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Superfusion System, Raiteri's Method from

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Item Superfusion System, Raiteri's Method
Description Neurotransmitters are stored within neurons in vesicles called synaptosomes. Synaptosomes are concentrated in the synaptic bouton. Synaptosomes may be centrifuged and isolated from other cellular debris. Abnormalites in neurotransmitter release have been proposed in many pathological condtions. Conditions of release may be studied in isolated synaptosome preparations.The Superfusion System of Raiteri is designed to enable a variety of methods of studying isolated synaptosome prepartions. Raiteri's labs have generated over 200 peer reviewed publications with this instrument. The system would also support multiple brain slice prepartions as well. The Superfusion System consists of 12 parallel open superfusion chambers with 12 upper reservoirs, all thermally stable with the aid of a water jacket with circulation. Warmed, oxygenated, media as desired can be concomitantly delivered from the reservoirs to the superfudsion chambers. Synaptosomes are accomodated as very thin layeers on microporous filters placed on glass filter supports. Synaptosome or slice superfusion is provided by a multichanel peristlaltic pump and superfusate samples are directly collected into scintallation vials for analysis.
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