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Rabbit Anti-Mint3 Polyclonal Antibody, Unconjugated from ABR-Affinity BioReagents

ProductsRabbit Anti-Mint3 Polyclonal Antibody, Unconjugated from ABR-Affinity BioReagents
Company ABR-Affinity BioReagents
Item Rabbit Anti-Mint3 Polyclonal Antibody, Unconjugated
Price $295.00
Description Immunogen: Synthetic Peptide: M(1) E F L P E P Q H P P G P P T M D L E(19)

PA1-072 detects munc-18 interacting protein 3 (Mint3) from mouse cells. PA1-072 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~86 kDa protein representing Mint3 from AtT20 cell extract. PA1-072 immunizing peptide corresponds to amino acid residues 1-19 from rat Mint3 protein. PA1-072 immunizing peptide (Cat. # PEP-135) is available for use in neutralization and control experiments.The munc-18 interacting protein (Mint) protein family is a group of evolutionarily conserved adaptor proteins that function in membrane transport and organization. In mammals, there exist three mint isoforms, Mint1, 2, and 3. Although there is little amino acid sequence conservation in the amino-terminal half, the carboxy-terminal half of these proteins is highly conserved. Within this conserved portion there exists a phosphotyrosine-binding (PTB) and a PSD-95/DLG-A/ZO-1 (PDZ) domain, which function as protein interaction modules. Mint1 and 2 appear to be expressed exclusively in the brain and are found to bind to Munc18, an essential component of the synaptic vesicle fusion machinery. Mint3 is ubiquitously expressed in all tissues and is expressed at the lowest levels in the brain and testis. Studies show that mint3 does not interact with munc-18. Mint3 has been found to interact with the Alzheimers Disease-related amyloid precursor protein (APP) and does so through its PTB and PDZ domains. It has been suggested that mint3 links APP to other transport machinery components, thereby regulating it transport, endocytosis, and metabolism. Abnormal APP metabolism has been shown to be the cause of an early-onset type of Alzheimers disease.

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