Navigation Links
Transcend tRNA from Promega

ProductsTranscend tRNA from Promega
Company Promega
Item Transcend tRNA
Price Inquire
Description The Transcend Non-Radioactive Translation Detection Systems allow non-radioactive detection of proteins synthesized in vitro. Using these systems, biotinylated lysine residues are incorporated into nascent proteins during translation, eliminating the need for labeling with [35-S]methionine or other radioactive amino acids. This biotinylated lysine is added to the translation reaction as a precharged epsilon-labeled biotinylated lysine-tRNA complex (Transcend tRNA) rather than a free amino acid. After SDS-PAGE and electroblotting, the biotinylated proteins can be visualized by binding either Streptavidin-Alkaline Phosphatase (Streptavidin-AP) or Streptavidin-Horseradish Peroxidase (Streptavidin-HRP), followed either by colorimetric or chemiluminescent detection. Typically, 0.5-5ng of protein can be detected by these methods within 3-4 hours after gel electrophoresis. This sensitivity is equivalent to that achieved with [35-S]methionine incorporation and autoradiographic detection 6-12 hours after gel electrophoresis.
Info PromegaPromega
2800 Woods Hollow Rd.
Madison, WI 53711-5399 USA
Customer Service: 800-356-9526
Tech Support: 1-800-356-9526
Fax Number: 800-356-1970
Web Site: http://www.promega.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Canine Pancreatic Microsomal Membranes from Promega
2. MagneGST™ Protein Purification System from Promega
3. TNT SP6 Coupled Reticulocyte Lysate System, Trial Size from Promega
4. AgarACE Enzyme from Promega
5. DNA Polymerase I from Promega
6. RQ1 RNase-Free DNase from Promega
7. AMV Reverse Transcriptase from Promega
8. PPase-2A from Promega
9. Recombinant RNasin® Ribonuclease Inhibitor from Promega
10. RNase ONE™ Ribonuclease from Promega
11. Klenow Fragment, Exonuclease Minus from Promega
...
Rabbit polyclonal to JNK1 (phospho T183 + Y185) ( Abpromise for all tested applications). entrezGeneID: 5599 SwissProtID: P45983...
Permeabilization Solution, 5X...
PCR purification kit, 100 preps...
Biology Products:
(Date:1/30/2020)... ... 30, 2020 , ... Accelera Canada , a full-service firm delivering end-to-end ... pleased to announce that the company has opened the doors of its first office ... , “We are so thrilled to now have all our partners and their ...
(Date:1/29/2020)... , ... January 29, 2020 , ... ... and in vitro toxicology testing solutions, has announced a record earnings year for ... weeks, INDIGO showed more than a twenty percent increase in revenue and a ...
(Date:1/28/2020)... OF PRUSSIA, Pa. and CAMBRIDGE, Mass. (PRWEB) , ... ... ... dedicated to solving complex problems for life sciences companies and government agencies, announced ... largest life sciences hubs in the country. Tunnell’s Cambridge office will be in ...
Breaking Biology News(10 mins):
(Date:2/21/2020)... ... February 20, 2020 , ... ... elegant chemistry. Humans have been increasingly successful in innovating ways to engineer ... evolving technologies and strategies for modern molecular biology (e.g., gene sequencing, writing, ...
(Date:2/19/2020)... ... 18, 2020 , ... Shoreline Biome, a microbiome ... level, recently unveiled a novel DNA isolation product. , Shoreline Rapid Prep is ... molecular weight (HMW) single-stranded DNA (ssDNA) in excess of 40 kB from Gram-negative ...
(Date:2/11/2020)... ... February 11, 2020 , ... ... pleased to announce the successful results from a long-term follow-up clinical study ... Inspan interspinous fixation device (INSPAN LLC). Unlike extension block design, the Inspan ...
(Date:2/5/2020)... ... February 05, 2020 , ... Rare Disease Day® ... by The National Organization for Rare Disorders (NORD), to raise awareness amongst the general ... ). The Balancing Act’s “Behind The Mystery” rare disease series will be ...
Breaking Biology Technology: