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XpressPack™ Luminescent Amplification Detection System, required for luminescent XpressPack™ from CHEMICON

ProductsXpressPack™ Luminescent Amplification Detection System, required for luminescent XpressPack™ from CHEMICON
Company CHEMICON
Item XpressPack™ Luminescent Amplification Detection System, required for luminescent XpressPack™
Price $263.00
Description The XpressPack Luminescent Detection System is intended for use in the quantitative detection of amplified DNA generated by an in-house validated method for in vitro nucleic acid amplification of a selected target of interest. • The XpressPack Luminescent Detection System is a hybridization capture assay designed to detect amplified nucleic acid specific to the target of interest. Amplification reactions use a biotinylated primer set (included in the XpressPackTM gene-specific kit) to generate the required amplified product (amplicon). A portion of the resultant amplicon is added directly to the capture plate (included in the XpressPack gene-specific kit) and denatured under alkali conditions to separate the two DNA strands. The single-stranded amplicon is captured to the plate via hybridization with a specific oligonucleotide probe immobilized to the reaction well (target specific probe-coated plate included in the XpressPack gene-specific kit). The hybrid complex is then reacted with streptavidin conjugated to AquaLite, a recombinant form of the photoprotein aequorin. Unbound conjugate is removed by washing. When calcium cations are added, a blue light is emitted. This light is quantified using a microplate luminometer; the intensity of the light is directly proportional to the amount of amplicon present in the sample.

  • Components: Denaturation Solution
    Hybridization Buffer
    AquaLite® Wash Buffer, 10X
    AquaLite® Streptavidin Assay Buffer, 10X
    AquaLite® Reconstitution Buffer
    AquaLite® Trigger Buffer, 10X
    AquaLite®:Streptavidin Conjugate
  • Applications: DNA Amplification
Info CHEMICONCHEMICON
CHEMICON International, Inc.
28820 Single Oak Drive
Temecula, CA 92590
Customer Service: 800-437-7500
Tech Support: 800-437-7500
Fax Number: 800-437-7502
Web Site: http://www.chemicon.com
NOTE: Price information is approximate list price and actual prices may vary.

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