Navigation Links
beta-Agarase from USB Corp.

Productsbeta-Agarase from USB Corp.
Company USB Corp.
Item beta-Agarase
Description Source: Pseudomonas atlantica
Beta-Agarase is useful in recovering DNA and RNA fragments from low-melting-temperature agarose. Beta-Agarase digests the agarose into oligo- and disaccharides.
Tested User Friendly
Heat Inactivation: 65C, 15 min
Molecular Weight: 30,000 Da
Optimum pH: 6.5
Tested for contaminating endonucleases, exonucleases and ribonucleases.
Storage Buffer:
50mM Bis-Tris-HCl (pH 6.5), 1mM EDTA, 50% glycerol.
Shipping and Storage:
Shipped on dry ice. Store at -20C.
Assay Conditions:
Reaction mixture contains 10mM Bis-Tris-HCI (pH 6.5) and 1mM EDTA.
Unit Definition:
One unit is the amount of enzyme required to digest 200 l of melted low-melting-point agarose to oligo- and disaccharides in 1 hr at 40C.
1 unit/l
Tested User FriendlyTM Functional Test:
Isolation of linearized plasmid from low-melting agarose, > 50% recovery.


Quantitative recovery of DNA from low-melting point agarose gels.


*Equilibrate DNA/agarose in at least 2 volumes of 1X Beta-Agarase Reaction Buffer for approximately 20 min.
*Spin briefly and remove the supernatant.
*Incubate DNA/agarose at 70C for 10 min to melt the agarose.
*Cool to 40C and maintain the temperature at 37C.
*Add an equal volume of 1X Beta-Agarase Reaction Buffer.
*Add one unit of Beta-Agarase for every 50 l of agarose gel. Do not include the volume of the buffer for this determination.
*Incubate the enzyme/DNA/agarose for 1-18 hours at 37C. The treated DNA may be used directly for ligation, restriction digests, etc. or further purified as in steps 8-12.
*Extract once with buffer equilibrated phenol.
*Extract once with buffer equilibrated phenol:chloroform (1:1).
*Extract twice with chloroform:isoamyl alcohol (24:1).
*Add 2 volumes of TE (pH 7.6) to prevent precipitation of the oligosaccharides. Precipitate with 0.05 volumes of NaCl and 2 volumes of 95% ethanol. Incubate at -20C for 30 min, then spin in a microcentrifuge. Wash the pellet twice with 70% ethanol.
*The DNA solution may be used directly for ligation, restriction digests, transformation and sequencing or the purified DNA may be concentrated.


*YAPHE, W. (1957) Can. J. Microbiol. 3, 987-993.

*BURMEISTER, M. AND LEHRACH, H. (1986) Nature 324, 582-585.

Functionally Tested 10X Beta-Agarase Reaction Buffer (1 ml included, PN 73432):
100mM Bis-Tris-HCl (pH 6.5), 10mM EDTA
Info USB Corp.USB Corp.
26111 Miles Road
Cleveland Ohio 44128

Call USB Corp. to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-216-245-1829
Customer Service: 216.765.5000
Fax Number: 216.464.5075
Web Site:
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. S-Acetyl Coenzyme A Lithium Salt from USB Corp.
2. Mut S from USB Corp.
3. Thermo Sequenase Dye Primer Manual Cycle Sequencing Kit from USB Corp.
4. Phenol Red, Free Acid from USB Corp.
5. Dithiothreitol from USB Corp.
6. Bromophenol Blue from USB Corp.
7. Xylene Cyanol FF from USB Corp.
8. Ligase, T4 DNA from USB Corp.
9. Human Topoisomerase II, Alpha from USB Corp.
10. Calf Topoisomerase I from USB Corp.
11. M-MLV Reverse Transcriptase from USB Corp.
These macrocarriers are for the biolistic PDS-1000 He and Hepta systems. Supplied as 500 macrocarriers....
Anti Human S14G-Humanin Polyclonal Antibody...
... Science Major Science MS 300V (Cat. No. ... designed to meet most electrophoresis needs in ... Major Science MS 300V power supply ... instruments used in electrophoresis. 4 pairs of ...
Biology Products:
(Date:11/2/2015)... 2015  SRI International has been awarded a contract ... services to the National Cancer Institute (NCI) PREVENT Cancer ... expertise, modern testing and support facilities, and analytical instrumentation ... toxicology studies to evaluate potential cancer prevention drugs. ... Cancer Drug Development Program is an NCI-supported pipeline to ...
(Date:10/29/2015)... JOLLA, Calif. , Oct. 29, 2015  The ... a new report titled, "DNA Synthesis and Biosecurity: Lessons ... well the Department of Health and Human Services guidance ... issued in 2010. --> ... but it also has the potential to pose unique ...
(Date:10/29/2015)... ARBOR, Mich. , Oct. 29, 2015 /PRNewswire/ ... Eurofins Genomics for U.S. distribution of its DNA ... DNA-seq kit and Rubicon,s new ThruPLEX Plasma-seq kit. ... to enable the preparation of NGS libraries for ... plasma for diagnostic and prognostic applications in cancer ...
Breaking Biology News(10 mins):
(Date:11/27/2015)... November 27, 2015 ... popularity of companion diagnostics is one of ... market with pharmaceutical companies and diagnostic manufacturers ... tests. . --> ... report on global cancer biomarkers market spread ...
(Date:11/25/2015)... , Nov. 25, 2015  PharmAthene, Inc. (NYSE MKT: ... adopted a stockholder rights plan (Rights Plan) in an ... loss carryforwards (NOLs) under Section 382 of the Internal ... --> PharmAthene,s use of its NOLs could be ... as defined in Section 382 of the Code. In ...
(Date:11/25/2015)... , Nov. 25, 2015  Neurocrine Biosciences, Inc. ... Gorman , President and CEO of Neurocrine Biosciences, will ... Conference in New York . ... visit the website approximately 5 minutes prior to the ... replay of the presentation will be available on the ...
(Date:11/25/2015)... PORTLAND, Oregon , November 25, 2015 /PRNewswire/ ... Deep Market Research Report is a professional and ... Genomics industry.      (Logo: ... basic overview of the industry including definitions, classifications, ... analysis is provided for the international markets including ...
Breaking Biology Technology: