Navigation Links
UBC Fractions Extract from GeneTex

ProductsUBC Fractions Extract from GeneTex
Company GeneTex
Item UBC Fractions Extract
Price $440.00
Description Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Others are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum. The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis, Angelmans syndrome, and Liddle syndrome. The enzyme fractions supplied represent the full compliment of purified conjugation enzymes (E1, E2s, and E3s) that are found in mammalian Fraction II. The enzymes have been tested and shown to work with typical [125I]-labeled substrate proteins such as lysozyme and beta-lactoglobulin. These conjugation fractions contain ubiquitin c-terminal hydrolases. The addition of ubiquitin aldehyde is recommended for the inhibition of UCHs and to improve overall conjugate yield. The supplied fractions do not contain 20S or 26S protein degradation activity. If the substrate being conjugated requires E2 or E3 enzymes not found in Fraction II, the reaction can be supplemented with enzymes from Fraction I. Includes complimentary fractions for 2-5 conjugation reactions depending on conditions.

  • Type: Proteins and peptides
  • Form: 400 g in 50 mM HEPES, pH 7.6, containing 1 mM DTT.
Info GeneTexGeneTex
14785 Omicron Dr. Suite101
San Antonio, TX 78245
Customer Service: (877) 436-3839
Fax Number: (210) 677-8843
Web Site: http://www.genetex.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Human CALCA EIA (Enzyme immunoassay) Kit, Host: Rabbit ; Extraction-free from BACHEM
2. 26S Proteasome Fraction Extract from GeneTex
3. Extracti-Gel D Detergent Removing Gel AffinityPak Columns from Pierce Biotechnology, Inc.
4. Ubiquitin/Proteasome Fraction II Extract from GeneTex
5. 26S Proteasome Fraction Extract from GeneTex
6. IRS Extract from GeneTex
7. Ubiquitin Fraction I Extract from GeneTex
8. Feature Extraction Software; 2-year License for Not-For-Profit/Academic from Agilent Technologies
9. Feature Extraction Software; 1-year license for Commercial from Agilent Technologies
10. Feature Extraction Software; 1-year License for Not-For-Profit/Academic from Agilent Technologies
11. Panorama Mouse/Rat Tissue Extract Protein Array Kit from Sigma-Aldrich
Shake 'n' Stack Oven - New Model (includes 10 bottle capacity rotisserie, adjustable feet, drip tray, manual PLUS Shaker Platform)...
... Polymerase I from E. coli ... The enzyme also contains both ... 5'?3' exonuclease activity enables the ... gaps in the DNA as ...
DNase I (Deoxyribonuclease I) digests single- and double-stranded DNA to oligodeoxyribonucleotides. Ribonuclease activity has been reduced to non-detectable levels....
... Calf thymus Description: Topoisomerase I relaxes ... transient breakage and rejoining of phosphodiester bonds (1,2) ... molecules to be differentiated by a single nucleotide ... is active in the absence of Mn 2+ ...
Biology Products:
(Date:5/18/2013)... Research Foundation is honored to announce the first AGA-Caroline ... which will support Andrew D. Rhim, MD, from the ... Philadelphia, as he furthers his research on the role ... , "The AGA Research Foundation is humbled to issue ... AGA Legacy Society members," said Nicholas F. LaRusso, MD, ...
(Date:5/18/2013)... presented today at Digestive Disease Week (DDW) explores new ... impact of coffee on autoimmune disease and palliative care ... has been associated with reduced risk of fibrosis, a ... of java each month also correlate with lower risk ... Mayo Clinic, Rochester, MN, linked coffee consumption with reduced ...
(Date:5/18/2013)... (May 18, 2013) Research presented at Digestive ... digestive health through diet and lifestyle. , ... by reduced muscle strength, fatigue and poor quality ... patients are in remission. A randomized, double blind, ... vitamin D supplementation corresponded to significant relief of ...
Breaking Biology News(10 mins):AGA Research Foundation grant furthers digestive cancer research 2Coffee consumption associated with reduced risk of autoimmune liver disease 2Research examines new methods for managing digestive health 2Research examines new methods for managing digestive health 3
... the world today agreed at a meeting in Canada to ... in leadership related to global water issues. Saying ... say the panel will work to elevate the issue,s political ... The 20 members of the InterAction Council attending this ...
... structures on the smallest possible scales using molecules ... physically and conceptually challenging. An interdisciplinary team of researchers ... method of computationally selecting the best of these blocks, ... making biological structures. The team was led by ...
... University shows that so-called biodegradable products are likely doing ... releasing a powerful greenhouse gas as they break down. ... are broken down in landfills by microorganisms that then ... paper describing the research and professor and head of ...
Cached Biology News:Void in leadership on world water crisis cited by 20 former heads of government 2Penn researchers help nanoscale engineers choose self-assembling proteins 2Penn researchers help nanoscale engineers choose self-assembling proteins 3Study: Biodegradable products may be bad for the environment 2
(Date:5/19/2013)... Boston, MA (PRWEB) May 19, 2013 ... providing social media training online, has proudly announced that ... for small business has surpassed 175,000 views on YouTube. ... free social media and SEO tips and training for ... see my students find the YouTube tips helpful for ...
(Date:5/17/2013)... May 17, 2013 Topricin Pain ... Inc., has been listed among nationally recommended over-the-counter ... initiative conducted by "U.S. News" Health in collaboration ... the “No. 1 Pharmacists Recommended designation” in ... national OTC brands, and is the only homeopathic ...
(Date:5/17/2013)... Dr. Sparano is Professor of Medicine and Professor ... Einstein College of Medicine and Associate Chairman of the ... He is also Associate Director for Clinical Research at ... Cancer Working Group, a multidisciplinary group of physicians and ... serves as Vice Chair of the National Cancer Institute ...
(Date:5/17/2013)... (PRWEB) May 17, 2013 Oven Industries ... capabilities. The 5R6-900 temperature controller has many user-friendly benefits. ... plugged into the wall as a self-contained temperature ... This distinctive detail makes the device unique, as well ... controller can also be used universally, which allows the ...
Breaking Biology Technology:YouTube Channel on SEO Tips Surpasses 175,000 Views, Announces JM Internet Group 2Pharmacists' Picks: Topical BioMedics' Topricin Listed on Top Recommended OTC Health Products 2Joseph A. Sparano, MD, Named Vice Chair of ECOG-ACRIN Cancer Research Group 2Joseph A. Sparano, MD, Named Vice Chair of ECOG-ACRIN Cancer Research Group 3New Temperature Control Products Announced by Oven Industries Inc. 2