Navigation Links
Taq DNA Polymerase -Klein Taq from AppliChem GmbH

ProductsTaq DNA Polymerase -Klein Taq from AppliChem GmbH
Company AppliChem GmbH
Item Taq DNA Polymerase -Klein Taq
Price 
Description Klein Taq is a derivative of Taq DNA polymerase. It is a 5'-exo-minus N-terminally truncated Thermus aquaticus DNA polymerase. Expressed from a gene construct in E. coli, translation initiates at Met236, bypassing the 5'-3' exonuclease domain of the DNA polymerase-encoding gene. The enzyme reveals a highly active and even more heat-stable DNA polymerase activity in comparison with Taq DNA polymerase. The optimal range of the Mg2+ concentration for Klein Taq is broader than that for the majority of thermostable polymerases. This feature permits to optimize reaction conditions easier than for other polymerases. Repeated exposure to 98 C does not diminish the enzyme activity. Significant activity remains even after exposure to 99 C.

The mutation rate during polymerization is twofold lower for Klein Taq as compared to the full-length Taq DNA polymerase. Klein Taq has a very low 'background' ability to extend a mismatched 3'-oligonucleotide end making it suitable for mutation analysis with mutation-specific oligonucleotides.

* The product is tested for the absence of endonucleases/ nickases and exonucleases.

*Unit definition: One unit of activity is defined as the amount of enzyme required to incorporate 10 nmoles of dNTP into acid-insoluble DNA fraction in 30 minutes at 72 C.

Info AppliChem GmbHAppliChem GmbH
AppliChem GmbH
Ottoweg 4
D-64291 Darmstadt
Germany
Tel: +49 (0)6151 / 9357-0
Fax: +49 (0)6151 / 9357-11

AppliChem Inc.
505 Highland Avenue
Cheshire, CT 06410 U.S.A.
Tel: (800) 620-7783
Fax: (800) 823-9903

Customer Service: +49 (0)6151 / 9357-0, 1 (800) 620-7783
Fax Number: +49 (0)6151 / 9357-11, 1 (800) 823-9903
Web Site: http://www.applichem.com/
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. T4 DNA Polymerase from Roche Applied Science
2. T4 DNA Polymerase from Roche Applied Science
3. Poly A Polymerase from Invitrogen
4. iTaq DNA Polymerase, 250 U from Bio-Rad
5. iTaq DNA Polymerase, 5,000 U from Bio-Rad
6. Taq DNA Polymerase, 500 U (2 x 250 U) without dNTP from Bioneer, Inc.
7. T4 DNA Polymerase from Invitrogen
8. T4 DNA Polymerase from New England Biolabs
9. SP6 RNA Polymerase from Roche Applied Science
10. Taq DNA Polymerase (cloned) from GE Healthcare, formerly Amersham Biosciences
11. Mango-Taq DNA Polymerase from Bioline
alpha/beta-Tubulin Antibody Ship: Hot Store: -20 C...
Anti-Kusabira Orange Monoclonal Antibody Description: 100 g Research Focus: tag Storage: -20C Shipping Temperature: 4C...
TXNL2 Antibody...
Mouse monoclonal [3F8 ] to smooth muscle Myosin heavy chain I ( Abpromise for all tested applications). entrezGeneID: 4629 SwissProtID: P35749...
Biology Products:
(Date:4/17/2017)... , April 17, 2017 NXT-ID, Inc. ... company, announces the filing of its 2016 Annual Report on Form ... Exchange Commission. ... Form 10-K is available in the Investor Relations section of the ... on the SEC,s website at http://www.sec.gov . 2016 ...
(Date:4/11/2017)... -- Research and Markets has announced the addition of ... offering. ... market to grow at a CAGR of 30.37% during the period ... has been prepared based on an in-depth market analysis with inputs ... growth prospects over the coming years. The report also includes a ...
(Date:4/5/2017)... -- The Allen Institute for Cell Science today announces the ... and dynamic digital window into the human cell. The ... of deep learning to create predictive models of cell ... growing suite of powerful tools. The Allen Cell Explorer ... available resources created and shared by the Allen Institute ...
Breaking Biology News(10 mins):
(Date:10/12/2017)... ... October 12, 2017 , ... ... Rosalind™, the first-ever genomics analysis platform specifically designed for life science researchers ... honor of pioneering researcher Rosalind Franklin, who made a major contribution to ...
(Date:10/11/2017)... ... 11, 2017 , ... The CRISPR-Cas9 system has ... and avoiding the use of exogenous expression plasmids. The simplicity of programming this ... gain-of-function studies. , This complement to loss-of-function studies, such as with RNAi ...
(Date:10/11/2017)... ... October 11, 2017 , ... ComplianceOnline’s Medical Device Summit is back for its ... 2018 in San Francisco, CA. The Summit brings together current and former FDA office ... directors and government officials from around the world to address key issues in device ...
(Date:10/11/2017)... BioMarketing, a leading provider of patient support solutions, has announced ... network, which will launch this week. The VMS CNEs will ... to enhance the patient care experience by delivering peer-to-peer education ... professionals to help women who have been diagnosed and are ... ...
Breaking Biology Technology: