dotLab System, Dual Pump from Axela Biosensors

Protein-specific capture molecules are immobilized on the dotLab Sensor surface in eight distinct locations, or assay spots. The capture molecules within each spot are not randomly distributed, but are immobilized in a series of parallel lines that produces a specific diffraction pattern when illuminated with a laser. The sensor surface forms the base of a low-volume flow cell. When a flowing stream of sample is introduced into the flow cell, target molecules bind to the assay spots, resulting in an increased diffraction signal. The dotLab System uses the intensity of the diffraction signal to generate real-time binding curves. The illumination and detection beams never pass through the sample, which makes the dotLab System ideal for the detection of proteins in complex biological samples such as serum, plasma, and crude cell lysates.

Axela's dotLab System was designed with the bench researcher in mind. Monitoring biomolecular interactions and developing immunoassays can be complex undertakings, and more often than not it is difficult to find tools that simplify the process.

The dotLab System is a cost-effective platform that has the format flexibility to provide a quick and simple solution to both assay development and routine analysis. Scientists can determine protein levels and characterize protein interactions at their bench. Real-time binding data are generated at each stage of the assay process, eliminating the need to extrapolate from end-point results. Armed with the whole picture, researchers can accelerate iterative development, leading to more accurate investigative decisions. A thorough understanding of complex interactions translates to higher confidence in assay results.

Axela's benchtop dotLab System and disposable dotLab Sensors are an alternative, affordable platform for use in immunoassay and biomolecular interaction studies. To learn more about the system and its applications, or to obtain pricing information, please call or email to have a technical representative in the US or Canada contact you.