Navigation Links
Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Thymus from OriGene Technologies

ProductsRapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Thymus from OriGene Technologies
Company OriGene Technologies
Item Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Thymus
Price $925.00
Description The Rapid-Screen cDNA Library Panels were designed for full-length gene cloning and require just three sets of PCRs to identify the desired cDNA clone. The first PCR analysis is performed in a 96-well "Master Plate," where each well contains plasmid DNA from 5,000 clones. Having identified the positive well(s) by gel electrophoresis of the reaction products, the second PCR analysis is carried out in the corresponding 96-well "Sub-Plate(s)," which contains all 5,000 clones from the positive well of the Master Plate arrayed at only 50 clones per well. Since this plate contains glycerol stocks of E. coli instead of plasmid DNA, the cells can be diluted and plated out once a positive well(s) has been identified. Analysis of 96 single colonies by PCR leads to the identification of the positive clone from the Sub-Plate well. To ensure that a clone detected in a specific well in the Master Plate will be represented in the appropriate Sub-Plate, the Sub- Plates were not derived by dilution of individual wells from the Master Plate (a process which may lose specific clones). Instead, the Sub-Plates were generated first and the wells from each Sub-Plate were then pooled to generate the Master Plate. To guarantee that clones from different wells in the Master Plate were each independently-derived, the Sub-Plates were generated with primary transformants that had not been amplified.
Info OriGene TechnologiesOriGene Technologies
6 Taft Court, Suite 100
Rockville, MD 20850
USA

Call OriGene Technologies to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-866-592-4179
Customer Service: 1-888-267-4436 (U.S. only) / 301-340-3188
Fax Number: 301-340-9254
Web Site: http://www.origene.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Placenta from OriGene Technologies
2. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Heart from OriGene Technologies
3. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Testis from OriGene Technologies
4. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Brain (adult) from OriGene Technologies
5. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (12.5 - day) from OriGene Technologies
6. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (19 - day) from OriGene Technologies
7. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Rat Brain (adult) from OriGene Technologies
8. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Brain (fetal) from OriGene Technologies
9. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Lung from OriGene Technologies
10. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Colon from OriGene Technologies
11. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Spleen from OriGene Technologies
... 20-HETE is a cytochrome P450 (CYP450) ... in the renal and cerebral vasculature. In ... that mediates pressure-induced autoregulatory vasoconstriction. 20-HETE is ... concentration of free 20-HETE (20-40 pg/ml in ...
... The GPS-M Mutagenesis System enables you to ... design into,your target DNA by in vitro ... a Tn7-based,transposon randomly into the DNA target. ... BAC or purified,chromosomal DNA, depending on your ...
... c-Jun N-terminal kinase (JNK) is one ... Firefly luciferase has been used as a ... function. Luciferase provides a 1000-fold increase in ... acetyltransferase (CAT) assay. Firefly luciferase catalyzes the ...
... The FailSafe™ PCR System is the only ... first time and every time. It provides ... of the source or the property of ... FailSafe™ PCR Enzyme Mix, a unique blend ...
Biology Products:
(Date:12/4/2019)... ... 04, 2019 , ... NDA Partners Chairman Carl Peck, ... expert with more than 20 years of experience working in the biopharmaceutical industry, ... extensive experience with a variety of biologic modalities including monoclonal antibodies, bi-specific antibodies, ...
(Date:11/27/2019)... (PRWEB) , ... November 26, 2019 , ... Personalized ... event put on by the Arthritis Foundation and hosted by Dr. Steven Sampson and ... event, which took place on October 23, 2019, highlighted the ongoing efforts to promote ...
(Date:11/19/2019)... HERCULES, Calif. (PRWEB) , ... November 19, 2019 ... ... for epigenetics research and sample preparation, and Bio-Rad Laboratories, Inc. (NYSE: BIO and ... announced a partnership in which Diagenode will offer Single-Cell ATAC-Seq (scATAC-Seq) Services, featuring ...
Breaking Biology News(10 mins):
(Date:12/4/2019)... Kingdom (PRWEB) , ... December 03, 2019 , ... ... recombinant antibody products and services, today announced a partnership with the Recombinant Antibody ... University of Toronto, and UC San Francisco (UCSF) with a common goal to ...
(Date:12/4/2019)... ... December 04, 2019 , ... Improved Pharma’s analytical methods provide ... Additionally, their PDF Synchrotron methods provide a way to determine the structure of ... regulatory and patent applications.  , The latest developments were recently shared at the ...
(Date:11/27/2019)... ... November 25, 2019 , ... Silony Medical, a ... robotic-assisted and navigated surgery in the UK. The procedure was performed in the ... Dr. Syed Aftab and Dr. Alexander Montgomery. The surgery, which took place on ...
(Date:11/20/2019)... NEWTOWN, Conn. (PRWEB) , ... November 20, 2019 ... ... is projecting worldwide production of about 8,500 microturbine electrical power generation units from ... is a significant increase over 2017's projection of 3,821 units worth $569 million. ...
Breaking Biology Technology: