Navigation Links
TargeTron Gene Knockout System from Sigma-Aldrich

ProductsTargeTron Gene Knockout System from Sigma-Aldrich
Company Sigma-Aldrich
Item TargeTron Gene Knockout System
Price 
Description The TargeTron(TM) Gene Knockout System provides optimized reagents and protocols for the rapid and specific disruption of bacterial genes by insertion of group II introns. The method exploits the retrohoming ability of group II introns and utilizes a simple PCR step to re-target the TargeTron group II intron for specific insertion into the host genome. Utility of the technology has been demonstrated for prokaryotic genetic engineering, systems biology and functional genomics approaches.

Compared to existing knockout methodologies, the TargeTron system is quite rapid, simple and robust. The process involves loading the target gene sequence into the well-validated TargeTron algorithm on the Sigma website (www.sigma-aldrich.com/targetronaccess), using one or more of the output primer sets to mutate the TargeTron intron template via PCR, ligation of the fragment into the pACD4K-C linear vector, transformation of the host, induction of gene disruption, selection by kanamycin resistance (insertion of activated marker) and screening for the desired knockout.

Gene knockout using the TargeTron system has been validated in a broad range of bacterial strains such asEscherichia coli, Staphylococcus aureus, Clostridium perfringens, Shigella flexneri, Salmonella typhimurium,andLactococcus lactis. The system may be modified for use in additional organisms.

Each TargeTron kit provides an access card and password for a specified number of designs (3EA or 10EA). The password gives the user access to the extensively validated TargeTron algorithm that predicts optimal intron insertion sites and designs primers for mutating the intron to insert into those sites. After selection of one or more desirable insertion points, the required oligos are ordered to begin laboratory procedures using the kit. Reagents in the 3EA kit are sufficient for 3 designs, 12 reactions. Reagents in the 10EA kit are sufficient for 10 designs, 40 reactions.
Info Sigma-AldrichSigma-Aldrich
Sigma-Aldrich Corp.
St. Louis, MO, USA
Customer Service: 800-325-3010
Tech Support: 800-325-5832
Fax Number: 314-771-5757
Web Site: http://www.sigmaaldrich.com/
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. R.A.P.I.D. System from Idaho Technology Inc.
2. CEQ 8800 Genetic Analysis System from Beckman Coulter
3. ProteomeLab PA 800 Protein Characterization System from Beckman Coulter
4. ProteomeLab PF 2D Protein Fractionation System from Beckman Coulter
5. pET GST Fusion System 42 plus Competent Cells from Novagen
6. pET GST Fusion System 42 from Novagen
7. pET GST Fusion System 41 plus Competent Cells from Novagen
8. pET GST Fusion System 41 from Novagen
9. Mouse Dopaminergic Neuron Differentiation Kit, 2nd Generation from R&D Systems
10. CEQ 8000 Genetic Analysis System from Beckman Coulter
11. LCMS-IT-TOF Formula Predictor System from Shimadzu
... from Magnetofection technology, SilenceMag is the ... Simple, rapid and easy to use, ... toxic. Specifically designed for siRNA delivery, ... very low doses of siRNA.,SilenceMag formulation ...
... Cytological Labeling Kit provides a unique signal ... cellular targets, including cell-surface receptors and subcellular ... contains the patented ELF 97 phosphatase substrate, ... at the site of enzymatic activity ...
... Magnetofection technology, SilenceMag is the most ... rapid and easy to use, SilenceMag ... Specifically designed for siRNA delivery, SilenceMag ... low doses of siRNA.,SilenceMag formulation gives ...
... Radiochemical Purity is >95%NEN Radiolabeled Ligands\n\nReceptor-related ... continual availability of new radiolabeled ligands selected ... offers a wide range of products and ... including over 400 state-of-the-art radioligands. If you ...
Biology Products:
(Date:12/15/2014)... DUBLIN , Dec. 12, 2014 Research ... announced the addition of the "Global Facial ... http://photos.prnewswire.com/prnh/20130307/600769 ... the identification of individuals. Facial recognition system measures ... as nose, jaw edges, mouth, and the distance ...
(Date:12/11/2014)... --Research and Markets , ... ) has announced the addition of the ... their offering. One major trend ... multimodal biometric systems. Multimodal biometric systems utilize more ... and identification purposes. This helps to reduce the ...
(Date:12/11/2014)... 10, 2014  Data Sciences International (DSI), the ... a new series of digital telemetry implants to ... M series, part of the PhysioTel™ Digital platform, ... possible physiologic data when incorporating functional endpoints into ... to toxicology studies has evolved from short ECG ...
Breaking Biology News(10 mins):Global Facial Recognition Market 2015-2019: Key Vendors are 3M Cogent, Cognitec Systems, NEC and Safran Group 2Global Facial Recognition Market 2015-2019: Key Vendors are 3M Cogent, Cognitec Systems, NEC and Safran Group 3Biometrics Market in India 2015-2019: Key Vendors are 3M Cogent, NEC, Safran and Suprema 2New telemetry implants expected to change how large animal toxicology studies are conducted 2
... TX -- Researchers at Sam Houston State University and the ... from the criminal justice system in Houston to establish protocols ... by crime labs. , "This is a problem-solving project that ... tested," said Dr. William Wells, who is leading the research ...
... 2011 -- Experts agree that obesity prevention should begin before ... conclusive data, health care providers often have trouble advising parents ... study soon to be published in The Journal of ... may be an effective way to help prevent obesity. ...
... to deliver payloads of cancer-fighting drugs to tumors in ... treatment. But scientists are still at a relatively early ... Although developing nanoparticles that work as "magic bullets" ... is still the goal, the reality is that ...
Cached Biology News:Research to target untested rape kits 2Research to target untested rape kits 3Study suggests prolonged bottle feeding increases the risk of obesity 2Hitting target in cancer fight now easier with new nanoparticle platform, UCLA scientists say 2
(Date:12/15/2014)... YORK & PETACH TIKVAH, Israel ... Inc. (NASDAQ: BCLI ), a leading developer ... today that wholly-owned subsidiary Brainstorm Cell Therapeutics Ltd. was ... Israel,s Office of the Chief Scientist ... been in receipt of grant support from the Office ...
(Date:12/13/2014)... N.C. (PRWEB) December 13, 2014 CanDiag, ... Charlotte) spin-out company has changed its name to OncoTab, ... accurate representation of where we are today,” said CEO ... extended our platform’s applications beyond breast cancer diagnostics to ... a reflection of that evolution.” , Founded in 2011 ...
(Date:12/13/2014)... The “Global Synthetic Biology Market (2013-2018)”, analyses and ... challenges. , The global synthetic biology market is ... Scientific, Inc. (U.S.), DuPont (U.S.), and Royal DSM ... ~65% of the total synthetic biology market. ... @ http://www.marketsandmarkets.com/Market-Reports/synthetic-biology-market-889.html , Thermo Fisher Scientific, ...
(Date:12/13/2014)... , Dec. 12, 2014  Vaccinex ... the discovery and development of therapeutic monoclonal ... today announced publication of a manuscript entitled ... inhibits remyelination in neurodegenerative disease" in the ... of Disease*.  The publication highlights an important ...
Breaking Biology Technology:BrainStorm Receives $1,100,000 for its 8th Annual Non-Dilutive Grant from Israel's Chief Scientist Office 2BrainStorm Receives $1,100,000 for its 8th Annual Non-Dilutive Grant from Israel's Chief Scientist Office 3CanDiag Inc. Expands Product Scope Under a New Name: OncoTab, Inc. 2Synthetic Biology Market to HIt $5.6304 Billion by 2018 - New Research Report by MarketsandMakets 2Synthetic Biology Market to HIt $5.6304 Billion by 2018 - New Research Report by MarketsandMakets 3Vaccinex announces publication of preclinical studies with an anti-SEMA4D antibody in animal models of neuroinflammatory and demyelinating disease 2