReports.com 2Global Cell Culture Market to Grow at 10.27% CAGR by 2018 Forecasts a New Research Report ReportsnReports.com 3Global Cell Culture Market to Grow at 10.27% CAGR by 2018 Forecasts a New Research Report ReportsnReports.com 4DNA Test from DNA Spectrum Shows Heritage Like a Map of the Past 2DNA Test from DNA Spectrum Shows Heritage Like a Map of the Past 3DNA Test from DNA Spectrum Shows Heritage Like a Map of the Past 4