Navigation Links
ProtoArray Yeast PPI Kit from Invitrogen

ProductsProtoArray Yeast PPI Kit from Invitrogen
Company Invitrogen
Item ProtoArray Yeast PPI Kit
Description The Yeast ProtoArray high-density functional protein microarray is the first microarray product that enables rapid elucidation of proteinprotein interactions on a proteome scale. The Yeast ProtoArray contains 4,088 S. cerevisiae open reading frames (ORFs) expressed as 5'-GST fusions, purified and spotted in duplicate on a 1 inch x 3 inch nitrocellulose-coated slide. With the Yeast ProtoArray PPI Kit, you can identify novel protein interactions in a single day, instead of the several weeks required using alternatives such as yeast two-hybrid systems. In addition, protein interactions can be easily assayed under different conditions to better understand interaction dynamics.

Using the Yeast ProtoArray PPI Kit, protein-protein interactions screening takes four simple steps:
Prepare your protein of interest using the included ProtoArray biotinylation reagents. • Probe the Yeast ProtoArray PPI microrray using the biotinylated protein of interest • Detect positive interactions using streptavidin-conjugated Alexa Fluor 647. • Dry and scan with most standard microarray readers.

The complete kit contains two ProtoArray Yeast Proteome and two Control Microarrays, ProtoArray Buffer Module A, ProtoArray Buffer Module B, Mini-Biotinylation Module kit, Biotinylation Purification Module, Biotinylation Assessment Module, 30 l (2 mg/ml) streptavidinconjugate Alexa Fluor(r) 647, and 40 l (0.5 mg/ml) biotinylated calmodulin kinase. The ProtoArray Buffer Module A contains 30 ml of 30% BSA, 400 l 1M DTT. The ProtoArray Buffer Module B contains blocking and probing buffers, 4 Hybrislips coverslips, 2 hybridization chambers. The ProtoArray Mini-Biotinylation Module Kit contains 100 g of Biotin-XX, sulfosuccinimidyl ester, sodium salt, 50 g SA, and 20 ng biotinylated BSA. The ProtoArray Biotinylation Purification Module contains the purification resin in storage buffer, 4 spin columns, and 8 collection tubes. The ProtoArray Biotinylation Assessment Module contains 20 l Streptavidin AP, and 5 ml AP substrate and buffers.
Info InvitrogenInvitrogen
Invitrogen Corporation
1600 Faraday Ave.
Carlsbad, CA 92008

Call Invitrogen to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-866-521-4270
Customer Service: 800-955-6288
Tech Support: 800-955-6288 ext. 2
Fax Number: 760-603-7229
Web Site:
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. ProtoArray Kinase Substrate Identification Application Kit from Invitrogen
2. Yeast Nitrogen Base from Invitrogen
3. Yeast Nitrogen Base from Invitrogen
4. pYD1 Yeast Display Vector from Invitrogen
5. Yeast Protein Kit from Zymo Research
6. Yeast-GFP 96-well Plates from Invitrogen
7. Yeast-GFP Collection from Invitrogen
8. Yeast Oligo Microarray Kit (V2), 2 x 11K from Agilent Technologies
9. Frozen-EZ Yeast Transformation II Kit from Zymo Research
10. Hybrid Hunter Yeast Two-Hybrid System from Invitrogen
11. Yeast Nitrogen Base from Invitrogen
... ,This solution has effect on enhancing an ... signal than the one obtained by conventional ... secondary antibody used and exposure time in ... images with high S/N ratio and low ...
... enzyme exists predominantly as XDH which converts ... critical plasma antioxidant. During ischemia, XDH ... producing XO by oxidation of essential sulfhydryl ... of the amino-terminus. AO has a ...
... the gap between the 500 l and ... the speed of the classic Vivaspin products ... for superior recoveries from very dilute solutions. ... Cellulose Triacetate membranes, Vivaspin 2 offers the ...
Goat polyclonal to Lipocalin 2...
Biology Products:
(Date:6/22/2016)... June 22, 2016 On Monday, the Department ... industry to share solutions for the Biometric Exit Program. ... and Border Protection (CBP), explains that CBP intends to ... the United States , in order ... defeat imposters. Logo - ...
(Date:6/16/2016)... The global Biometric ... USD 1.83 billion by 2024, according to a ... proliferation and increasing demand in commercial buildings, consumer ... the market growth.      (Logo: ... of advanced multimodal techniques for biometric authentication and ...
(Date:6/9/2016)... TURKU, Finland , June 9, 2016 ... French National Police deploy Teleste,s video security solution to ensure ... France during the major tournament ... and data communications systems and services, announced today that its ... Police Prefecture to back up public safety across ...
Breaking Biology News(10 mins):
(Date:6/24/2016)... June 24, 2016  Regular discussions on a range of ... between the two entities said Poloz. Speaking at ... Ottawa , he pointed to the country,s inflation target, ... government. "In certain ... institutions have common economic goals, why not sit down and ...
(Date:6/24/2016)... ... 24, 2016 , ... While the majority of commercial spectrophotometers and fluorometers use ... 6000i models are higher end machines that use the more unconventional z-dimension of 20mm. ... from the bottom of the cuvette holder. , FireflySci has developed several Agilent ...
(Date:6/23/2016)... ... June 23, 2016 , ... Mosio, ... second eBook, “Clinical Trials Patient Recruitment and Retention Tips.” Partnering with experienced clinical ... eBook by providing practical tips, tools, and strategies for clinical researchers. , “The ...
(Date:6/23/2016)... 2016   Boston Biomedical , an industry ... to target cancer stemness pathways, announced that its ... Drug Designation from the U.S. Food and Drug ... including gastroesophageal junction (GEJ) cancer. Napabucasin is an ... cancer stemness pathways by targeting STAT3, and is ...
Breaking Biology Technology: