Navigation Links
Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Colon from OriGene Technologies

ProductsRapid-Screen Arrayed cDNA Library Panels Master Plates -Human Colon from OriGene Technologies
Company OriGene Technologies
Item Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Colon
Price $925.00
Description The Rapid-Screen cDNA Library Panels were designed for full-length gene cloning and require just three sets of PCRs to identify the desired cDNA clone. The first PCR analysis is performed in a 96-well "Master Plate," where each well contains plasmid DNA from 5,000 clones. Having identified the positive well(s) by gel electrophoresis of the reaction products, the second PCR analysis is carried out in the corresponding 96-well "Sub-Plate(s)," which contains all 5,000 clones from the positive well of the Master Plate arrayed at only 50 clones per well. Since this plate contains glycerol stocks of E. coli instead of plasmid DNA, the cells can be diluted and plated out once a positive well(s) has been identified. Analysis of 96 single colonies by PCR leads to the identification of the positive clone from the Sub-Plate well. To ensure that a clone detected in a specific well in the Master Plate will be represented in the appropriate Sub-Plate, the Sub- Plates were not derived by dilution of individual wells from the Master Plate (a process which may lose specific clones). Instead, the Sub-Plates were generated first and the wells from each Sub-Plate were then pooled to generate the Master Plate. To guarantee that clones from different wells in the Master Plate were each independently-derived, the Sub-Plates were generated with primary transformants that had not been amplified.
Info OriGene TechnologiesOriGene Technologies
6 Taft Court, Suite 100
Rockville, MD 20850
USA

Call OriGene Technologies to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-866-592-4179
Customer Service: 1-888-267-4436 (U.S. only) / 301-340-3188
Fax Number: 301-340-9254
Web Site: http://www.origene.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Placenta from OriGene Technologies
2. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Heart from OriGene Technologies
3. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Testis from OriGene Technologies
4. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Brain (adult) from OriGene Technologies
5. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (12.5 - day) from OriGene Technologies
6. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (19 - day) from OriGene Technologies
7. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Thymus from OriGene Technologies
8. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Rat Brain (adult) from OriGene Technologies
9. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Brain (fetal) from OriGene Technologies
10. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Lung from OriGene Technologies
11. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Spleen from OriGene Technologies
... multi-functional program that searches for ... sequence file for polymerase chain ... mutagenesis, and various hybridization applications. ... secondary structure of oligonucleotides based ...
... sequence alignment, 3D structure visualization, phylogenetic tree ... in a single application. ... Uniprot, sort and filter them in real-time ... it, rapidly searching, filtering and sorting ...
... Epitope Design consultation. Peptide synthesis and ... acids). Conjugation to KLH. 5mg ... of 2 New Zealand white female rabbits. ... and 4 production bleeds. Elisa testing ...
Transfection Buffer A & B Set 5 ml each...
Biology Products:
(Date:1/30/2019)... ... January 30, 2019 , ... ... supports high-growth, high-impact life science and biotechnology companies, announced today that it ... foundations and disease-focused venture funds to discuss their investment philosophies, explain their ...
(Date:1/24/2019)... ... January 23, 2019 , ... rHEALTH LLC, an ... of Allowance from the United States Patent and Trademark Office (USPTO) for U.S. ... Medicine Institute (DMI). This application covers the addition of a length scale to ...
(Date:1/20/2019)... ... January 16, 2019 , ... January has been a record-breaking month ... Not only has the company already hosted several of its signature cooking classes and ... and Janssen Pharmaceuticals, later this month. , Since 2008, Lajollacooks4u’s team-building events have ...
Breaking Biology News(10 mins):
(Date:2/12/2019)... ... February 12, 2019 , ... ... has finalized a deal to extend their use of Medrio, the leading provider ... 2023. The CRO has been a regular Medrio customer for over nine years, ...
(Date:2/2/2019)... ... January 31, 2019 , ... Dust off your Doc ... it hosts the Nuriv 2019 Expo West Music and Tech Festival at the ESports ... foster innovation in the natural foods, beverages, and dietary supplement industries, the Nuriv Music ...
(Date:2/2/2019)... ... February 01, 2019 , ... Stay on top of current hot ... food industries. Access to all webinars is free, so be sure to register today ... Visit http://www.xtalks.com to check out our upcoming webinars, or click below to ...
(Date:1/30/2019)... ... January 29, 2019 , ... ... today announced the dosing of the first patient in a phase 1/2 clinical ... serious disease associated with high morbidity and often leads to premature death. ...
Breaking Biology Technology: