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T4 Polynucleotide Kinase from EPICENTRE Biotechnologies

ProductsT4 Polynucleotide Kinase from EPICENTRE Biotechnologies
Company EPICENTRE Biotechnologies
Item T4 Polynucleotide Kinase
Price $95.00
Description T4 Polynucleotide Kinase (PNK) catalyzes the transfer of the gamma-phosphate from ATP to the 5'-hydroxyl of single- and double-stranded DNA, RNA, and nucleoside 3'-monophosphates. The enzyme also removes the 3'-phosphate from 3'-phosphoryl polynucleotides, deoxyribonucleoside 3'-monophosphates, and deoxyribonucleoside 3', 5'-diphosphates to form a 3'-hydroxyl group. Storage Buffer: 50% glycerol containing 50 mM Tris-HCI, pH 7.5, 0.1 M NaCI, 0.1 mM EDTA, 0.1% Triton X-100, and 1 mM DTT. T4 PNK 10X Reaction Buffer: 330 mM Tris-acetate, pH 7.8, 660 mM potassium acetate, 100 mM magnesium acetate, and 5 mM DTT. A 10 mM solution of ATP for non-isotopic applications is available separately.
Info EPICENTRE BiotechnologiesEPICENTRE Biotechnologies
726 Post Road
Madison, WI 53713
Customer Service: 800-284-8474
Tech Support: 800-284-8474
Fax Number: 608-442-6114
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