|Products||Zero Blunt TOPO Kit for Sequencing with One Shot Mach1 T1 Phage-Resistant Chemically Competent E. coli from Invitrogen|
|Item||Zero Blunt TOPO Kit for Sequencing with One Shot Mach1 T1 Phage-Resistant Chemically Competent E. coli|
|Description||The TOPO Cloning Kits for Sequencing allow 5-minute cloning and subsequent sequencing of PCR products. The kits contain TOPO Cloning vectors with primer sites located as close as 33 base pairs from the PCR product insertion site. This minimizes the amount of vector DNA that needs to be read before reaching the sequence of your insert.vectors are available. The pCR4-TOPO vector provides 3-T overhangs for direct ligation of Taq-amplified PCR products. The pCR4Blunt-TOPO vector provides blunt ends for cloning PCR products amplified with proofreading thermostable polymerases. Each vector is supplied linearized and topoisomerase I-activated enabling 5-minute bench-top ligation yielding recombinants (1). Both vectors feature:|
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