Navigation Links
TURBO DNase (2 U/l) from Ambion

ProductsTURBO DNase (2 U/l) from Ambion
Company Ambion
Item TURBO DNase (2 U/l)
Price $75.00
Description TURBO DNase (patent pending) was developed using a protein engineering approach that introduced amino acid changes into the DNA binding pocket of wild-type DNase I. These changes markedly increase the affinity of the protein for DNA. The result is a versatile enzyme that has a 6-fold lower Km for DNA, and an ability to maintain at least 50% of peak activity in solutions approaching 200 mM monovalent salt, even when the DNA concentration is in the nanomolar (nM) range. The flexibility of TURBO DNase activity is particularly evident when comparing the efficiency of DNA removal from in vitro transcription buffer, as shown in Figure 1. In this case, TURBO DNase reduces the input 50 kb lambda DNA to digested fragments that are roughly 100 times smaller than those treated with conventional DNase I. This qualitative result can be more precisely quantified using real-time RT-PCR (Table 2). When in vitro transcription reactions are treated with either DNase I or TURBO DNase, TURBO DNase removes 63X more of the input plasmid DNA tempate than the wild-type enzyme. As a result, TURBO DNase is the enzyme of choice for eliminating plasmid DNA templates from transcription reactions, particularly for the synthesis of RNA standards used in RT-PCR and generating RNA for microinjection and transfection experiments. The proficiency of TURBO DNase in binding very low concentrations of DNA means that the enzyme is particularly effective in removing trace quantities of DNA contamination. This becomes important for complete removal of DNA from a sample, since the cleavable DNA substrate is reduced as the DNase reaction proceeds. TURBO DNase thus has a functional advantage over wild-type DNase due to its superior affinity for DNA. This is best exploited in RT-PCR applications, where even a few copies of DNA can lead to a false positive outcome by PCR. As shown in Table 1, TURBO DNase is more effective than wild-type DNase in reducing DNA detection by PCR.
Info AmbionAmbion
2130 Woodward
Austin, TX 78744

Call Ambion to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-512-795-4221
Customer Service: 512-651-0200
Fax Number: 512-651-0201
Web Site:
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. TURBO DNA-free™ from Ambion
2. DNase I (RNase-free) (2 U/l) from Ambion
3. RQ1 RNase-Free DNase from Promega
4. DNase I, RNase-free; special quality for molecular biology from Roche Applied Science
5. DNase I from Invitrogen
6. DNase I, ds Qualified from Novagen
7. DNase I Amplification Grade from Invitrogen
8. RNase, DNase-free from Roche Applied Science
9. Rabbit Anti-Human DNase II Polyclonal Antibody, Unconjugated from Calbiochem
10. UltraPure DNase/RNase-Free Distilled Water from Invitrogen
11. UltraPure DNase/RNase-Free Distilled Water from Invitrogen
assays for phosphatase, lipase, nucleoside triphosphatases (ATPase, GTPase etc). HTS and follow up studies for phosphatase inhibitors. Quantitation of phosphate content in protein, lipids, and DNA....
One-step, microplate or cuvet, colorimetric, detection range 0.1 mg/dL to 3 mg/dL. Procedure: 10 min....
Human DCIR/CLEC4A Phycoerythrin MAb (Clone 216110)...
Contains 100 positive controls for effective screening of HDAC inhibitors by fluorometric method....
Biology Products:
(Date:11/18/2015)... 18, 2015  As new scientific discoveries deepen our ... other healthcare providers face challenges in better using that ... In addition, as more children continue to survive pediatric ... and old age. John M. Maris, M.D ... of Philadelphia (CHOP) . --> John ...
(Date:11/17/2015)... SOUTH EASTON, Mass. , Nov. 17, 2015 /PRNewswire/ ... "Company"), a leader in the development and sale of ... to the worldwide life sciences industry, today announced it ... closing of its $5 million Private Placement (the "Offering"), ... Offering to $4,025,000.  One or more additional closings are ...
(Date:11/11/2015)... 2015   MedNet Solutions , an innovative SaaS-based eClinical ... is pleased to announce that it will be a Sponsor ... event, to be held November 17-19 in Hamburg ... demonstrations of iMedNet , MedNet,s easy-to-use, proven ... has been able to deliver time and cost savings ...
Breaking Biology News(10 mins):
(Date:11/25/2015)... 2015  PharmAthene, Inc. (NYSE MKT: PIP) announced  today ... rights plan (Rights Plan) in an effort to preserve ... under Section 382 of the Internal Revenue Code (Code). ... use of its NOLs could be substantially limited if ... Section 382 of the Code. In general, an ownership ...
(Date:11/25/2015)... , Nov. 25, 2015  Neurocrine Biosciences, Inc. (Nasdaq: ... , President and CEO of Neurocrine Biosciences, will be ... in New York . ... the website approximately 5 minutes prior to the presentation ... of the presentation will be available on the website ...
(Date:11/25/2015)... 25, 2015 The Global ... a professional and in-depth study on the current ... (Logo: ) , The ... including definitions, classifications, applications and industry chain structure. ... international markets including development trends, competitive landscape analysis, ...
(Date:11/24/2015)... , Nov. 24, 2015 Cepheid (NASDAQ: ... be speaking at the following conference, and invited investors ... York, NY      Tuesday, December 1, 2015 at ... York, NY      Tuesday, December 1, 2015 at ... Healthcare Conference, New York, NY ...
Breaking Biology Technology: