Navigation Links
TURBO DNase (2 U/l) from Ambion

ProductsTURBO DNase (2 U/l) from Ambion
Company Ambion
Item TURBO DNase (2 U/l)
Price $75.00
Description TURBO DNase (patent pending) was developed using a protein engineering approach that introduced amino acid changes into the DNA binding pocket of wild-type DNase I. These changes markedly increase the affinity of the protein for DNA. The result is a versatile enzyme that has a 6-fold lower Km for DNA, and an ability to maintain at least 50% of peak activity in solutions approaching 200 mM monovalent salt, even when the DNA concentration is in the nanomolar (nM) range. The flexibility of TURBO DNase activity is particularly evident when comparing the efficiency of DNA removal from in vitro transcription buffer, as shown in Figure 1. In this case, TURBO DNase reduces the input 50 kb lambda DNA to digested fragments that are roughly 100 times smaller than those treated with conventional DNase I. This qualitative result can be more precisely quantified using real-time RT-PCR (Table 2). When in vitro transcription reactions are treated with either DNase I or TURBO DNase, TURBO DNase removes 63X more of the input plasmid DNA tempate than the wild-type enzyme. As a result, TURBO DNase is the enzyme of choice for eliminating plasmid DNA templates from transcription reactions, particularly for the synthesis of RNA standards used in RT-PCR and generating RNA for microinjection and transfection experiments. The proficiency of TURBO DNase in binding very low concentrations of DNA means that the enzyme is particularly effective in removing trace quantities of DNA contamination. This becomes important for complete removal of DNA from a sample, since the cleavable DNA substrate is reduced as the DNase reaction proceeds. TURBO DNase thus has a functional advantage over wild-type DNase due to its superior affinity for DNA. This is best exploited in RT-PCR applications, where even a few copies of DNA can lead to a false positive outcome by PCR. As shown in Table 1, TURBO DNase is more effective than wild-type DNase in reducing DNA detection by PCR.
Info AmbionAmbion
2130 Woodward
Austin, TX 78744
USA

Call Ambion to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-512-795-4221
Customer Service: 512-651-0200
Fax Number: 512-651-0201
Web Site: http://www.ambion.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. TURBO DNA-free™ from Ambion
2. DNase I (RNase-free) (2 U/l) from Ambion
3. RQ1 RNase-Free DNase from Promega
4. DNase I, RNase-free; special quality for molecular biology from Roche Applied Science
5. DNase I from Invitrogen
6. DNase I, ds Qualified from Novagen
7. DNase I Amplification Grade from Invitrogen
8. RNase, DNase-free from Roche Applied Science
9. Rabbit Anti-Human DNase II Polyclonal Antibody, Unconjugated from Calbiochem
10. UltraPure DNase/RNase-Free Distilled Water from Invitrogen
11. UltraPure DNase/RNase-Free Distilled Water from Invitrogen
... and Protein G bind specifically to Fc regions of ...ein G conjugates are commonly used as affinity ads... immunoglobulin subtypes from serum, hybridoma asc... biological fluids. These reagents are also common...
CORNING PETRI DISH, 100 X 20 MM, PS, ULTRA LOW BINDING, STERILE from Sigma-Aldrich
...se-iT™ One-Step Kit is the ultimate in conven...It has been specifically designed to streamline th...it combines the unique Reverse-iT™ Blend of R...erase to provide a highly sensitive, reproducible ...
... and Protein G bind specifically to Fc regions of ...ein G conjugates are commonly used as affinity ads... immunoglobulin subtypes from serum, hybridoma asc... biological fluids. These reagents are also common...
Biology Products:
(Date:11/24/2009)... , Rocket science is opening new doors to underst... affect the hearing of a marine mammal or if they...rial sized X-ray scanners that NASA uses to detect...rockets is now allowing scientists to peek inside ... detailed three-dimensional replicas of a whale,s ...
(Date:11/24/2009)... , The atomic-level action of a remarkable class ...y researchers with the Lawrence Berkeley National ... protein crystallography beamline at the Advanced ...tal roles in gene expression and replication, and ... as well as infectious agents, such as the human p...
(Date:11/24/2009)... , BATON ROUGE Antarctica has long held secret... and until recently, there has been very little in...d beneath miles of ice for millions of years. Now,...luding LSU have been funded to the tune of $10 mi...or NSF, to get to the bottom of things literally....
Breaking Biology News(10 mins):Rocket science leads to new whale discovery 2Atomic-level snapshot catches protein motor in action 2Atomic-level snapshot catches protein motor in action 3Atomic-level snapshot catches protein motor in action 4LSU gets to the bottom of things -- in Antarctica 2Judge Rules on SEIU Lawsuit 50208 1Judge Rules on SEIU Lawsuit 50208 2National Expert on Prescription Drug Abuse Addicted Physicians Available for Media Interviews From Hazelden 50206 1National Autism Association Teams Up With 12 Organizations to End Abusive Restraint and Seclusion in Schools 50203 1National Autism Association Teams Up With 12 Organizations to End Abusive Restraint and Seclusion in Schools 50203 2
...ntists have now found that changes in a single gen...of fish living in the wild. , The surprising resu...rnal Science, bring new data to long-standing deba.... , "Our motivation is to try to understand how ne...eneticist David M. Kingsley, a Howard Hughes Medic...
...imals evolve, researchers sometimes need to put on...earn how their theory compares to the real world. ...ine study published in the March 25 issue of Scien...eriments suggest. , In a diverse group of fish cal...same genetic trick time and again to allow freshwa...
... their pollinators have resulted in a spectacular ...s such as bees, flies or butterflies to visit and ...evolved intriguing mechanisms and attractants, of ...esearchers discovered that nectars of flowers poll...of amino acids. Recent experiments have shown that...
Other Biology News:Researchers trace evolution to relatively simple genetic changes 2Researchers trace evolution to relatively simple genetic changes 3Researchers trace evolution to relatively simple genetic changes 4Researchers trace evolution to relatively simple genetic changes 5Same mutation aided evolution in many fish species, Stanford study finds 2Same mutation aided evolution in many fish species, Stanford study finds 3
(Date:11/24/2009)... Ambassador to also Visit Detroi...2009 -- , , ,Who: David Appia, French Ambassador f...of the Invest in France Agency, , , , ,What: Amba...scuss recent pro-business initiatives adopted by F... academic landscape for innovation and development...
(Date:11/24/2009)... , SANTA CRUZ, Calif., Nov. 24 /PRNewswire/ ...io Career Center, the nation,s leading online job ...als. ,, Northwestern and Columbia University ar...ajor life science training Institutions. ,, The... post-graduates in the life sciences. While there ...
(Date:11/24/2009)... , REYKJAVIK, Iceland, November.... (Nasdaq: DCGN ) today announced that it has rece...ing in the company,s common stock will be,suspende...e filed with the,Securities and Exchange Commissio...sting on Nasdaq, unless the company files an appea...
(Date:11/24/2009)... Health care employment grew b...e only one to show consistent growth during the U....tals remain in trouble, and health systems are com...nnel, instead of just administrators. , ... care employment continued growing in October desp...
Breaking Biology Technology:Two Major Universities Join Nation's Leading Postgrad Bio Job Board 2deCODE Receives Delisting Notice From Nasdaq, Plans to Appeal 2deCODE Receives Delisting Notice From Nasdaq, Plans to Appeal 3The MedZilla Report for October 2009 - Health Care Employment Grows Again in October Even As Clinics, Specialty Centers Close 2The MedZilla Report for October 2009 - Health Care Employment Grows Again in October Even As Clinics, Specialty Centers Close 3The MedZilla Report for October 2009 - Health Care Employment Grows Again in October Even As Clinics, Specialty Centers Close 4