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XpressPack™ Colorimetric Amplification Detection System, required for colorimetric XpressPack™ from CHEMICON

ProductsXpressPack™ Colorimetric Amplification Detection System, required for colorimetric XpressPack™ from CHEMICON
Company CHEMICON
Item XpressPack™ Colorimetric Amplification Detection System, required for colorimetric XpressPack™
Price $265.00
Description The CHEMICON XpressPack™ Colorimetric Detection System is intended for use in the qualitative detection of amplified DNA generated by an in-house validated in vitro nucleic acid amplification of a selected target of interest. • The CHEMICON XpressPack™ Colorimetric Detection System is a hybridization capture assay designed to detect amplified nucleic acid specific to the target of interest. Amplification reactions incorporating a biotinylated primer set (included in the XpressPack™ gene-specific kit) generate the required amplification product (amplicon). A portion of the resultant amplicon is added directly to the capture plate and denatured under alkali conditions separating the two DNA strands. The single-stranded amplicon is captured to the plate via hybridization with a specific oligonucleotide probe immobilized to the reaction well (target specific probe-coated plate included in the XpressPack™ gene-specific kit). The hybrid complex is then reacted with streptavidin conjugated to horseradish peroxidase. Unbound conjugate is removed by washing. When TMB substrate is added, a blue color develops. The reaction is stopped by the addition of an acid, changing the color to yellow. This color is quantitated using a microplate reader; the intensity of the color is directly proportional to the amount of amplicon present in the sample.

  • Components: Denaturation Solution
    Hybridization Buffer
    Wash Buffer, 10X
    Strep:HRP Conjugate - Ready to Use
    Substrate (TMB/E)
    Stop Solution
  • Applications: DNA Amplification
Info CHEMICONCHEMICON
CHEMICON International, Inc.
28820 Single Oak Drive
Temecula, CA 92590
Customer Service: 800-437-7500
Tech Support: 800-437-7500
Fax Number: 800-437-7502
Web Site: http://www.chemicon.com
NOTE: Price information is approximate list price and actual prices may vary.

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