Navigation Links
beta-Agarase from USB Corp.

Productsbeta-Agarase from USB Corp.
Company USB Corp.
Item beta-Agarase
Description Source: Pseudomonas atlantica
Beta-Agarase is useful in recovering DNA and RNA fragments from low-melting-temperature agarose. Beta-Agarase digests the agarose into oligo- and disaccharides.
Tested User Friendly
Heat Inactivation: 65C, 15 min
Molecular Weight: 30,000 Da
Optimum pH: 6.5
Tested for contaminating endonucleases, exonucleases and ribonucleases.
Storage Buffer:
50mM Bis-Tris-HCl (pH 6.5), 1mM EDTA, 50% glycerol.
Shipping and Storage:
Shipped on dry ice. Store at -20C.
Assay Conditions:
Reaction mixture contains 10mM Bis-Tris-HCI (pH 6.5) and 1mM EDTA.
Unit Definition:
One unit is the amount of enzyme required to digest 200 l of melted low-melting-point agarose to oligo- and disaccharides in 1 hr at 40C.
1 unit/l
Tested User FriendlyTM Functional Test:
Isolation of linearized plasmid from low-melting agarose, > 50% recovery.


Quantitative recovery of DNA from low-melting point agarose gels.


*Equilibrate DNA/agarose in at least 2 volumes of 1X Beta-Agarase Reaction Buffer for approximately 20 min.
*Spin briefly and remove the supernatant.
*Incubate DNA/agarose at 70C for 10 min to melt the agarose.
*Cool to 40C and maintain the temperature at 37C.
*Add an equal volume of 1X Beta-Agarase Reaction Buffer.
*Add one unit of Beta-Agarase for every 50 l of agarose gel. Do not include the volume of the buffer for this determination.
*Incubate the enzyme/DNA/agarose for 1-18 hours at 37C. The treated DNA may be used directly for ligation, restriction digests, etc. or further purified as in steps 8-12.
*Extract once with buffer equilibrated phenol.
*Extract once with buffer equilibrated phenol:chloroform (1:1).
*Extract twice with chloroform:isoamyl alcohol (24:1).
*Add 2 volumes of TE (pH 7.6) to prevent precipitation of the oligosaccharides. Precipitate with 0.05 volumes of NaCl and 2 volumes of 95% ethanol. Incubate at -20C for 30 min, then spin in a microcentrifuge. Wash the pellet twice with 70% ethanol.
*The DNA solution may be used directly for ligation, restriction digests, transformation and sequencing or the purified DNA may be concentrated.


*YAPHE, W. (1957) Can. J. Microbiol. 3, 987-993.

*BURMEISTER, M. AND LEHRACH, H. (1986) Nature 324, 582-585.

Functionally Tested 10X Beta-Agarase Reaction Buffer (1 ml included, PN 73432):
100mM Bis-Tris-HCl (pH 6.5), 10mM EDTA
Info USB Corp.USB Corp.
26111 Miles Road
Cleveland Ohio 44128

Call USB Corp. to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-216-245-1829
Customer Service: 216.765.5000
Fax Number: 216.464.5075
Web Site:
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. S-Acetyl Coenzyme A Lithium Salt from USB Corp.
2. Mut S from USB Corp.
3. Thermo Sequenase Dye Primer Manual Cycle Sequencing Kit from USB Corp.
4. Phenol Red, Free Acid from USB Corp.
5. Dithiothreitol from USB Corp.
6. Bromophenol Blue from USB Corp.
7. Xylene Cyanol FF from USB Corp.
8. Ligase, T4 DNA from USB Corp.
9. Human Topoisomerase II, Alpha from USB Corp.
10. Calf Topoisomerase I from USB Corp.
11. M-MLV Reverse Transcriptase from USB Corp.
... Conjugation Kits are based on well-established ... are consistent and reproducible. This kit ... your protein or antibody, including ready-to-use ... Activated HRP is supplied ready-to-use ...
... Acridinium NHS ester can be ... nucleic acids. The covalently bound ... chemiluminescence in the presence of ... proteins can be used as ...
... Automated instrument designed to hold up to ... The robust and easy to use design ensures ... with regular cyclic stretching or compression. This unit ... of the strain to produce 64 unique strain ...
... The Genome-pULSe Kit delivers a novel ... direct (non-enzymatic) labeling of whole genome DNA ... designed to provide a quick and highly ... analysis. Genome-pULSe consists of a REPLI-g Mini ...
Biology Products:
(Date:3/23/2016)... 2016 Einzigartige ... und Stimmerkennung mit Passwörtern     ... MESG ), ein führender Anbieter digitaler Kommunikationsdienste, ... SpeechPro zusammenarbeitet, um erstmals dessen Biometrietechnologie einzusetzen. ... Möglichkeit angeboten, im Rahmen mobiler Apps neben ...
(Date:3/22/2016)... and SANDY, Utah , March ... operates the highest sample volume laboratory in ... and UNIConnect, leaders in clinical sequencing informatics and molecular ... of a project to establish the informatics infrastructure for ... NSO has been contracted by the Ontario Ministry ...
(Date:3/18/2016)... LONDON , March 18, 2016 ... Established Suppliers of Biometrics, ICT, Manned & Unmanned Vehicles, Physical ... & security companies in the border security market and ... and Europe has led ... your companies improved success. --> defence & ...
Breaking Biology News(10 mins):
(Date:6/27/2016)... ... June 27, 2016 , ... ... Amgen, will join the faculty of the University of North Carolina Kenan-Flagler ... of strategy and entrepreneurship at UNC Kenan-Flagler, with a focus on the school’s ...
(Date:6/24/2016)... discussions on a range of subjects including policies, debt and ... Poloz. Speaking at a lecture to the Canadian ... to the country,s inflation target, which is set by both ... "In certain areas there needs to be ... why not sit down and address strategy together?" ...
(Date:6/24/2016)... ... ... Researchers at the Universita Politecnica delle Marche in Ancona combed medical journal articles ... findings are the subject of a new article on the Surviving Mesothelioma website. ... blood, lung fluid or tissue of mesothelioma patients that can help point doctors to ...
(Date:6/23/2016)... , June 23, 2016   Boston ... of novel compounds designed to target cancer stemness ... has been granted Orphan Drug Designation from the ... treatment of gastric cancer, including gastroesophageal junction (GEJ) ... inhibitor designed to inhibit cancer stemness pathways by ...
Breaking Biology Technology: