Navigation Links
Thermo Sequenase Dye Primer Manual Cycle Sequencing Kit from USB Corp.

ProductsThermo Sequenase Dye Primer Manual Cycle Sequencing Kit from USB Corp.
Company USB Corp.
Item Thermo Sequenase Dye Primer Manual Cycle Sequencing Kit
Price 
Description The Thermo Sequenase Dye Primer Manual Cycle Sequencing Kit is designed to be used with fluorescent dye-labeled primers and high-resolution fluorescence scanners. Sequencing reactions involve a termination step followed by standard slab gel electrophoresis and scanning of the gel to detect fluorescently tagged sequencing products.

The kit components have been optimized for sequencing directly from single bacterial colonies or plaques of M13 or lambda. No purification or pre-amplification is required. This kit permits rapid screening and sequencing of a large number of samples in a single working day. This kit can also be used for sequencing from 2 plasmids in yeast liquid culture. Isolation of yeast plasmids can be carried out using the USB Yeast Plasmid Isolation Kit
(PN 79220).
In all cases, the gel can be scanned without opening the glass plates, thus, allowing gel electrophoresis to be stopped at any point for analysis and then resumed if longer read lengths are needed.
Direct Sequencing
Sequence from bacterial colonies, M13 or lambda plaques. Single yeast patches on plates or small liquid culture may be sequenced by using this kit combined with the Yeast Plasmid Isolation Kit, PN 79220 from USB.
Single-Step Protocol
Sequencing reactions involve use of fluorescent-labeled primers and a termination step. Note: The choice of fluorescent dyes depends on the scanner. For instruments equipped with a
532 nm laser (e.g. Hitachis FMBIO II or III Fluorescence Imaging Systems or Molecular Dynamics Typhoon), TAMRA (Rhodamine dye) or HEX (fluorescein dye) labeled primers work well. ROX and FAM labeled primers may also be used.
Thermo Sequenase DNA Polymerase/TAP Enzyme Mix
The enzyme is a mixture of Thermo Sequenase and thermostable inorganic pyrophosphatase (TAP) cloned from the thermophile Thermoplasma acidophilum. TAP prevents pyrophosphorolysis from occurring. Pyrophosphorolysis can result in faint bands, which can affect the accuracy of base calling.
Resolve Gel Compressions with 7-deaza-dGTP Nucleotide Mixes
When DNA is synthesized with 7-deaza-dGTP instead of dGTP, weaker secondary structures are formed, thus reducing compression artifacts in sequencing gels.
Specialized Formamide Loading Dye
A special formulation of the two tracking dyes does not obscure TAMRA or HEX primers. The faster migrating dye fluoresces at a wavelength greater than 649 nm. The slower migrating dye fluoresces at a wavelength greater than 600 nm. Thus, sequences will not be obscured if TAMRA or HEX labeled primers are used.
Direct Loading of Samples on the Gel
Alcohol precipitation of the sequencing reaction products is not required. Simply load the reaction onto a standard sequencing gel.
Flexible Labeling
Components can also be used with radioactively labeled primers. However, for optimal results, a higher quality and quantity of DNA template is needed.
Important Notes:
The formulation of Thermo Sequenase DNA Polymerase in this kit necessitates the use of a glycerol tolerant DNA sequencing gel to eliminate glycerol-induced distortion of bands at approximately 350 to 600 bases beyond the primer. Information on use is included in the kits protocol. If this region is beyond your reading range, Tris-Borate-EDTA Buffer (TBE) may still be used.

Kit Components:
Thermo Sequenase with thermostable inorganic Pyrophosphatase
Reaction Buffer (concentrate)
Control DNA pUC19
Control Primer, TAMRA -40 Universal Forward Primer
ddG Termination Mix (7-deaza-dGTP)
ddA Termination Mix (7-deaza-dGTP)
ddT Termination Mix (7-deaza-dGTP)
ddC Termination Mix (7-deaza-dGTP)
Formamide Loading Dye
Protocol Book
Info USB Corp.USB Corp.
26111 Miles Road
Cleveland Ohio 44128


Call USB Corp. to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-216-245-1829
Customer Service: 216.765.5000
Fax Number: 216.464.5075
Web Site: http://www.usbweb.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Finnigan Pocket Surveyor from Thermo Scientific
2. DSQ II Single Quadrupole GC/MS from Thermo Scientific
3. LXQ High Throughput Linear Ion Trap from Thermo Scientific
4. LTQ XL Linear Ion Trap from Thermo Scientific
5. Thermo-Fast 96 PCR Plate, Non-Skirted from ABgene
6. ChromQuest is a powerful and flexible Chromatography Data System (CDS) from Thermo Scientific
7. Thermostable dUTPase from GenScript Corporation
8. TSQ Quantum Ultra from Thermo Scientific
9. LTQ XL with ETD from Thermo Scientific
10. Wellwash 4 Mk 2 from Thermo Scientific
11. Wellwash 4 Mk 2 (220-240 V) from Thermo Scientific
... signaling of the members of the GPCR ... alternative splicing--which has important implications for drug ... a classical expression profiling and at the ... forms of the gene that are expressed ...
... Alternative Splicing plays an important role ... in apoptosis and cell death. Splice variants ... activation and inhibition of defined apoptotic pathways. ... you can perform a classical expression profiling ...
... The RapidTrace SPE Workstation is a modular, ... extraction platform. Utilizing 1ml or 3ml industry ... up to 100 samples in less than ... loaded with 10 cartridges, and up to ...
... conserved form of cell suicide, which follows ... component of this process is a cascade ... enzymes participate in a series of reactions ... signals and result in the cleavage of ...
Biology Products:
(Date:1/18/2017)...  In vitro diagnostic (IVD) companies were very active ... and Kalorama Information expects that trend to continue – ... Generally, uncertainty in reimbursement and healthcare reform in ... the acquisitions landscape. Instead of looking to buy technology, ... outside of their home country and also to increase ...
(Date:1/12/2017)... Jan. 12, 2017  New research undertaken by Fit ... the future.  1,000 participants were simply asked which office technology ... we may consider standard issue.  Insights on what will ... also gathered from futurists and industry leaders including Penelope ... Canton .  Some of these findings ...
(Date:1/6/2017)... LA JOLLA, Calif. , Jan. 6, 2017 ... Phase 1 safety studies in healthy volunteers of ... CM4620, intended to treat acute pancreatitis. ... pancreas, is typically a mild disorder, but can ... to organ failure and sepsis, where extended hospital ...
Breaking Biology News(10 mins):
(Date:1/19/2017)... FRANKLIN LAKES, N.J. , Jan. 18, 2017 BD ... medical technology company, announced today that it will host a live ... 2017, at 1 p.m. (ET). The webcast can ... will be available for replay through Tuesday, January 31, 2017. ... About BD ...
(Date:1/18/2017)... to a new market research report "In situ Hybridization Market by Technique (FISH, ... Laboratories, Academic and Research Institutions) - Global Forecast to 2021" published by MarketsandMarkets, ... 557.1 Million in 2016, growing at a CAGR of 5.8%. ... ... Logo ...
(Date:1/18/2017)... , Jan. 18, 2017 Applied BioMath ... modeling to drug research and development, today announced ... President, and CEO of Applied BioMath, will present ... and Modeling (BAGIM) Meeting on Thursday January 19, ... Cambridge , MA.   Dr. Burke,s talk "Quantitative ...
(Date:1/18/2017)... ... January 18, 2017 , ... Executive ... 2017 in its continued commitment to the advancement of the clinical trials segment. ... issues related to clinical trial planning and management. , As executive talent ...
Breaking Biology Technology: