Rabbit Anti-SKAP55 Polyclonal Antibody, Unconjugated from ABR-Affinity BioReagents

PA1-525 detects human SKAP55. PA1-525 has been successfully used in Western blot, immunocytochemistry and immunoprecipitation procedures. By Western blot, this antibody detects an ~ 60 kDa protein from human Jurkat cells.This antibody has also been used in immunoprecipitation and immunocytochemical procedures. PA1-525 antigen corresponds to amino acids 2-105 of human SKAP-55.Src kinase-associated phosphoprotein of 55 kDa (SKAP55) is expressed as a constitutively tyrosine phosphorylated 55-kD adapter protein that positively regulates TCR signaling. SKAP55 has also been shown to enhance adhesion to fibronectin and ICAM1, colocalize with F-actin at the T cell-antigen-presenting cell synapse and promotes the clustering of LFA1 and TCR ligation. TCR engagement induces the translocation of SKAP55 to lipid rafts, where the interactions with Fyn take place. CD45 also plays a very important role in SKAP55/TCR-mediated signaling. SKAP55 involvement in the TCR signaling pathway is the subject of current research in murine as well as human models.