UltraLink Immobilized NeutrAvidin from Pierce Biotechnology, Inc.

For assay development, Pierce has coated NeutrAvidin onto microplates and pre-blocked the plastic surface. Any biotinylated compound can now be attached to the plate and analyzed further. Proteins will retain their structural integrity when bound to a protein instead of bound directly to a plastic surface. The plastic surface may distort the structure of the proteins and prevent accurate detection of the ligand in the assay system. Binding biotinylated compounds to NeutrAvidin provides a stable anchor to the plastic surface.
Applications

• Immunoprecipitation
• Binding Biotinylated DNA to coated plates
• Purifying proteins that bind to biotinylated ligands

• Carbohydrate Free - Just like streptavidin, NeutrAvidin has no carbohydrate, eliminating nonspecific binding problems due to sugars.
• No interaction with cell surface molecules - absence of the Arg-Tyr-Asp sequence (present in streptavidin), which mimics the universal cell surface recognition sequence present in a variety of molecules, eliminates cross-reactivity of cell surface molecules.
• Easily conjugated - Most modified avidins have lysines that are unavailable for coupling. NeutrAvidin's unique preparation preserves lysines, making conjugations easier.
• Neutral pI - With a pI of 6.3, NeutrAvidin's pI is closer to neutrality than avidin or streptavidin. This eliminates electrostatic interaction which contributes to nonspecific binding.