Navigation Links
Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Testis from OriGene Technologies

ProductsRapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Testis from OriGene Technologies
Company OriGene Technologies
Item Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Testis
Price $925.00
Description The Rapid-Screen cDNA Library Panels were designed for full-length gene cloning and require just three sets of PCRs to identify the desired cDNA clone. The first PCR analysis is performed in a 96-well "Master Plate," where each well contains plasmid DNA from 5,000 clones. Having identified the positive well(s) by gel electrophoresis of the reaction products, the second PCR analysis is carried out in the corresponding 96-well "Sub-Plate(s)," which contains all 5,000 clones from the positive well of the Master Plate arrayed at only 50 clones per well. Since this plate contains glycerol stocks of E. coli instead of plasmid DNA, the cells can be diluted and plated out once a positive well(s) has been identified. Analysis of 96 single colonies by PCR leads to the identification of the positive clone from the Sub-Plate well. To ensure that a clone detected in a specific well in the Master Plate will be represented in the appropriate Sub-Plate, the Sub- Plates were not derived by dilution of individual wells from the Master Plate (a process which may lose specific clones). Instead, the Sub-Plates were generated first and the wells from each Sub-Plate were then pooled to generate the Master Plate. To guarantee that clones from different wells in the Master Plate were each independently-derived, the Sub-Plates were generated with primary transformants that had not been amplified.
Info OriGene TechnologiesOriGene Technologies
6 Taft Court, Suite 100
Rockville, MD 20850
USA

Call OriGene Technologies to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-866-592-4179
Customer Service: 1-888-267-4436 (U.S. only) / 301-340-3188
Fax Number: 301-340-9254
Web Site: http://www.origene.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Placenta from OriGene Technologies
2. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Heart from OriGene Technologies
3. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Brain (adult) from OriGene Technologies
4. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (12.5 - day) from OriGene Technologies
5. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (19 - day) from OriGene Technologies
6. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Thymus from OriGene Technologies
7. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Rat Brain (adult) from OriGene Technologies
8. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Brain (fetal) from OriGene Technologies
9. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Lung from OriGene Technologies
10. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Colon from OriGene Technologies
11. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Spleen from OriGene Technologies
... Bio-Beads S-X media are neutral, ... size exclusion chromatography of lipophilic ... require organic eluents. The beads ... xylene, carbon tetrachloride, dimethylformamide, ketones, ...
TGase3 (S-20)...
15(S)-HEDE is produced from 11Z,14Z-eicosadienoic acid by 15-LO. 15(S)-HEDE is an inhibitor of RBL-1 cell 5-LO with an IC50 value of 26 µM....
Mouse monoclonal [HYB 232-02] to Protein S ( Abpromise for all tested applications). entrezGeneID: 5627 SwissProtID: P07225...
Biology Products:
(Date:2/13/2020)... (PRWEB) , ... February 13, 2020 , ... Murrieta ... startups, will be hosting a lunch and learn event on February 25th from 11:30 ... Entrepreneurs, researchers and interested members of the public are invited to attend this free ...
(Date:2/13/2020)... ... 13, 2020 , ... Worldwide Business with kathy ireland® ... Yoram Ashery to discuss how their revolutionary technology can kill bacteria and help ... people healthy with their revolutionary nanoparticle technology. Nobio is on the cutting edge ...
(Date:2/11/2020)... BALTIMORE (PRWEB) , ... February 11, 2020 , ... ... has moved into The LaunchPort™ medical device accelerator in Port Covington, Baltimore. The ... the issues that must be addressed to manage increased demand for home dialysis ...
Breaking Biology News(10 mins):
(Date:2/3/2020)... BUDAPEST, Hungary and CAMBRIDGE, Mass. (PRWEB) , ... ... ... headquartered in Budapest with US offices in Cambridge, MA, announce that OmniType, the ... Access Program (EAP) from today. OmniType is a best-in-class product featuring locus-multiplexing and ...
(Date:1/27/2020)... ... 2020 , ... Cerebyte, the leader in “instant mentoring” solutions ... the availability of neuroscience of transformational leadership workshops. These highly interactive, hands-on workshops ... and change research to become great transformational leaders. The workshops are ...
(Date:1/24/2020)... ... January 24, 2020 , ... ... RedySmart™ mass flow meters and controllers ideal for BioPharm OEMS. ... upgrade to antiquated rotameters. These thermal instruments employ high-precision MEMS (Micro-Electro Mechanical Systems) ...
(Date:1/22/2020)... ... January 21, 2020 , ... COBO Technologies and Cardea ... The partners have agreed to co-develop and market a portfolio of CRISPR QC ... programs. Over the longer-term, the parties aim to develop a COBO branded QC ...
Breaking Biology Technology: