Navigation Links
loxP flanked Ampicillin Selection Cassette (loxP-amp-loxP) from Gene Bridges GmbH

ProductsloxP flanked Ampicillin Selection Cassette (loxP-amp-loxP) from Gene Bridges GmbH
Company Gene Bridges GmbH
Item loxP flanked Ampicillin Selection Cassette (loxP-amp-loxP)
Price 
Description The loxP-amp-loxP cassette is designed to allow ampicillin selection in prokaryotic cells.
The prokaryotic promoter gb2 driving the gene for ampicillin resistance is a slightly modified version of the Em7 promoter. It mediates higher transcription efficiency than the generally used Tn5 promoter. A synthetic polyadenylation signal terminates the ampicillin expression. The cassette is flanked by lox sites for later excision by Cre recombinase. Unique NotI and XhoI sites flank the cassette for convenient cloning with restriction sites.
Using the provided PCR template one can easily create a loxP-amp-loxP cassette flanked by any other restriction sites to clone the cassette into the vector of choice. The restriction sites can be introduced by adding the corresponding sequence in the PCR primer. The template can easily be used to engineer the E.coli genome by Red/ET Recombination.
The loxP-amp-loxP cassette is not linear but plasmid based (3502 bp in size). Due to its R6K origin the plasmid cannot replicate in most E.coli strains. The PCR product can therefore be used directly for downstream applications without any further purification.
Info Gene Bridges GmbH
Commercial Centre
Im Neuenheimer Feld 584
69120 Heidekberg, Germany
Customer Service: +49 (0)6221 13708 10
Fax Number: +49 (0)6221 13708 29
Web Site: http://www.Genebridges.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. FRT flanked, Pro and Euk. Neomycin Selection Cassette (FRT-PGK-gb2-neo-FRT) from Gene Bridges GmbH
2. loxP flanked, Pro and Euk. Neomycin Selection Cassette (loxP-PGK-gb2-neo-loxP) from Gene Bridges GmbH
3. FRT flanked, Pro and Euk. Neomycin Selection Cassette plus loxP site(FRT-PGK-gb2-neo-FRT-loxP) from Gene Bridges GmbH
4. loxP flanked Chloramphenicol Selection Cassette (loxP-cm-loxP) from Gene Bridges GmbH
5. FRT flanked Ampicillin Selection Cassette (FRT-amp-FRT) from Gene Bridges GmbH
6. Mouse Anti-Ampicillin Monoclonal Antibody, Unconjugated, Clone 164-12-2 from Abcam
7. EasySep Biotin Selection Kit, Other from StemCell Technologies, Inc.
8. EasySep PE Selection Kit, Other from StemCell Technologies, Inc.
9. EasySep PE Selection Kit, Other, Starter Kit from StemCell Technologies, Inc.
10. EasySep FITC Selection Kit, Other from StemCell Technologies, Inc.
11. FailSafe PCR PreMix Selection Kit from EPICENTRE Biotechnologies
... Solid phase reagent ... purification; Easy & unique ... Compatible with detergents and ... & recover non-envelop viruses ...
Tumor Suppressor Sampler Kit 10 mu g each...
Monoclonal Antibody toSynapsin I a and I b Neurofilament associated protein from mouse brain extracts (4)....
Slide Chambers are convenient for performing pre-hybridization incubations and post-hybridization washes with tissue sections mounted on standard glass microscope slides. Each chamber holds up to 5 s...
Biology Products:
(Date:3/30/2017)... -- The research team of The Hong Kong Polytechnic ... by adopting ground breaking 3D fingerprint minutiae recovery and matching technology, ... accuracy for use in identification, crime investigation, immigration control, security of ... ... A research team led by Dr ...
(Date:3/29/2017)... -- higi, the health IT company that operates the largest ... , today announced a Series B investment from BlueCross ... new investment and acquisition accelerates higi,s strategy to create ... health activities through the collection and workflow integration of ... and secures data today on behalf of over 36 ...
(Date:3/27/2017)... ROCKVILLE CENTRE, N.Y. , March 27, 2017 ... by Healthcare Information and Management Systems Society (HIMSS) ... Analytics Outpatient EMR Adoption Model sm . In ... top 12% of U.S. hospitals using an electronic ... recognized CHS for its high level of EMR ...
Breaking Biology News(10 mins):
(Date:5/23/2017)... ... May 22, 2017 , ... NetDimensions has been ranked ... Research Globe™ for Corporate Learning, 2017. , Aragon Research defines Leaders as organizations ... effectively perform against those strategies. NetDimensions’ ranking as a Leader due to its ...
(Date:5/23/2017)... ... 2017 , ... Bacterial biofilms, surface adherent communities of bacteria that are encased ... food poisoning and catheter infections to gum disease and the rejection of medical implants. ... dollars per year, there is currently a paucity of means for preventing their formation ...
(Date:5/22/2017)... , ... May 22, 2017 , ... ... that it is exhibiting in booth B2 at the Association for Pathology ... 22-25. , In addition to demonstrating its Cancer Diagnostic Cockpit and Consultation ...
(Date:5/18/2017)... Mass. (PRWEB) , ... May 18, 2017 , ... ... the procedure on April 28, 2017 at the Prince Of Wales Private Hospital. ... cervical disc at level C6-C7. The patient failed conservative treatments prior to undergoing ...
Breaking Biology Technology: