Navigation Links
TargeTron Gene Knockout System from Sigma-Aldrich

ProductsTargeTron Gene Knockout System from Sigma-Aldrich
Company Sigma-Aldrich
Item TargeTron Gene Knockout System
Price 
Description The TargeTron(TM) Gene Knockout System provides optimized reagents and protocols for the rapid and specific disruption of bacterial genes by insertion of group II introns. The method exploits the retrohoming ability of group II introns and utilizes a simple PCR step to re-target the TargeTron group II intron for specific insertion into the host genome. Utility of the technology has been demonstrated for prokaryotic genetic engineering, systems biology and functional genomics approaches.

Compared to existing knockout methodologies, the TargeTron system is quite rapid, simple and robust. The process involves loading the target gene sequence into the well-validated TargeTron algorithm on the Sigma website (www.sigma-aldrich.com/targetronaccess), using one or more of the output primer sets to mutate the TargeTron intron template via PCR, ligation of the fragment into the pACD4K-C linear vector, transformation of the host, induction of gene disruption, selection by kanamycin resistance (insertion of activated marker) and screening for the desired knockout.

Gene knockout using the TargeTron system has been validated in a broad range of bacterial strains such asEscherichia coli, Staphylococcus aureus, Clostridium perfringens, Shigella flexneri, Salmonella typhimurium,andLactococcus lactis. The system may be modified for use in additional organisms.

Each TargeTron kit provides an access card and password for a specified number of designs (3EA or 10EA). The password gives the user access to the extensively validated TargeTron algorithm that predicts optimal intron insertion sites and designs primers for mutating the intron to insert into those sites. After selection of one or more desirable insertion points, the required oligos are ordered to begin laboratory procedures using the kit. Reagents in the 3EA kit are sufficient for 3 designs, 12 reactions. Reagents in the 10EA kit are sufficient for 10 designs, 40 reactions.
Info Sigma-AldrichSigma-Aldrich
Sigma-Aldrich Corp.
St. Louis, MO, USA
Customer Service: 800-325-3010
Tech Support: 800-325-5832
Fax Number: 314-771-5757
Web Site: http://www.sigmaaldrich.com/
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. R.A.P.I.D. System from Idaho Technology Inc.
2. CEQ 8800 Genetic Analysis System from Beckman Coulter
3. ProteomeLab PA 800 Protein Characterization System from Beckman Coulter
4. ProteomeLab PF 2D Protein Fractionation System from Beckman Coulter
5. pET GST Fusion System 42 plus Competent Cells from Novagen
6. pET GST Fusion System 42 from Novagen
7. pET GST Fusion System 41 plus Competent Cells from Novagen
8. pET GST Fusion System 41 from Novagen
9. Mouse Dopaminergic Neuron Differentiation Kit, 2nd Generation from R&D Systems
10. CEQ 8000 Genetic Analysis System from Beckman Coulter
11. LCMS-IT-TOF Formula Predictor System from Shimadzu
... AAIPharmas Routine Testing Laboratory (RTL) can ... over 25 years. ,,Microbial method development ... to monitor the conditions of your ... for manufactured products,,We offer a full ...
... Research Scale (gram quantities of IgY) , After ... your Antigen), we segregate the appropriate number of ... 3 such eggs are collected (usually over a ... series. This involves a total of four injections ...
... were designed for full-length gene cloning and ... identify the desired cDNA clone. The first ... "Master Plate," where each well contains plasmid ... positive well(s) by gel electrophoresis of the ...
... (Dual-Stage Quadrupole) Thermo Scientific (Part of ... analytical instrumentation, introduces the latest product innovation ... Based on the proven reliability and performance ... systems, the DSQ II incorporates the new ...
Biology Products:
(Date:6/9/2016)... , June 9, 2016  Perkotek an innovation leader in attendance control systems is ... log work hours, for employers to make sure the right employees are actually signing ... http://photos.prnewswire.com/prnh/20160609/377486LOGO ... ... ...
(Date:6/7/2016)... -- Syngrafii Inc. and San Antonio Credit Union (SACU) ... Syngrafii,s patented LongPen™ eSignature "Wet" solution into SACU,s ... in greater convenience for SACU members and operational ... document workflow and compliance requirements. Logo ... Highlights: ...
(Date:6/2/2016)... The Department of Transport Management (DOTM) of ... project, for the , Supply and Delivery of ... Infrastructure , to Decatur , ... Management Solutions. Numerous renowned international vendors participated in the tendering ... selected for the most compliant and innovative solution. The contract ...
Breaking Biology News(10 mins):
(Date:6/27/2016)... June 27, 2016 /PRNewswire/ - BIOREM Inc. (TSX-V: BRM) ("Biorem" ... by its major shareholders, Clean Technology Fund I, LP ... States based venture capital funds which together ... (on a fully diluted, as converted basis), that they ... their entire equity holdings in Biorem to TUS Holdings ...
(Date:6/27/2016)... ... June 27, 2016 , ... Cancer ... what they believe could be a new and helpful biomarker for malignant pleural ... Click here to read it now. , Biomarkers are components in the ...
(Date:6/27/2016)... ... June 27, 2016 , ... Newly created 4Sight ... solutions to the healthcare market. The company's primary focus is on new product ... marketing strategies that are necessary to help companies efficiently bring their products to ...
(Date:6/27/2016)... , June 27, 2016  Liquid Biotech ... the funding of a Sponsored Research Agreement with ... tumor cells (CTCs) from cancer patients.  The funding ... CTC levels correlate with clinical outcomes in cancer ... data will then be employed to support the ...
Breaking Biology Technology: