Navigation Links
ProFound Label Transfer Sulfo SBED Protein:Protein Interaction Reagent from Pierce Biotechnology, Inc.

ProductsProFound Label Transfer Sulfo SBED Protein:Protein Interaction Reagent from Pierce Biotechnology, Inc.
Company Pierce Biotechnology, Inc.
Item ProFound Label Transfer Sulfo SBED Protein:Protein Interaction Reagent
Price 
Description ProFound Label Transfer Sulfo SBED Protein:Protein Interaction Reagent

A powerful new method known as Label Transfer has rapidly gained traction as an in vitro method for protein interaction discovery. A growing number of publications feature the exclusive Pierce ProFound Sulfo-SBED Biotin Label Transfer Reagent to study a variety of issues relating to protein interactions. • The use of Sulfo-SBED has contributed greatly to published studies on protein:protein interactions. To simplify the method, Pierce has assembled the key components frequently cited in publications featuring Sulfo-SBED in the label transfer application. The resulting easy-to-use kit also contains Streptavidin-HRP for subsequent detection of the biotinylated complex or biotinylated prey protein on a Western blot. •

Label transfer is an in vitro method for discovering or verifying an interaction that was observed by way of another technique such as the in vivo yeast 2-hybrid method or by in vitro methods such as co-immunoprecipitation or pull-down assays. • The label transfer method requires a purified "bait" protein. The amine groups of the bait protein are modified via the Sulfo-NHS moiety of Sulfo-SBED. This derivatized bait protein is then incubated in the dark with a lysate or with a purified putative "prey" protein. The bait and prey complex, if formed, is then captured or trapped by exposing the sample to high-intensity UV light, which activates the phenylazide moiety of Sulfo-SBED. This photoreactive group covalently links to the bound prey protein, capturing the interacting complex. Upon reduction of this complex, the biotin label that first resided with the bait protein transfers to the prey protein. The biotin label also functions as the detection target for the bait:prey complex or the prey protein upon Western blot analysis. The resulting biotin derivatized complex or prey protein can be detected using streptavidin-HRP or an anti-biotin antibody and chemiluminescent detection. The biotin label can also be used as a handle to purify the bait:prey complex over an avidin/streptavidin/NeutrAvidin/monomeric avidin biotin-binding protein support.

  • Amine group-specific reactivity
  • Nonspecific photoreactivity
  • Thiol-cleavable disulfide (S-S) linkage
  • A Biotin handle: This biotin transfers from a labeled purified bait protein to a captured prey protein (Figure 2), which makes it especially useful for Western blot detection
Info Pierce Biotechnology, Inc.Pierce Biotechnology, Inc.
Pierce Biotechnology, Inc.
3747 N. Meridian Rd.
P.O. Box 117
Rockford, IL 61105
Customer Service: 815-968-0747
Fax Number: 815-968-8148
Web Site: http://www.piercenet.com/
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Label IT Tracker CX-Rhodamine Intracellular Nucleic Acid Localization Kit from Mirus Bio Corporation
2. Label IT Tracker Cy5 Intracellular Nucleic Acid Localization Kit from Mirus Bio Corporation
3. Label IT Tracker BiotinIntracellular Nucleic Acid Localization Kit from Mirus Bio Corporation
4. Label IT siRNA Tracker Fluorescein Kit with TransIT-siQUEST Reagent from Mirus Bio Corporation
5. Label IT siRNA Tracker CX-Rhodamine Intracellular Localization Kit with TransIT-TKO siRNA Transfection Reagent from Mirus Bio Corporation
6. Label IT siRNA Tracker CX Rhodamine Kit with TransIT-siQUEST Reagent from Mirus Bio Corporation
7. Label IT siRNA Tracker Intracellular Localization Kit with TransIT-TKO Transfection Reagent (Cy5 Labeling Kit) from Mirus Bio Corporation
8. Label IT siRNA Tracker Fluorescein Intracellular Localization Kit with TransIT-TKO siRNA Transfection Reagent from Mirus Bio Corporation
9. Label IT siRNA Tracker TM Rhodamine Kit with TransIT-siQUEST Reagent from Mirus Bio Corporation
10. Label IT siRNA Tracker Biotin Kit with TransIT-siQUEST Reagent from Mirus Bio Corporation
11. Label IT siRNA Tracker Cy3 Kit with TransIT-siQUEST Reagent from Mirus Bio Corporation
... designed for RNA aptamer screening and binding ... powerful Paraflo microfluidic on-chip synthesis platform. These ... comprehensive DNA/RNA Aptamer Microarray Service. ... Aptamer Microarray contains greater than 1500 known ...
... Sciences provides a comprehensive service for ... density peptide microarrays synthesized on Paraflo ... recombinant proteins/antibodies or serum samples. This ... sequence designs, binding assays using sample(s) ...
... aptamer screening and binding optimization and built ... on-chip synthesis platform. Thousands of customer specified ... LC Sciences can provide assistance with custom ... part of our comprehensive DNA/RNA Aptamer Microarray ...
... (GST) is commonly used as a fusion ... (1). The GSTTag sequence has been reported ... cases the solubility of its fusion partners. ... form, GSTTag fusion proteins can be purified ...
Biology Products:
(Date:6/22/2016)... LOS ANGELES , June 22, 2016 ... of identity management and verification solutions, has ... cutting edge software solutions for Visitor Management, ... ® provides products that add functional ... The partnership provides corporations and venues with ...
(Date:6/21/2016)... June 21, 2016 NuData Security announced today ... role of principal product architect and that ... of customer development. Both will report directly to ... The moves reflect NuData,s strategic growth in its ... high customer demand and customer focus values. ...
(Date:6/16/2016)... The global Biometric ... USD 1.83 billion by 2024, according to a ... proliferation and increasing demand in commercial buildings, consumer ... the market growth.      (Logo: ... of advanced multimodal techniques for biometric authentication and ...
Breaking Biology News(10 mins):
(Date:11/30/2016)... 2016 Part of 5m$ Investment in ... ... Aptuit, LLC today announced that it had successfully completed the ... compounds have increased the Screening Collection to over 400,000. The ... capabilities of the company. This expansion, complemented by new robotics ...
(Date:11/30/2016)... ... November 30, 2016 , ... ... advanced technology applications, introduces the 5th generation, ultra-bright, Laser-Driven Light Source, the EQ-77, ... Light Source (LDLS™) technology, the EQ-77 offers higher radiance and irradiance from a ...
(Date:11/30/2016)... ... November 30, 2016 , ... With growth rates averaging more than 30% ... and look forward to continuing their expansion in their new office space. The new ... been traditionally favoured by the creative industries, so Random42 Scientific Communication will fit right ...
(Date:11/30/2016)... GREENWICH, Connecticut , November 30, 2016 ... für die integrierte Medikamententdeckung    ... , , ... führende Prüfbibliothek erfolgreich erweitert zu haben. Zusätzliche 150.000 neuartige ... neuen Substanzen wurden sorgfältig ausgewählt, um die starken Entdeckungsfähigkeiten ...
Breaking Biology Technology: