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RTS AviTag E. coli Biotinylation Kit, Linear Template; Linear template generation and in vitro protein mono-biotinylation system for use with RTS 100 expression reactions from Roche Applied Science

ProductsRTS AviTag E. coli Biotinylation Kit, Linear Template; Linear template generation and in vitro protein mono-biotinylation system for use with RTS 100 expression reactions from Roche Applied Science
Company Roche Applied Science
Item RTS AviTag E. coli Biotinylation Kit, Linear Template; Linear template generation and in vitro protein mono-biotinylation system for use with RTS 100 expression reactions
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Description Roches RTS AviTag E. coli Biotinylation Kit, Linear Template is designed for use with high-yield RTS 100 E. coli expression kits. (Cat. No. 3 186 148, 3 186 156). The kit provides reagents to rapidly generate linear expression constructs by PCR containing and optimized control region for expression based on a combination of T7 RNA polymerase and prokaryotic cell lysate. Flexible positioning of AviTag [peptide sequence GLNDIFEAQKIEWHE] is possible. The linear constructs are ready for use as DNA templates in RTS 100 E. coli HY in vitro protein expression reactions without the need for purification.The expressed protein can be enzymatically mono-biotinylated by E. coli biotin protein ligase (BirA, E.C. 6.3.4.15). In addition to E. coli Bir A the kit supplies a substrate energy mixture, which is required for the biotinylation reaction.
Info Roche Applied ScienceRoche Applied Science
Roche Diagnostics Corporation
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9115 Hague Road
Indianapolis, IN 46250-0414 USA




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