Navigation Links
UBC Fractions Extract from GeneTex

ProductsUBC Fractions Extract from GeneTex
Company GeneTex
Item UBC Fractions Extract
Price $440.00
Description Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Others are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum. The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis, Angelmans syndrome, and Liddle syndrome. The enzyme fractions supplied represent the full compliment of purified conjugation enzymes (E1, E2s, and E3s) that are found in mammalian Fraction II. The enzymes have been tested and shown to work with typical [125I]-labeled substrate proteins such as lysozyme and beta-lactoglobulin. These conjugation fractions contain ubiquitin c-terminal hydrolases. The addition of ubiquitin aldehyde is recommended for the inhibition of UCHs and to improve overall conjugate yield. The supplied fractions do not contain 20S or 26S protein degradation activity. If the substrate being conjugated requires E2 or E3 enzymes not found in Fraction II, the reaction can be supplemented with enzymes from Fraction I. Includes complimentary fractions for 2-5 conjugation reactions depending on conditions.

  • Type: Proteins and peptides
  • Form: 400 g in 50 mM HEPES, pH 7.6, containing 1 mM DTT.
Info GeneTexGeneTex
14785 Omicron Dr. Suite101
San Antonio, TX 78245
Customer Service: (877) 436-3839
Fax Number: (210) 677-8843
Web Site: http://www.genetex.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Human CALCA EIA (Enzyme immunoassay) Kit, Host: Rabbit ; Extraction-free from BACHEM
2. 26S Proteasome Fraction Extract from GeneTex
3. Extracti-Gel D Detergent Removing Gel AffinityPak Columns from Pierce Biotechnology, Inc.
4. Ubiquitin/Proteasome Fraction II Extract from GeneTex
5. 26S Proteasome Fraction Extract from GeneTex
6. IRS Extract from GeneTex
7. Ubiquitin Fraction I Extract from GeneTex
8. Feature Extraction Software; 2-year License for Not-For-Profit/Academic from Agilent Technologies
9. Feature Extraction Software; 1-year license for Commercial from Agilent Technologies
10. Feature Extraction Software; 1-year License for Not-For-Profit/Academic from Agilent Technologies
11. Panorama Mouse/Rat Tissue Extract Protein Array Kit from Sigma-Aldrich
... The involvement of integrins in vascular ... well-documented. The integrin family of cell adhesion ... heterodimers, composed of an a and b ... The structure and functional diversity of the ...
... of 3 siRNAs specifically targeted to your ... product. The siTrio reagent guarantees you get ... gene when used under standard conditions (100 ... optimal transfection with one of B-Bridges controls). ...
... of 3 siRNAs specifically targeted to your ... product. The siTrio reagent guarantees you get ... gene when used under standard conditions (100 ... optimal transfection with one of B-Bridges controls). ...
... has improved stability and convenience from the former ... has overcome this problem but also produced some ... the formulation of HRP conjugated antibody from lyophilized ... change, the stability of the conjugate has improved ...
Biology Products:
(Date:4/19/2017)... ALBANY, New York , April 19, 2017 /PRNewswire/ ... highly competitive, as its vendor landscape is marked by ... in the market is however held by five major ... and Safran. Together these companies accounted for nearly 61% ... majority of the leading companies in the global military ...
(Date:4/13/2017)... , April 13, 2017 According to a ... Identity Authentication, Identity Analytics, Identity Administration, and Authorization), Service, Authentication Type, Deployment ... the IAM Market is expected to grow from USD 14.30 Billion in ... Rate (CAGR) of 17.3%. ... MarketsandMarkets Logo ...
(Date:4/11/2017)... , April 11, 2017 NXT-ID, ... security technology company, announces the appointment of independent Directors Mr. ... to its Board of Directors, furthering the company,s corporate ... ... NXT-ID, we look forward to their guidance and benefiting from ...
Breaking Biology News(10 mins):
(Date:4/27/2017)... ... April 27, 2017 , ... ... Borlaug CAST Communication Award goes to Jayson Lusk, a consummate communicator who promotes ... media to advocate for science, as he explains how innovation and growth in ...
(Date:4/26/2017)... Myrtle Beach, SC (PRWEB) , ... April 26, ... ... for the mind, has teamed up with NASA to showcase the future of ... NASA’s Space Launch System (SLS) rocket and Orion spacecraft and includes a guest ...
(Date:4/26/2017)... ... April 26, 2017 , ... ... and North America this May on the following dates: , ... H. Taylor, Chairman of the Learning and Performance Institute will be the ...
(Date:4/25/2017)... ... April 25, 2017 , ... Franz ... Lisp (CL) development tools, and market leader for Semantic Graph Database ... now available within the most effective system for developing and deploying applications to ...
Breaking Biology Technology: