Navigation Links
loxP flanked, Pro and Euk. Neomycin Selection Cassette (loxP-PGK-gb2-neo-loxP) from Gene Bridges GmbH

ProductsloxP flanked, Pro and Euk. Neomycin Selection Cassette (loxP-PGK-gb2-neo-loxP) from Gene Bridges GmbH
Company Gene Bridges GmbH
Item loxP flanked, Pro and Euk. Neomycin Selection Cassette (loxP-PGK-gb2-neo-loxP)
Price 
Description The loxP-PGK-gb2-neo-loxP template is designed to allow neomycin/kanamycin selection in prokaryotic and eukaryotic cells.
The loxP-PGK-gb2-neo-loxP template encodes the neomycin/kanamycin resistance gene which combines a prokaryotic promoter (gb2) for expression of kanamycin resistance in E.coli with a eukaryotic promoter (PGK) for expression of neomycin resistance in mammalian cells.
The prokaryotic promoter gb2 is a slightly modified version of the Em7 promoter. It mediates higher transcription efficiency than the normally used Tn5 promoter. The promoter of the mouse Phosphoglucokinase gene (PGK) is used as eukaryotic promoter. A synthetic polyadenylation signal terminates the kanamycin/neomycin expression. The cassette is flanked by loxP sites for later excision by Cre recombinase. Unique NotI and XhoI sites flank the cassette for convenient cloning with restriction sites.
Using the provided PCR template one can easily create a loxP-PGK-gb2-neo-loxP cassette flanked by any restriction sites to clone the cassette into the vector of choice. The restriction sites can be introduced by adding the corresponding sequence in the PCR primer. The template can easily be used to generate targeting constructs mediated by a single Red/ET Recombination step.
The loxP-PGK-gb2-neo-loxP template is not linear but plasmid based (3446bp in size). Due to its R6K origin it can not replicate in most E.coli strains. The PCR product can therefore be used directly for downstream applications without any further purification.
Info Gene Bridges GmbH
Commercial Centre
Im Neuenheimer Feld 584
69120 Heidekberg, Germany
Customer Service: +49 (0)6221 13708 10
Fax Number: +49 (0)6221 13708 29
Web Site: http://www.Genebridges.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. FRT flanked, Pro and Euk. Neomycin Selection Cassette (FRT-PGK-gb2-neo-FRT) from Gene Bridges GmbH
2. FRT flanked, Pro and Euk. Neomycin Selection Cassette plus loxP site(FRT-PGK-gb2-neo-FRT-loxP) from Gene Bridges GmbH
3. EasySep Biotin Selection Kit, Other from StemCell Technologies, Inc.
4. EasySep PE Selection Kit, Other from StemCell Technologies, Inc.
5. EasySep PE Selection Kit, Other, Starter Kit from StemCell Technologies, Inc.
6. EasySep FITC Selection Kit, Other from StemCell Technologies, Inc.
7. loxP flanked Chloramphenicol Selection Cassette (loxP-cm-loxP) from Gene Bridges GmbH
8. FRT flanked Ampicillin Selection Cassette (FRT-amp-FRT) from Gene Bridges GmbH
9. loxP flanked Ampicillin Selection Cassette (loxP-amp-loxP) from Gene Bridges GmbH
10. FailSafe PCR PreMix Selection Kit from EPICENTRE Biotechnologies
11. Trans-Blot Plus Gel and Cassette Assembly Tray from Bio-Rad
... The potential applications for the use of ... gene expression via the RNA interference,(RNAi) pathway ... and molecular biology fields. siRNA for experimental ... or enzymatically, and then transfected into the ...
... unique collision reaction interface (CRI) system*, ... troublesome spectroscopic interferences. The result is ... the worry of complex multipole or ... And, by coupling autotuning of all ...
... Uncover the biological data hidden in your ... flexible manufacturing allows you to create your own ... Design at your own pace and receive delivery ... world. eArray is a secure, online array creation ...
... The high-performance triple quadrupole 1200L ... in today's cost-conscious environment - incorporating ... solutions for all liquid chromatography analytical ... (API) interface and the Transmission Quadrupole ...
Biology Products:
(Date:4/15/2016)... -- A new partnership announced today will help life ... a fraction of the time it takes today, ... insurance policies to consumers without requiring inconvenient and ... rapid testing (A1C, Cotinine and HIV) and higi,s ... pulse, BMI, and activity data) available at local ...
(Date:3/31/2016)... 2016  Genomics firm Nabsys has completed a financial ... Bready , M.D., who returned to the company in ... leadership team, including Chief Technology Officer, John Oliver ... Nurnberg and Vice President of Software and Informatics, ... Dr. Bready served as CEO of Nabsys from ...
(Date:3/21/2016)... March 22, 2016 Unique ... passcodes for superior security   ... provider of secure digital communications services, today announced it ... and offer enterprise customers, particularly those in the Financial ... and voice authentication within a mobile app, alongside, and ...
Breaking Biology News(10 mins):
(Date:4/27/2016)... ... April 27, 2016 , ... Global Stem Cells ... of an Asia-Pacific Symposium as other research and development initiatives for potential stem cell ... and top Global Stem Cells Group executives began meeting to establish a working agenda ...
(Date:4/26/2016)... ... April 27, 2016 , ... ... Roca Rothgerber Christie LLP as an associate in the firm’s Intellectual Property practice ... electrical, mechanical and electromechanical patent applications. He has an electrical engineering and computer ...
(Date:4/26/2016)... ... April 26, 2016 , ... Heidelberg Instruments, a ... technology innovation for its Volume Pattern Generator (VPG) line of lithography systems. The ... photomasks as well as a solution for mid volume direct write lithography applications. ...
(Date:4/26/2016)... ... April 26, 2016 , ... BioFactura, Inc ., ... A round of financing. Healthy investor interest drove significant oversubscription of the original ... biologics, known as biosimilars, to the advanced preclinical stages. , Chief Executive Officer and ...
Breaking Biology Technology: