Navigation Links
Taq DNA Polymerase -Klein Taq from AppliChem GmbH

ProductsTaq DNA Polymerase -Klein Taq from AppliChem GmbH
Company AppliChem GmbH
Item Taq DNA Polymerase -Klein Taq
Price 
Description Klein Taq is a derivative of Taq DNA polymerase. It is a 5'-exo-minus N-terminally truncated Thermus aquaticus DNA polymerase. Expressed from a gene construct in E. coli, translation initiates at Met236, bypassing the 5'-3' exonuclease domain of the DNA polymerase-encoding gene. The enzyme reveals a highly active and even more heat-stable DNA polymerase activity in comparison with Taq DNA polymerase. The optimal range of the Mg2+ concentration for Klein Taq is broader than that for the majority of thermostable polymerases. This feature permits to optimize reaction conditions easier than for other polymerases. Repeated exposure to 98 C does not diminish the enzyme activity. Significant activity remains even after exposure to 99 C.

The mutation rate during polymerization is twofold lower for Klein Taq as compared to the full-length Taq DNA polymerase. Klein Taq has a very low 'background' ability to extend a mismatched 3'-oligonucleotide end making it suitable for mutation analysis with mutation-specific oligonucleotides.

* The product is tested for the absence of endonucleases/ nickases and exonucleases.

*Unit definition: One unit of activity is defined as the amount of enzyme required to incorporate 10 nmoles of dNTP into acid-insoluble DNA fraction in 30 minutes at 72 C.

Info AppliChem GmbHAppliChem GmbH
AppliChem GmbH
Ottoweg 4
D-64291 Darmstadt
Germany
Tel: +49 (0)6151 / 9357-0
Fax: +49 (0)6151 / 9357-11

AppliChem Inc.
505 Highland Avenue
Cheshire, CT 06410 U.S.A.
Tel: (800) 620-7783
Fax: (800) 823-9903

Customer Service: +49 (0)6151 / 9357-0, 1 (800) 620-7783
Fax Number: +49 (0)6151 / 9357-11, 1 (800) 823-9903
Web Site: http://www.applichem.com/
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. T4 DNA Polymerase from Roche Applied Science
2. T4 DNA Polymerase from Roche Applied Science
3. Poly A Polymerase from Invitrogen
4. iTaq DNA Polymerase, 250 U from Bio-Rad
5. iTaq DNA Polymerase, 5,000 U from Bio-Rad
6. Taq DNA Polymerase, 500 U (2 x 250 U) without dNTP from Bioneer, Inc.
7. T4 DNA Polymerase from Invitrogen
8. T4 DNA Polymerase from New England Biolabs
9. SP6 RNA Polymerase from Roche Applied Science
10. Taq DNA Polymerase (cloned) from GE Healthcare, formerly Amersham Biosciences
11. Mango-Taq DNA Polymerase from Bioline
Liquid. 50 μg/ml in PBS, pH 7.4. PROTECT FROM LIGHT. A convenient form of propidium iodide useful for flow cytometry studies. RTECS SF7949600, CAS 25535-16-4, M.W. 668.4. ...
... The Poly-Caspases FLICA Apoptosis Detection Kit contains ... a carboxyfluorescein (FAM) derivative of valylalanylaspartic acid ... of caspase activity. When added to ... probe enters each cell and covalently binds ...
Lyophilized solid. Suitable for evaluating functional activity of human complement components and for other research requiring a high level of hemolytic activity. ...
... system, 220-240 V, uses floating pin printing ... lipids, and other biomolecules onto a variety ... can generate high-density arrays with greater than ... than 57,000 spots per membrane with a ...
Biology Products:
(Date:6/14/2017)...  IBM (NYSE: IBM ) is introducing several innovative ... to developing collaboration between startups and global businesses, taking place ... the event, nine startups will showcase the solutions they have ... France is one ... a 30 percent increase in the number of startups created ...
(Date:5/16/2017)...   Bridge Patient Portal , an enterprise ... EMR Systems , an electronic medical record solutions ... established a partnership to build an interface between ... Centricity™ products, including Centricity Practice Solution (CPS), Centricity ... new integrations will allow healthcare delivery networks using ...
(Date:4/19/2017)... , April 19, 2017 The ... vendor landscape is marked by the presence of several ... however held by five major players - 3M Cogent, ... companies accounted for nearly 61% of the global military ... companies in the global military biometrics market boast global ...
Breaking Biology News(10 mins):
(Date:10/12/2017)... ... October 12, 2017 , ... ... prospective clinical study that demonstrates the accuracy of the FebriDx® test, a ... significant acute bacterial and viral respiratory tract infections by testing the body’s ...
(Date:10/12/2017)... ... , ... AMRI, a global contract research, development and manufacturing ... and quality of life, will now be offering its impurity solutions as a ... requirements for all new drug products, including the finalization of ICH M7 earlier ...
(Date:10/11/2017)... N.C. (PRWEB) , ... October 11, 2017 , ... ... ARCS® Foundation President Andi Purple announced Dr. Suneel I. Sheikh, the co-founder, ... ( ASTER Labs ), Inc. has been selected for membership in ARCS ...
(Date:10/11/2017)... ... October 11, 2017 , ... Personal eye wash is a basic first aid supply for ... time. So which eye do you rinse first if a dangerous substance enters both eyes? ... Eye Wash with its unique dual eye piece. , “Whether its dirt and debris, ...
Breaking Biology Technology: