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TURBO DNase (2 U/l) from Ambion

ProductsTURBO DNase (2 U/l) from Ambion
Company Ambion
Item TURBO DNase (2 U/l)
Price $75.00
Description TURBO DNase (patent pending) was developed using a protein engineering approach that introduced amino acid changes into the DNA binding pocket of wild-type DNase I. These changes markedly increase the affinity of the protein for DNA. The result is a versatile enzyme that has a 6-fold lower Km for DNA, and an ability to maintain at least 50% of peak activity in solutions approaching 200 mM monovalent salt, even when the DNA concentration is in the nanomolar (nM) range. The flexibility of TURBO DNase activity is particularly evident when comparing the efficiency of DNA removal from in vitro transcription buffer, as shown in Figure 1. In this case, TURBO DNase reduces the input 50 kb lambda DNA to digested fragments that are roughly 100 times smaller than those treated with conventional DNase I. This qualitative result can be more precisely quantified using real-time RT-PCR (Table 2). When in vitro transcription reactions are treated with either DNase I or TURBO DNase, TURBO DNase removes 63X more of the input plasmid DNA tempate than the wild-type enzyme. As a result, TURBO DNase is the enzyme of choice for eliminating plasmid DNA templates from transcription reactions, particularly for the synthesis of RNA standards used in RT-PCR and generating RNA for microinjection and transfection experiments. The proficiency of TURBO DNase in binding very low concentrations of DNA means that the enzyme is particularly effective in removing trace quantities of DNA contamination. This becomes important for complete removal of DNA from a sample, since the cleavable DNA substrate is reduced as the DNase reaction proceeds. TURBO DNase thus has a functional advantage over wild-type DNase due to its superior affinity for DNA. This is best exploited in RT-PCR applications, where even a few copies of DNA can lead to a false positive outcome by PCR. As shown in Table 1, TURBO DNase is more effective than wild-type DNase in reducing DNA detection by PCR.
Info AmbionAmbion
2130 Woodward
Austin, TX 78744
USA

Call Ambion to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-512-795-4221
Customer Service: 512-651-0200
Fax Number: 512-651-0201
Web Site: http://www.ambion.com
NOTE: Price information is approximate list price and actual prices may vary.

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