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UltraLink Immobilized NeutrAvidin from Pierce Biotechnology, Inc.

ProductsUltraLink Immobilized NeutrAvidin from Pierce Biotechnology, Inc.
Company Pierce Biotechnology, Inc.
Item UltraLink Immobilized NeutrAvidin
Price 
Description When nonspecific binding is a problem in your application, Pierce offers a variety of immobilized NeutrAvidin products as superior alternatives to avidin or streptavidin. NeutrAvidin is a modified avidin derivative that combines several key features to provide a biotin-binding protein with exceptionally low nonspecific binding properties. NeutrAvidin does not contain carbohydrate and has an ideal isoelectric point (6.3) for the best utility in a variety of applications.

For assay development, Pierce has coated NeutrAvidin onto microplates and pre-blocked the plastic surface. Any biotinylated compound can now be attached to the plate and analyzed further. Proteins will retain their structural integrity when bound to a protein instead of bound directly to a plastic surface. The plastic surface may distort the structure of the proteins and prevent accurate detection of the ligand in the assay system. Binding biotinylated compounds to NeutrAvidin provides a stable anchor to the plastic surface.
Applications

  • Immunoprecipitation
  • Binding Biotinylated DNA to coated plates
  • Purifying proteins that bind to biotinylated ligands
Features
  • Carbohydrate Free - Just like streptavidin, NeutrAvidin has no carbohydrate, eliminating nonspecific binding problems due to sugars.
  • No interaction with cell surface molecules - absence of the Arg-Tyr-Asp sequence (present in streptavidin), which mimics the universal cell surface recognition sequence present in a variety of molecules, eliminates cross-reactivity of cell surface molecules.
  • Easily conjugated - Most modified avidins have lysines that are unavailable for coupling. NeutrAvidin's unique preparation preserves lysines, making conjugations easier.
  • Neutral pI - With a pI of 6.3, NeutrAvidin's pI is closer to neutrality than avidin or streptavidin. This eliminates electrostatic interaction which contributes to nonspecific binding.
Less nonspecific binding produces better results and better yields.
Info Pierce Biotechnology, Inc.Pierce Biotechnology, Inc.
Pierce Biotechnology, Inc.
3747 N. Meridian Rd.
P.O. Box 117
Rockford, IL 61105
Customer Service: 815-968-0747
Fax Number: 815-968-8148
Web Site: http://www.piercenet.com/
NOTE: Price information is approximate list price and actual prices may vary.

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