Navigation Links
beta-Agarase from USB Corp.

Productsbeta-Agarase from USB Corp.
Company USB Corp.
Item beta-Agarase
Price 
Description Source: Pseudomonas atlantica
Description:
Beta-Agarase is useful in recovering DNA and RNA fragments from low-melting-temperature agarose. Beta-Agarase digests the agarose into oligo- and disaccharides.
Tested User Friendly
Properties:
Heat Inactivation: 65C, 15 min
Molecular Weight: 30,000 Da
Optimum pH: 6.5
Purity:
Tested for contaminating endonucleases, exonucleases and ribonucleases.
Storage Buffer:
50mM Bis-Tris-HCl (pH 6.5), 1mM EDTA, 50% glycerol.
Shipping and Storage:
Shipped on dry ice. Store at -20C.
Assay Conditions:
Reaction mixture contains 10mM Bis-Tris-HCI (pH 6.5) and 1mM EDTA.
Unit Definition:
One unit is the amount of enzyme required to digest 200 l of melted low-melting-point agarose to oligo- and disaccharides in 1 hr at 40C.
Concentration:
1 unit/l
Tested User FriendlyTM Functional Test:
Isolation of linearized plasmid from low-melting agarose, > 50% recovery.

Applications:

*
Quantitative recovery of DNA from low-melting point agarose gels.

PROTOCOL FOR RECOVERING FRAGMENTS FROM LOW-MELT AGAROSE

*Equilibrate DNA/agarose in at least 2 volumes of 1X Beta-Agarase Reaction Buffer for approximately 20 min.
*Spin briefly and remove the supernatant.
*Incubate DNA/agarose at 70C for 10 min to melt the agarose.
*Cool to 40C and maintain the temperature at 37C.
*Add an equal volume of 1X Beta-Agarase Reaction Buffer.
*Add one unit of Beta-Agarase for every 50 l of agarose gel. Do not include the volume of the buffer for this determination.
*Incubate the enzyme/DNA/agarose for 1-18 hours at 37C. The treated DNA may be used directly for ligation, restriction digests, etc. or further purified as in steps 8-12.
*Extract once with buffer equilibrated phenol.
*Extract once with buffer equilibrated phenol:chloroform (1:1).
*Extract twice with chloroform:isoamyl alcohol (24:1).
*Add 2 volumes of TE (pH 7.6) to prevent precipitation of the oligosaccharides. Precipitate with 0.05 volumes of NaCl and 2 volumes of 95% ethanol. Incubate at -20C for 30 min, then spin in a microcentrifuge. Wash the pellet twice with 70% ethanol.
*The DNA solution may be used directly for ligation, restriction digests, transformation and sequencing or the purified DNA may be concentrated.

References:

*YAPHE, W. (1957) Can. J. Microbiol. 3, 987-993.

*BURMEISTER, M. AND LEHRACH, H. (1986) Nature 324, 582-585.

Functionally Tested 10X Beta-Agarase Reaction Buffer (1 ml included, PN 73432):
100mM Bis-Tris-HCl (pH 6.5), 10mM EDTA
Info USB Corp.USB Corp.
26111 Miles Road
Cleveland Ohio 44128


Call USB Corp. to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-216-245-1829
Customer Service: 216.765.5000
Fax Number: 216.464.5075
Web Site: http://www.usbweb.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. S-Acetyl Coenzyme A Lithium Salt from USB Corp.
2. Mut S from USB Corp.
3. Thermo Sequenase Dye Primer Manual Cycle Sequencing Kit from USB Corp.
4. Phenol Red, Free Acid from USB Corp.
5. Dithiothreitol from USB Corp.
6. Bromophenol Blue from USB Corp.
7. Xylene Cyanol FF from USB Corp.
8. Ligase, T4 DNA from USB Corp.
9. Human Topoisomerase II, Alpha from USB Corp.
10. Calf Topoisomerase I from USB Corp.
11. M-MLV Reverse Transcriptase from USB Corp.
... for Shimadzu Phoretix 2D, Expression and Evolution ... for comparative gel analysis studies in determining ... add on component Oracle data ... release 2 client software). Supports ...
... units/µL ,• High specific activity and processivity ... 'A' overhang ,• Processes ,A ... activity in primer extension and other molecular ... the enzyme, each lot has been functionally ...
Our original highly-purified, robust Taq Polymerase which delivers consistently high yields and minimal background...
... DNA Polymerase is the most thoroughly characterized ... testimony to its overall utility and efficacy. ... ideal for PCR applications. , It ... ,, AmpliTaq DNA Polymerase is a ...
Biology Products:
(Date:3/29/2017)... 2017  higi, the health IT company that operates ... America , today announced a Series B investment ... EveryMove. The new investment and acquisition accelerates higi,s strategy ... transform population health activities through the collection and workflow ... higi collects and secures data today on behalf of ...
(Date:3/27/2017)... N.Y. , March 27, 2017  Catholic ... Information and Management Systems Society (HIMSS) Analytics for ... EMR Adoption Model sm . In addition, CHS ... of U.S. hospitals using an electronic medical record ... for its high level of EMR usage in ...
(Date:3/24/2017)... Mar 24, 2017 Research and Markets has ... Market Analysis & Trends - Industry Forecast to 2025" report ... ... at a CAGR of around 15.1% over the next decade to ... report analyzes the market estimates and forecasts for all the given ...
Breaking Biology News(10 mins):
(Date:10/10/2017)... ... October 10, 2017 , ... ... company advancing targeted antibody-drug conjugate (ADC) therapeutics, today confirmed licensing rights that ... Polymerized Liposomal Nanoparticle), a technology developed in collaboration with Children’s Hospital Los ...
(Date:10/10/2017)... ... October 10, 2017 , ... Dr. Bob Harman, founder and CEO of ... Club. The event entitled “Stem Cells and Their Regenerative Powers,” was ... Dr. Harman, DVM, MPVM was joined by two human doctors: Peter B. Hanson, M.D., ...
(Date:10/10/2017)... CRUZ, Calif. , Oct. 10, 2017 ... grant from the NIH to develop RealSeq®-SC (Single Cell), ... kit for profiling small RNAs (including microRNAs) from single ... Analysis Program highlights the need to accelerate development of ... "New techniques for measuring ...
(Date:10/10/2017)... ... October 10, 2017 , ... The Pittcon Program ... honoring scientists who have made outstanding contributions to analytical chemistry and ... 2018, the world’s leading conference and exposition for laboratory science, which will be ...
Breaking Biology Technology: