|Products||Smac/DIABLO, Human, Recombinant, E. coli from Calbiochem|
|Item||Smac/DIABLO, Human, Recombinant, E. coli|
Second Mitochondria Derived Activator of Caspase/DIABLO
Liquid. In 100 mM KCl, 20 mM HEPES, pH 7.5 AVOID FREEZE/THAW CYCLES. Amino acid residues 56 - 239 of human Smac/DIABLO with a carboxy terminal His•Tag® sequence was expressed in and purified from E. coli. Smac/DIABLO is released along with cytochrome c during apoptosis and promotes caspase activation through the neutralization of IAPs, including XIAP (Cat. No. PF137). Biological activity: IC50~500-1500 nM to reverse the inhibition of XIAP (500 nM) on Z-DEVD-AFC (Cat. No. 264150) cleavage in activated cell extracts. Purity: ≥95% by SDS-PAGE. M.W. 22,000.
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