|Products||Taq DNA Polymerase -Klein Taq from AppliChem GmbH|
|Item||Taq DNA Polymerase -Klein Taq|
|Description|| Klein Taq is a derivative of Taq DNA polymerase. It is a 5'-exo-minus N-terminally truncated Thermus aquaticus DNA polymerase. Expressed from a gene construct in E. coli, translation initiates at Met236, bypassing the 5'-3' exonuclease domain of the DNA polymerase-encoding gene. The enzyme reveals a highly active and even more heat-stable DNA polymerase activity in comparison with Taq DNA polymerase. The optimal range of the Mg2+ concentration for Klein Taq is broader than that for the majority of thermostable polymerases. This feature permits to optimize reaction conditions easier than for other polymerases. Repeated exposure to 98 C does not diminish the enzyme activity. Significant activity remains even after exposure to 99 C. |
The mutation rate during polymerization is twofold lower for Klein Taq as compared to the full-length Taq DNA polymerase. Klein Taq has a very low 'background' ability to extend a mismatched 3'-oligonucleotide end making it suitable for mutation analysis with mutation-specific oligonucleotides.
* The product is tested for the absence of endonucleases/ nickases and exonucleases.
*Unit definition: One unit of activity is defined as the amount of enzyme required to incorporate 10 nmoles of dNTP into acid-insoluble DNA fraction in 30 minutes at 72 C.
|Info|| AppliChem GmbH|
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