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Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Kidney from OriGene Technologies

ProductsRapid-Screen Arrayed cDNA Library Panels Master Plates -Human Kidney from OriGene Technologies
Company OriGene Technologies
Item Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Kidney
Price $925.00
Description The Rapid-Screen cDNA Library Panels were designed for full-length gene cloning and require just three sets of PCRs to identify the desired cDNA clone. The first PCR analysis is performed in a 96-well "Master Plate," where each well contains plasmid DNA from 5,000 clones. Having identified the positive well(s) by gel electrophoresis of the reaction products, the second PCR analysis is carried out in the corresponding 96-well "Sub-Plate(s)," which contains all 5,000 clones from the positive well of the Master Plate arrayed at only 50 clones per well. Since this plate contains glycerol stocks of E. coli instead of plasmid DNA, the cells can be diluted and plated out once a positive well(s) has been identified. Analysis of 96 single colonies by PCR leads to the identification of the positive clone from the Sub-Plate well. To ensure that a clone detected in a specific well in the Master Plate will be represented in the appropriate Sub-Plate, the Sub- Plates were not derived by dilution of individual wells from the Master Plate (a process which may lose specific clones). Instead, the Sub-Plates were generated first and the wells from each Sub-Plate were then pooled to generate the Master Plate. To guarantee that clones from different wells in the Master Plate were each independently-derived, the Sub-Plates were generated with primary transformants that had not been amplified.
Info OriGene TechnologiesOriGene Technologies
6 Taft Court, Suite 100
Rockville, MD 20850
USA

Call OriGene Technologies to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-866-592-4179
Customer Service: 1-888-267-4436 (U.S. only) / 301-340-3188
Fax Number: 301-340-9254
Web Site: http://www.origene.com
NOTE: Price information is approximate list price and actual prices may vary.

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... fluorescent nucleic acid dye which binds only to ...erstain, propidium iodide stains the nucleus light...provides excellent contrast to green or blue fluor...ide can be used for fluorescent in situ hybridizat...
...ter assay for mitochondrial membrane potential det...are detected by carboxyfluorescein (FAM)-labelled ... are cell-permeable, non-cytotoxic and bind covale...he same time the mitochondrial membrane potential ...
...tured in DMEM and harvested at the log phase of gr...ve forms, cells were fixed in acetone-methanol. T...coated slide, with each wells surface specifically...imize background staining. Each well is loaded wi...
...r can be induced prior to DNA purification increas...y of single-copy BACs with the high yields associa...s provide the linearized vector and associated rea...stem) necessary to create a 10X human BAC library....
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