Navigation Links
TURBO DNase (2 U/l) from Ambion

ProductsTURBO DNase (2 U/l) from Ambion
Company Ambion
Item TURBO DNase (2 U/l)
Price $75.00
Description TURBO DNase (patent pending) was developed using a protein engineering approach that introduced amino acid changes into the DNA binding pocket of wild-type DNase I. These changes markedly increase the affinity of the protein for DNA. The result is a versatile enzyme that has a 6-fold lower Km for DNA, and an ability to maintain at least 50% of peak activity in solutions approaching 200 mM monovalent salt, even when the DNA concentration is in the nanomolar (nM) range. The flexibility of TURBO DNase activity is particularly evident when comparing the efficiency of DNA removal from in vitro transcription buffer, as shown in Figure 1. In this case, TURBO DNase reduces the input 50 kb lambda DNA to digested fragments that are roughly 100 times smaller than those treated with conventional DNase I. This qualitative result can be more precisely quantified using real-time RT-PCR (Table 2). When in vitro transcription reactions are treated with either DNase I or TURBO DNase, TURBO DNase removes 63X more of the input plasmid DNA tempate than the wild-type enzyme. As a result, TURBO DNase is the enzyme of choice for eliminating plasmid DNA templates from transcription reactions, particularly for the synthesis of RNA standards used in RT-PCR and generating RNA for microinjection and transfection experiments. The proficiency of TURBO DNase in binding very low concentrations of DNA means that the enzyme is particularly effective in removing trace quantities of DNA contamination. This becomes important for complete removal of DNA from a sample, since the cleavable DNA substrate is reduced as the DNase reaction proceeds. TURBO DNase thus has a functional advantage over wild-type DNase due to its superior affinity for DNA. This is best exploited in RT-PCR applications, where even a few copies of DNA can lead to a false positive outcome by PCR. As shown in Table 1, TURBO DNase is more effective than wild-type DNase in reducing DNA detection by PCR.
Info AmbionAmbion
2130 Woodward
Austin, TX 78744
USA

Call Ambion to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-512-795-4221
Customer Service: 512-651-0200
Fax Number: 512-651-0201
Web Site: http://www.ambion.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. TURBO DNA-free™ from Ambion
2. DNase I (RNase-free) (2 U/l) from Ambion
3. RQ1 RNase-Free DNase from Promega
4. DNase I, RNase-free; special quality for molecular biology from Roche Applied Science
5. DNase I from Invitrogen
6. DNase I, ds Qualified from Novagen
7. DNase I Amplification Grade from Invitrogen
8. RNase, DNase-free from Roche Applied Science
9. Rabbit Anti-Human DNase II Polyclonal Antibody, Unconjugated from Calbiochem
10. UltraPure DNase/RNase-Free Distilled Water from Invitrogen
11. UltraPure DNase/RNase-Free Distilled Water from Invitrogen
... The Model 1575 immunowash microplate washer ... microplates that have flat-, U-, or V-bottom ... of horizontal and vertical needle positions to ... washing, crosswise aspiration, and overflow washing. It ...
... compact benchtop and stackable multi-function incubator. ... a large display screen to accurately ... circulation inside to evenly distribute temperature ... easily calibrated from the microprocessor controller. ...
... specifically designed as a high tech shaker ... mass compensation allows high shaking speed even ... and, therefore, faster growth and higher cell ... when high reproducibility is critical ...
...
Biology Products:
(Date:5/14/2015)... 14, 2015  Verificient Technologies, Inc., a ... online remote proctoring, announced a new alliance ... and creator of the Canvas Learning Management ... companies will benefit from the seamless integration ... As a fully integrated multifactor biometrics behavioral ...
(Date:5/10/2015)... Sweden , May 11, 2015 ... fingerprint sensors FPC1025 and FPC1155 from the distributor World Peace ... of FPC,s distributors in Asia . Deliveries ... sensors will be used by smartphone manufacturers in ... included in the communicated revenue guidance of + 1 000 MSEK ...
(Date:5/5/2015)... OXFORD, Conn. , May 5, 2015, NXT-ID, ... biometric authentication company focused on the growing mobile commerce market, ... the Company,s Chief Technology Officer, is presenting  at CARTES SECURE ... May 5-7, 2015. The three-day conference ... session David is speaking in is themed Global Fraud: ...
Breaking Biology News(10 mins):Verificient and Instructure Announce Alliance Partnership to Provide Online Proctoring for K-12 and Higher Education Institutions 2Fingerprint Cards Receives Touch Fingerprint Sensor Order of SEK 235 Million 2NXT-ID, Inc.'s CTO, David Tunnell, Presents at CARTES SECURE CONNEXIONS AMERICA 2015 Today in Washington DC. 2NXT-ID, Inc.'s CTO, David Tunnell, Presents at CARTES SECURE CONNEXIONS AMERICA 2015 Today in Washington DC. 3
... Molecular machines can be found everywhere in nature, for ... develop artificial molecular machines, scientists need to understand the ... scale (a nanometer is a billionth of a meter). ... at the University of California, Riverside studied a class ...
... TEMPE, Ariz. Arizona State University,s expanding ... and science has led to formation of the ... of Regents recently approved establishment of the center, ... in electrical engineering, physics, materials science and engineering, ...
... health professionals working in the global informatics community, ... for informatics professionals. The Standards Standard provides a ... technology experts who require a reliable periodic update ... major biomedical and healthcare standards- setting organizations. Its ...
Cached Biology News:Would a molecular horse trot, pace or glide across a surface? 2Would a molecular horse trot, pace or glide across a surface? 3Focus on photonics 2Global standards for informatics professionals summed up in new B2P online publication 2
(Date:5/30/2015)... May 30, 2015  Caris Life Sciences® today ... in which researchers identified biomarker changes, including key ... tumors, indicating changes in response to therapy as ... was a subset of a larger study analyzing ... the cancer. These data utilizing Caris Molecular Intelligence®, ...
(Date:5/29/2015)... CA (PRWEB) May 29, 2015 ... addressing multi-drug-resistant organisms (MDROs), announced that initial feasibility ... same-shift bacterial detection and susceptibility testing from positive ... on May 31, 2015 in New Orleans, ... class of molecular diagnostics that can quickly detect ...
(Date:5/29/2015)... 2015 Delcath Systems, Inc. (NASDAQ: ... company focused on oncology with an emphasis on ... announces that results of a large, European single-center ... with hepatic metastases with the Delcath Hepatic CHEMOSAT ... on-line abstract at the American Society of Clinical ...
(Date:5/29/2015)... 2015 Research and Markets ( http://www.researchandmarkets.com/research/7bf7ff/cytogenetics ... new report "Cytogenetics - Technologies, Markets and ... report deals with cytogenetics in a broader sense ... the chromosome structure and identify abnormalities related to ... is also referred to as molecular cytogenetics. The ...
Breaking Biology Technology:Caris Life Sciences Paired Tumor Data Identifies Key Driver Mutations and Confirms Tumor Heterogeneity in High-Grade Glioma Samples 2Caris Life Sciences Paired Tumor Data Identifies Key Driver Mutations and Confirms Tumor Heterogeneity in High-Grade Glioma Samples 3Caris Life Sciences Paired Tumor Data Identifies Key Driver Mutations and Confirms Tumor Heterogeneity in High-Grade Glioma Samples 4Data Highlighting GeneWEAVE’s Smarticles™ Technology to be Presented at the 115th General Meeting of the American Society for Microbiology (asm2015) 2Data Highlighting GeneWEAVE’s Smarticles™ Technology to be Presented at the 115th General Meeting of the American Society for Microbiology (asm2015) 3Positive Data With Delcath's CHEMOSAT Highlighted At ASCO 2015 2Positive Data With Delcath's CHEMOSAT Highlighted At ASCO 2015 3Positive Data With Delcath's CHEMOSAT Highlighted At ASCO 2015 4Positive Data With Delcath's CHEMOSAT Highlighted At ASCO 2015 5Global Cytogenetics Market Report 2015-2024 - Technologies, Markets and Companies 2Global Cytogenetics Market Report 2015-2024 - Technologies, Markets and Companies 3