Navigation Links
ProtoArray Yeast PPI Kit from Invitrogen

ProductsProtoArray Yeast PPI Kit from Invitrogen
Company Invitrogen
Item ProtoArray Yeast PPI Kit
Price 
Description The Yeast ProtoArray high-density functional protein microarray is the first microarray product that enables rapid elucidation of proteinprotein interactions on a proteome scale. The Yeast ProtoArray contains 4,088 S. cerevisiae open reading frames (ORFs) expressed as 5'-GST fusions, purified and spotted in duplicate on a 1 inch x 3 inch nitrocellulose-coated slide. With the Yeast ProtoArray PPI Kit, you can identify novel protein interactions in a single day, instead of the several weeks required using alternatives such as yeast two-hybrid systems. In addition, protein interactions can be easily assayed under different conditions to better understand interaction dynamics.


Using the Yeast ProtoArray PPI Kit, protein-protein interactions screening takes four simple steps:
Prepare your protein of interest using the included ProtoArray biotinylation reagents. • Probe the Yeast ProtoArray PPI microrray using the biotinylated protein of interest • Detect positive interactions using streptavidin-conjugated Alexa Fluor 647. • Dry and scan with most standard microarray readers.


Contents:
The complete kit contains two ProtoArray Yeast Proteome and two Control Microarrays, ProtoArray Buffer Module A, ProtoArray Buffer Module B, Mini-Biotinylation Module kit, Biotinylation Purification Module, Biotinylation Assessment Module, 30 l (2 mg/ml) streptavidinconjugate Alexa Fluor(r) 647, and 40 l (0.5 mg/ml) biotinylated calmodulin kinase. The ProtoArray Buffer Module A contains 30 ml of 30% BSA, 400 l 1M DTT. The ProtoArray Buffer Module B contains blocking and probing buffers, 4 Hybrislips coverslips, 2 hybridization chambers. The ProtoArray Mini-Biotinylation Module Kit contains 100 g of Biotin-XX, sulfosuccinimidyl ester, sodium salt, 50 g SA, and 20 ng biotinylated BSA. The ProtoArray Biotinylation Purification Module contains the purification resin in storage buffer, 4 spin columns, and 8 collection tubes. The ProtoArray Biotinylation Assessment Module contains 20 l Streptavidin AP, and 5 ml AP substrate and buffers.
Info InvitrogenInvitrogen
Invitrogen Corporation
1600 Faraday Ave.
Carlsbad, CA 92008

Call Invitrogen to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-866-521-4270
Customer Service: 800-955-6288
Tech Support: 800-955-6288 ext. 2
Fax Number: 760-603-7229
Web Site: http://www.invitrogen.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. ProtoArray Kinase Substrate Identification Application Kit from Invitrogen
2. Yeast Nitrogen Base from Invitrogen
3. Yeast Nitrogen Base from Invitrogen
4. pYD1 Yeast Display Vector from Invitrogen
5. Yeast Protein Kit from Zymo Research
6. Yeast-GFP 96-well Plates from Invitrogen
7. Yeast-GFP Collection from Invitrogen
8. Yeast Oligo Microarray Kit (V2), 2 x 11K from Agilent Technologies
9. Frozen-EZ Yeast Transformation II Kit from Zymo Research
10. Hybrid Hunter Yeast Two-Hybrid System from Invitrogen
11. Yeast Nitrogen Base from Invitrogen
Control primers and template for 5kb and 15 kb products....
0.2ml Gradient Block MBSR. Robot compatible block ejection mechanism. Stackable. (96 well block - holds 1 plate 0.2ml x 96 well plate)...
A robust formulation of Taq Polymerase supplied with a 10X colored reaction buffer which allows Direct Gel-Loading...
A formulation of our Biolase DNA Polymerase which contains a Red Loading Dye...
Biology Products:
(Date:12/7/2016)... 2016   Veridium , a leader in ... new CEO James Stickland . Stickland, a ... experience, has served in senior executive roles for ... in expanding a pipeline of venture capital and ... recently served as managing director of U.K.-based fintech ...
(Date:12/6/2016)... 2016 Securus Technologies, a leading provider ... public safety, investigation, corrections and monitoring, and the ... five (5) year funding commitment by Securus to ... rehabilitation and reentry support to more inmates and ... 2004, the Prison Entrepreneurship Program (PEP) is an ...
(Date:12/2/2016)... , Dec. 1, 2016   SoftServe , ... BioLock , an electrocardiogram (ECG) biosensor analysis ... a key IoT asset. The smart system ensures ... vehicle,s steering wheel and mobile devices to easily ... As vehicle technology advances, so too ...
Breaking Biology News(10 mins):
(Date:12/7/2016)... ... December 07, 2016 , ... Huffman Engineering, ... become a Wonderware Certified System Integrator Partner. Huffman Engineering is the only ... , “The System Integrator Partner certification gives customers confidence that our engineers are ...
(Date:12/7/2016)... /PRNewswire/ - OncoQuest Inc. ("OncoQuest"), a biopharmaceutical company ... products for the treatment of cancer, today announced ... Development Program with Cytovance Biologics ( Oklahoma ... antibody product. Supported by recent positive interim clinical ... ovarian cancer patients, OncoQuest has engaged Cytovance to ...
(Date:12/7/2016)... Ontario , Dec. 7, 2016  Nordion, ... with General Atomics (GA), welcome today,s award by ... Nuclear Security Administration (NNSA) of the Phase II ... project with Nordion and the University of Missouri ... II funding will support the establishment of a ...
(Date:12/7/2016)... , December 8, 2016 The report "Acrylic Processing Aid Market by ... Packaging, Automotive, Consumer Goods) - Global Forecast to 2026", published by MarketsandMarkets, the ... reach USD 1,173.6 Million by 2026, registering an of CAGR of 6.2% between ... ... MarketsandMarkets Logo ...
Breaking Biology Technology: