Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Thymus from OriGene Technologies

Navigation Links

Biology News Medicine News Biology Products Medicine Products Biology Definition Medicine Definition Biology Technology Medicine Technology Biology Dictionary Medicine Dictionary

HOME >> BIOLOGY >> PRODUCTS

Products

Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Thymus from OriGene Technologies

Company

OriGene Technologies

Item

Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Thymus

Price

$925.00

Description

The Rapid-Screen cDNA Library Panels were designed for full-length gene cloning and require just three sets of PCRs to identify the desired cDNA clone. The first PCR analysis is performed in a 96-well "Master Plate," where each well contains plasmid DNA from 5,000 clones. Having identified the positive well(s) by gel electrophoresis of the reaction products, the second PCR analysis is carried out in the corresponding 96-well "Sub-Plate(s)," which contains all 5,000 clones from the positive well of the Master Plate arrayed at only 50 clones per well. Since this plate contains glycerol stocks of E. coli instead of plasmid DNA, the cells can be diluted and plated out once a positive well(s) has been identified. Analysis of 96 single colonies by PCR leads to the identification of the positive clone from the Sub-Plate well. To ensure that a clone detected in a specific well in the Master Plate will be represented in the appropriate Sub-Plate, the Sub- Plates were not derived by dilution of individual wells from the Master Plate (a process which may lose specific clones). Instead, the Sub-Plates were generated first and the wells from each Sub-Plate were then pooled to generate the Master Plate. To guarantee that clones from different wells in the Master Plate were each independently-derived, the Sub-Plates were generated with primary transformants that had not been amplified.

Info

OriGene Technologies
6 Taft Court, Suite 100
Rockville, MD 20850
USA

Call OriGene Technologies to buy products (US and Canada only; other locations use numbers below)

1-866-592-4179

Customer Service: 1-888-267-4436 (U.S. only) / 301-340-3188
Fax Number: 301-340-9254
Web Site: http://www.origene.com

NOTE: Price information is approximate list price and actual prices may vary.

A convenience pack containing one each of the most popular sizes of Cat. No. 4980 flask. Designed for the low volume user, a case contains one each of five sizes: 50mL, 125mL, 250mL, 500mL, and 1L....

...

Rabbit polyclonal to YANK2 ( Abpromise for all tested applications). Antigen: A KLH conjugated synthetic peptide selected within aa 300~400 (C terminal region) of human YANK2. Entrez Gene ID:...

Biology Products:

Pyrex brand narrow-mouth graduated erlenmeyer flask from Sigma-Aldrich Oligonucleotides from Thermo Scientific LS Columns from Miltenyi Biotec Rabbit Anti-Human YANK2 Polyclonal Antibody, Unconjugated from Abcam

(Date:4/1/2019)... ... ... Stay on top of current hot topics through free webinars presented by ... is free, so be sure to register today to save your place! Participate in ... see our upcoming webinars: , CLINICAL TRIALS , April 2 – They’re Talking. Are ...

(Date:3/29/2019)... ... March 29, 2019 , ... RoosterBio, Inc, a leading ... Society for Biomaterials Annual Meeting & Exposition on April 3-6, 2019 in Seattle, ... healthcare, governmental and business professionals in all aspects of the biomaterial science field ...

(Date:3/26/2019)... ... March 26, 2019 , ... ... the four recipients of the Society's 2019 Outstanding Achievement Award, Outstanding New Investigator ... be presented at the ASGCT 22nd Annual Meeting . , Outstanding Achievement ...

Breaking Biology News(10 mins):

[0] Xtalks Announces Its Life Science Webinar Calendar for April 2019 [0] RoosterBio Will Attend Society for Biomaterials Annual Meeting April 3-6, 2019 [0] ASGCT To Honor Four Ground-Breaking Researchers at 22nd Annual Meeting

(Date:4/16/2019)... ... April 16, 2019 , ... ... technology, today announced that Bank South Pacific (BSP), the largest regionally owned bank ... bank awarded Fulcrum a contract to deliver a fingerprint-based Know Your Customer (KYC) ...

(Date:4/10/2019)... ... April 10, 2019 , ... After more than three months ... new kitchen is equipped with updated appliances and modern lighting and fixtures, and will ... , “We’ve seen tremendous growth in the demand for our team-building events ...

(Date:4/8/2019)... , ... April 08, 2019 , ... ... communications and collaboration solutions, announced today the launch of their app integration solution, ... range of business and contact-orientated applications while using CT Cloud Voice and Skype ...

(Date:4/4/2019)... ... April 03, 2019 , ... LeadingBiotech, ... collaboration and dialogue, today announces its Boston CEO conference to be held May ... industry’s challenges and opportunities with high-level panels and discussions from past, present and ...

Breaking Biology Technology:

[0] Bank South Pacific Selects Fulcrum Biometrics for Fingerprint-Based KYC Solution [0] Lajollacooks4u Set to Unveil Brand-New Kitchen [0] CallTower Empowers UCaaS Integration to an Unprecedented Range of Business Apps with CT Cloud Unite [0] LeadingBiotech’s Boston CEO Conference Unites Biopharma Leaders May 28-29 at Four Seasons Hotel