Navigation Links
Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Brain (fetal) from OriGene Technologies

ProductsRapid-Screen Arrayed cDNA Library Panels Master Plates -Human Brain (fetal) from OriGene Technologies
Company OriGene Technologies
Item Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Brain (fetal)
Price $925.00
Description The Rapid-Screen cDNA Library Panels were designed for full-length gene cloning and require just three sets of PCRs to identify the desired cDNA clone. The first PCR analysis is performed in a 96-well "Master Plate," where each well contains plasmid DNA from 5,000 clones. Having identified the positive well(s) by gel electrophoresis of the reaction products, the second PCR analysis is carried out in the corresponding 96-well "Sub-Plate(s)," which contains all 5,000 clones from the positive well of the Master Plate arrayed at only 50 clones per well. Since this plate contains glycerol stocks of E. coli instead of plasmid DNA, the cells can be diluted and plated out once a positive well(s) has been identified. Analysis of 96 single colonies by PCR leads to the identification of the positive clone from the Sub-Plate well. To ensure that a clone detected in a specific well in the Master Plate will be represented in the appropriate Sub-Plate, the Sub- Plates were not derived by dilution of individual wells from the Master Plate (a process which may lose specific clones). Instead, the Sub-Plates were generated first and the wells from each Sub-Plate were then pooled to generate the Master Plate. To guarantee that clones from different wells in the Master Plate were each independently-derived, the Sub-Plates were generated with primary transformants that had not been amplified.
Info OriGene TechnologiesOriGene Technologies
6 Taft Court, Suite 100
Rockville, MD 20850
USA

Call OriGene Technologies to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-866-592-4179
Customer Service: 1-888-267-4436 (U.S. only) / 301-340-3188
Fax Number: 301-340-9254
Web Site: http://www.origene.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Placenta from OriGene Technologies
2. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Heart from OriGene Technologies
3. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Testis from OriGene Technologies
4. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Brain (adult) from OriGene Technologies
5. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (12.5 - day) from OriGene Technologies
6. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (19 - day) from OriGene Technologies
7. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Thymus from OriGene Technologies
8. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Rat Brain (adult) from OriGene Technologies
9. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Lung from OriGene Technologies
10. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Colon from OriGene Technologies
11. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Spleen from OriGene Technologies
Anti-CD74 Monoclonal Antibody Description: 200 l Research Focus: cell surface antigens Storage: 4C Shipping Temperature: 4C...
POU domain, class 2, transcription factor 2,...
HSPBP1 Antibody...
golgi reassembly stacking protein 2, 55kDa...
Biology Products:
(Date:7/9/2014)... era of increasing concern about the prevalence of ... a promising new pathway to disabling disease: blocking ... The scientists showed how bacterial siderophore, a small ... to fan bacterial growth as well as ... this infection process. Their findings appear in a ...
(Date:7/9/2014)... first-ever global census of Adlie penguins shows that ... 53 percent larger than previously estimated. Adlie penguins ... to monitor and understand the effects of climate ... By using high-resolution satellite imagery, researchers from Stony ... a new method that permits regular monitoring of ...
(Date:7/9/2014)... accrue. To completely and systematically archive this body ... researchers in the life sciences spend a quarter ... online survey of 70 people working in biology ... Information Technology FIT in Sankt Augustin. Many of ... or structured approach to data collection in their ...
Breaking Biology News(10 mins):Bacteria hijack plentiful iron supply source to flourish 2New study finds that Adélie penguin population is on the rise 2New study finds that Adélie penguin population is on the rise 3Managing the data jungle 2Managing the data jungle 3
... Traffic accidents involving wildlife are on the rise in Europe. ... be useful for increasing safety and preventing human and animal ... researchers have established at what time, on what day of ... roe deer are most likely to take place. Car ...
... that cancerous tumors grow collections of abnormal blood cells, the ... new evidence in an animal model suggests that blood vessels ... same purposeand could provide the key to a new way ... L. Makey, Edmund Chinchar, Carissa Howie, and Lucio Miele, all ...
... Animal sanctuaries can play an important role in rehabilitating goats ... scientists at Queen Mary, University of London. In ... moods in 18 goats, nine of which had endured poor ... shelter before arriving at a sanctuary. They created a spatial ...
Cached Biology News:Roe deer more likely to be run over at nightfall on a Sunday in April 2Drug reduces fat by blocking blood vessels 2Drug reduces fat by blocking blood vessels 3Rescue me: New study finds animals do recover from neglect 2
(Date:1/15/2014)... 15, 2014  Bill Jacobs Automotive, a group of car dealerships ... with the Heartland Blood Center and offering free oil change ... takes place Saturday, Jan. 18 at the Bill Jacobs Cadillac ... (Photo: http://photos.prnewswire.com/prnh/20140115/MN46637 )  The Bill ...
(Date:1/15/2014)... California , January 15, 2014 Oxford ... today announced the appointment of Thomas C Reynolds MD, PhD ... years, development experience gained in the biotechnology industry, most recently ... am delighted to welcome Tom at this transformative time for ...
(Date:1/15/2014)... Two champions of science, technology, engineering, ... an annual competition for middle and high school students ... STEM study. The competition presents students with real-world problems ... Aptitude, Mathematics, and Sciences is a program administered ...
(Date:1/15/2014)... (PRWEB) January 15, 2014 AudioNotch is ... new therapy for the treatment of tinnitus. Patients listen ... frequency, and over a period of weeks to months, their ... Therapy in two forms: Notched Music and Notched White Noise. ...
Breaking Biology Technology:Join the Bill Jacobs Auto blood drive and receive a free oil change 2Oxford BioTherapeutics Appoints Thomas C Reynolds MD, PhD to its Board of Directors 2Oxford BioTherapeutics Appoints Thomas C Reynolds MD, PhD to its Board of Directors 3Technology Student Association Partners with Leaders in STEM Education Advocacy for Annual Competition 2Technology Student Association Partners with Leaders in STEM Education Advocacy for Annual Competition 3Technology Student Association Partners with Leaders in STEM Education Advocacy for Annual Competition 4Technology Student Association Partners with Leaders in STEM Education Advocacy for Annual Competition 5