Navigation Links
beta-Agarase from USB Corp.

Productsbeta-Agarase from USB Corp.
Company USB Corp.
Item beta-Agarase
Price 
Description Source: Pseudomonas atlantica
Description:
Beta-Agarase is useful in recovering DNA and RNA fragments from low-melting-temperature agarose. Beta-Agarase digests the agarose into oligo- and disaccharides.
Tested User Friendly
Properties:
Heat Inactivation: 65C, 15 min
Molecular Weight: 30,000 Da
Optimum pH: 6.5
Purity:
Tested for contaminating endonucleases, exonucleases and ribonucleases.
Storage Buffer:
50mM Bis-Tris-HCl (pH 6.5), 1mM EDTA, 50% glycerol.
Shipping and Storage:
Shipped on dry ice. Store at -20C.
Assay Conditions:
Reaction mixture contains 10mM Bis-Tris-HCI (pH 6.5) and 1mM EDTA.
Unit Definition:
One unit is the amount of enzyme required to digest 200 l of melted low-melting-point agarose to oligo- and disaccharides in 1 hr at 40C.
Concentration:
1 unit/l
Tested User FriendlyTM Functional Test:
Isolation of linearized plasmid from low-melting agarose, > 50% recovery.

Applications:

*
Quantitative recovery of DNA from low-melting point agarose gels.

PROTOCOL FOR RECOVERING FRAGMENTS FROM LOW-MELT AGAROSE

*Equilibrate DNA/agarose in at least 2 volumes of 1X Beta-Agarase Reaction Buffer for approximately 20 min.
*Spin briefly and remove the supernatant.
*Incubate DNA/agarose at 70C for 10 min to melt the agarose.
*Cool to 40C and maintain the temperature at 37C.
*Add an equal volume of 1X Beta-Agarase Reaction Buffer.
*Add one unit of Beta-Agarase for every 50 l of agarose gel. Do not include the volume of the buffer for this determination.
*Incubate the enzyme/DNA/agarose for 1-18 hours at 37C. The treated DNA may be used directly for ligation, restriction digests, etc. or further purified as in steps 8-12.
*Extract once with buffer equilibrated phenol.
*Extract once with buffer equilibrated phenol:chloroform (1:1).
*Extract twice with chloroform:isoamyl alcohol (24:1).
*Add 2 volumes of TE (pH 7.6) to prevent precipitation of the oligosaccharides. Precipitate with 0.05 volumes of NaCl and 2 volumes of 95% ethanol. Incubate at -20C for 30 min, then spin in a microcentrifuge. Wash the pellet twice with 70% ethanol.
*The DNA solution may be used directly for ligation, restriction digests, transformation and sequencing or the purified DNA may be concentrated.

References:

*YAPHE, W. (1957) Can. J. Microbiol. 3, 987-993.

*BURMEISTER, M. AND LEHRACH, H. (1986) Nature 324, 582-585.

Functionally Tested 10X Beta-Agarase Reaction Buffer (1 ml included, PN 73432):
100mM Bis-Tris-HCl (pH 6.5), 10mM EDTA
Info USB Corp.USB Corp.
26111 Miles Road
Cleveland Ohio 44128


Call USB Corp. to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-216-245-1829
Customer Service: 216.765.5000
Fax Number: 216.464.5075
Web Site: http://www.usbweb.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. S-Acetyl Coenzyme A Lithium Salt from USB Corp.
2. Mut S from USB Corp.
3. Thermo Sequenase Dye Primer Manual Cycle Sequencing Kit from USB Corp.
4. Phenol Red, Free Acid from USB Corp.
5. Dithiothreitol from USB Corp.
6. Bromophenol Blue from USB Corp.
7. Xylene Cyanol FF from USB Corp.
8. Ligase, T4 DNA from USB Corp.
9. Human Topoisomerase II, Alpha from USB Corp.
10. Calf Topoisomerase I from USB Corp.
11. M-MLV Reverse Transcriptase from USB Corp.
... Quality 96 Well BAC Prep; ... preparation from 1.5ml 2xYT cultures; ... automated fluorescent sequencing for small ... ProPrep strategy which minimizes shearing ...
... monoclonal [SPM278] to 67kD Laminin Receptor, prediluted ... N592 live cells (Human). Reactivity / ... tested in other species. Background Information ... of basement membrane, displays multiple biological activities ...
Mouse Anti-Lamin B...
TLN2 Antibody...
Biology Products:
(Date:8/7/2018)... ... August 07, 2018 , ... Nanomedical ... announces a new publication titled, “Synthetic Molecules for Disruption of the Myc ... prominently features use of Agile R100, described as a “ field-effect transistor (Bio-FET) ...
(Date:8/1/2018)... ... , ... Dr. Asher Kimchi, Founder and Chairman of the International Academy ... at the 23rd World Congress on Heart Disease held in Boston, MA, USA. In ... Distinguished Fellowship Awards. , Dr. Asher Kimchi, together with Chairman Dr. John A. Elefteriades, ...
(Date:7/31/2018)... ... July 31, 2018 , ... ACEA Biosciences, is a privately owned biotechnology company ... introduced the xCELLigence RTCA S16, which the ideal entry level model for exploratory studies ... S16can be placed in any standard CO2 tissue culture incubator. The user friendly ...
Breaking Biology News(10 mins):
(Date:8/14/2018)... ... August 14, 2018 , ... ... at the 11th Congress of the Chinese Association of Orthopaedic Surgeons (CAOS) held ... Fule Science & Technology Development , Beijing, and Invibio Biomaterial Solutions ...
(Date:8/9/2018)... ... August 08, 2018 , ... ActX introduces GenoACT(sm), a Genetic ... by a talented scientific team that includes geneticists, PharmD’s, genetic counselors, and physicians ... on the medical impact of a patient’s genetic variants , 2. Educational information ...
(Date:8/9/2018)... ... August 09, 2018 , ... ... management (RCM), patient engagement and analytics software and services for physician practices, announced ... in its twenty-year history. After back-to-back record quarters in Q2 and Q3 last ...
(Date:8/7/2018)... (PRWEB) , ... August 06, 2018 , ... ... sciences and diagnostics companies, announces the placement of Dr. Paul Harney as President, ... Prescient’s North America growth strategy and enhancing client satisfaction. He was also named ...
Breaking Biology Technology: