Navigation Links
Pyrophosphate Fluorometric (Blue) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)

ProductsPyrophosphate Fluorometric (Blue) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
Company Molecular Probes (Invitrogen)
Item Pyrophosphate Fluorometric (Blue) Assay Kit, EnzChek®
Price 
Description Molecular Probes EnzChek Pyrophosphate Detection Kit provides a fast, convenient and inexpensive spectrophotometric method for measuring the inorganic pyrophosphate (PPi ) produced by a number of biochemical reactions, such as DNA and RNA polymerizations, cyclic AMP formation by the enzyme adenylate cyclase and the enzymatic activation of fatty acids to form their coenzyme A esters.1 The EnzChek Pyrophosphate Assay Kit is a modification of our EnzChek Phosphate Assay Kit (E-6646), which is based on the method originally described by Webb.2 In the presence of inorganic phosphate (Pi ), the substrate 2-amino-6-mercapto-7-methylpurine ribonucleoside (MESG) is converted enzymatically by purine nucleoside phosphorylase (PNP) to ribose 1-phosphate and 2-amino-6-mercapto-7-methylpurine (Figure 1). Enzymatic conversion of MESG results in a shift in absorbance maximum from 330 nm for the substrate to 360 nm for the product.

The EnzChek Pyrophosphate Assay Kit includes the enzyme inorganic pyrophosphatase, which catalyzes conversion of PPi present into two equivalents of Pi. The Pi is then consumed by the MESG/PNP reaction and detected by an increase in absorbance at 360 nm. Additional sensitivity is gained by the amplification of one molecule of PPi to two molecules of Pi. The assay can detect as little as 1 nanomole of PPi in 1 mL reactions (Figure 2) and can be performed over a pH range of 6.5 to 8.5.2 The MESG/PNP reaction has been used for continuous spectrophotometric determination of aminoacyl-tRNA synthetase activity.3 In addition, we have tested the ability of this assay to detect the PPi liberated by the activities of acetyl-CoA synthetase (Figure 3) and luciferase (Figure 4). In these experiments, we have detected as few as 5 10-5 U of acetyl-CoA synthetase activity and 10-2 U of luciferase activity.

Info Molecular Probes (Invitrogen)Molecular Probes (Invitrogen)
PO Box 22010
Eugene, OR 97402-0469

Call Molecular Probes (Invitrogen) to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-541-359-2658
Customer Service: 1-541-465-8300
Fax Number: 1-541-344-6504
Web Site: http://probes.invitrogen.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Pyrophosphate Fluorometric (Blue) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
2. Pyrophosphate Fluorometric (Blue) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
3. Pyrophosphate Colorimetric / Fluorometric (resorufin) Assay Kit, PiPer from Molecular Probes (Invitrogen)
4. Hydrogen Peroxide / Peroxidase Fluorometric (resorufin) Assay Kit, Fluoro H2O2 from BACHEM
5. 20S Proteasome Fluorometric (AMC) Assay Kit from CHEMICON
6. Cell invasion Fluorometric (green) Assay Kit, InnoCyte™ from Calbiochem
7. Telomerase Fluorometric Assay Kit, TRAPeze from CHEMICON
8. TACE Fluorometric (MCA)) Assay Kit, InnoZyme™ from Calbiochem
9. Lysozyme Fluorometric (Green) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
10. Amylase Fluorometric (Green) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
11. Amylase Fluorometric (Green) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
(Date:4/5/2017)... Allen Institute for Cell Science today announces the launch ... dynamic digital window into the human cell. The website ... deep learning to create predictive models of cell organization, ... suite of powerful tools. The Allen Cell Explorer will ... resources created and shared by the Allen Institute for ...
(Date:4/4/2017)... 4, 2017   EyeLock LLC , a leader ... United States Patent and Trademark Office (USPTO) has issued ... linking of an iris image with a face image ... the company,s 45 th issued patent. ... timely given the multi-modal biometric capabilities that have recently ...
(Date:3/30/2017)... 2017 The research team of The Hong ... fingerprint identification by adopting ground breaking 3D fingerprint minutiae recovery and ... speed and accuracy for use in identification, crime investigation, immigration control, ... ... A research team led ...
Breaking Biology News(10 mins):
(Date:10/12/2017)... , ... October 12, 2017 ... ... announces publication of a United States multicenter, prospective clinical study that demonstrates ... point-of-care diagnostic test capable of identifying clinically significant acute bacterial and viral ...
(Date:10/11/2017)... ... 2017 , ... At its national board meeting in North ... the co-founder, CEO and chief research scientist of Minnesota-based Advanced Space Technology and ... ARCS Alumni Hall of Fame . ASTER Labs is a technology development ...
(Date:10/11/2017)... Md. (PRWEB) , ... October 11, 2017 , ... ... digital pathology, announced today it will be hosting a Webinar titled, “Pathology is ... Advanced Pathology Associates , on digital pathology adoption best practices and how Proscia ...
(Date:10/11/2017)... ... October 11, 2017 , ... A new study published in ... and fresh in vitro fertilization (IVF) transfer cycles. The multi-center matched ... , After comparing the results from the fresh and frozen transfer cohorts, the ...
Breaking Biology Technology: