Navigation Links
Thermo Sequenase Dye Primer Manual Cycle Sequencing Kit from USB Corp.

ProductsThermo Sequenase Dye Primer Manual Cycle Sequencing Kit from USB Corp.
Company USB Corp.
Item Thermo Sequenase Dye Primer Manual Cycle Sequencing Kit
Price 
Description The Thermo Sequenase Dye Primer Manual Cycle Sequencing Kit is designed to be used with fluorescent dye-labeled primers and high-resolution fluorescence scanners. Sequencing reactions involve a termination step followed by standard slab gel electrophoresis and scanning of the gel to detect fluorescently tagged sequencing products.

The kit components have been optimized for sequencing directly from single bacterial colonies or plaques of M13 or lambda. No purification or pre-amplification is required. This kit permits rapid screening and sequencing of a large number of samples in a single working day. This kit can also be used for sequencing from 2 plasmids in yeast liquid culture. Isolation of yeast plasmids can be carried out using the USB Yeast Plasmid Isolation Kit
(PN 79220).
In all cases, the gel can be scanned without opening the glass plates, thus, allowing gel electrophoresis to be stopped at any point for analysis and then resumed if longer read lengths are needed.
Direct Sequencing
Sequence from bacterial colonies, M13 or lambda plaques. Single yeast patches on plates or small liquid culture may be sequenced by using this kit combined with the Yeast Plasmid Isolation Kit, PN 79220 from USB.
Single-Step Protocol
Sequencing reactions involve use of fluorescent-labeled primers and a termination step. Note: The choice of fluorescent dyes depends on the scanner. For instruments equipped with a
532 nm laser (e.g. Hitachis FMBIO II or III Fluorescence Imaging Systems or Molecular Dynamics Typhoon), TAMRA (Rhodamine dye) or HEX (fluorescein dye) labeled primers work well. ROX and FAM labeled primers may also be used.
Thermo Sequenase DNA Polymerase/TAP Enzyme Mix
The enzyme is a mixture of Thermo Sequenase and thermostable inorganic pyrophosphatase (TAP) cloned from the thermophile Thermoplasma acidophilum. TAP prevents pyrophosphorolysis from occurring. Pyrophosphorolysis can result in faint bands, which can affect the accuracy of base calling.
Resolve Gel Compressions with 7-deaza-dGTP Nucleotide Mixes
When DNA is synthesized with 7-deaza-dGTP instead of dGTP, weaker secondary structures are formed, thus reducing compression artifacts in sequencing gels.
Specialized Formamide Loading Dye
A special formulation of the two tracking dyes does not obscure TAMRA or HEX primers. The faster migrating dye fluoresces at a wavelength greater than 649 nm. The slower migrating dye fluoresces at a wavelength greater than 600 nm. Thus, sequences will not be obscured if TAMRA or HEX labeled primers are used.
Direct Loading of Samples on the Gel
Alcohol precipitation of the sequencing reaction products is not required. Simply load the reaction onto a standard sequencing gel.
Flexible Labeling
Components can also be used with radioactively labeled primers. However, for optimal results, a higher quality and quantity of DNA template is needed.
Important Notes:
The formulation of Thermo Sequenase DNA Polymerase in this kit necessitates the use of a glycerol tolerant DNA sequencing gel to eliminate glycerol-induced distortion of bands at approximately 350 to 600 bases beyond the primer. Information on use is included in the kits protocol. If this region is beyond your reading range, Tris-Borate-EDTA Buffer (TBE) may still be used.

Kit Components:
Thermo Sequenase with thermostable inorganic Pyrophosphatase
Reaction Buffer (concentrate)
Control DNA pUC19
Control Primer, TAMRA -40 Universal Forward Primer
ddG Termination Mix (7-deaza-dGTP)
ddA Termination Mix (7-deaza-dGTP)
ddT Termination Mix (7-deaza-dGTP)
ddC Termination Mix (7-deaza-dGTP)
Formamide Loading Dye
Protocol Book
Info USB Corp.USB Corp.
26111 Miles Road
Cleveland Ohio 44128


Call USB Corp. to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-216-245-1829
Customer Service: 216.765.5000
Fax Number: 216.464.5075
Web Site: http://www.usbweb.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Finnigan Pocket Surveyor from Thermo Scientific
2. DSQ II Single Quadrupole GC/MS from Thermo Scientific
3. LXQ High Throughput Linear Ion Trap from Thermo Scientific
4. LTQ XL Linear Ion Trap from Thermo Scientific
5. Thermo-Fast 96 PCR Plate, Non-Skirted from ABgene
6. ChromQuest is a powerful and flexible Chromatography Data System (CDS) from Thermo Scientific
7. Thermostable dUTPase from GenScript Corporation
8. TSQ Quantum Ultra from Thermo Scientific
9. LTQ XL with ETD from Thermo Scientific
10. Wellwash 4 Mk 2 from Thermo Scientific
11. Wellwash 4 Mk 2 (220-240 V) from Thermo Scientific
(Date:8/23/2017)... , Aug. 23, 2017  The general public,s help is being ... microbiome—the bacteria that live in and on the human body –and are ... The Microbiome ... the human microbiome, starting with the gut. The project's goal is to ... Photo credit: IBM ...
(Date:6/30/2017)... ARLINGTON, Va. , June 30, 2017 /PRNewswire-USNewswire/ ... a leading developer and supplier of face and ... the ATA Featured Product provider program. ... created an innovative way to monitor a driver,s ... benefit greatly from being able to detect fatigue ...
(Date:5/23/2017)...  Hunova, the first robotic gym for the rehabilitation and functional motor ... Genoa, Italy . The first 30 robots will be ... USA . The technology was developed and patented at the ... spin-off Movendo Technology thanks to a 10 million euro investment from entrepreneur ... ...
Breaking Biology News(10 mins):
(Date:10/11/2017)... , ... October 11, 2017 , ... ... gene in its endogenous context, enabling overexpression experiments and avoiding the use of ... small RNA guides is transformative for performing systematic gain-of-function studies. , This ...
(Date:10/11/2017)... INDIANAPOLIS , Oct. 11, 2017  VMS BioMarketing, a ... of a nationwide oncology Clinical Nurse Educator (CNE) network, which ... growing need for communication among health care professionals to enhance ... physicians, nurses, office staff, and other health care professionals to ... for breast cancer. ...
(Date:10/11/2017)... , ... October 11, 2017 , ... ... (FDA) has granted orphan drug designation to SBT-100, its novel anti-STAT3 (Signal Transducer ... treatment of osteosarcoma. SBT-100 is able to cross the cell membrane and bind ...
(Date:10/10/2017)... ... October 10, 2017 , ... Dr. Bob Harman, founder and CEO of ... Club. The event entitled “Stem Cells and Their Regenerative Powers,” was ... Dr. Harman, DVM, MPVM was joined by two human doctors: Peter B. Hanson, M.D., ...
Breaking Biology Technology: