Navigation Links
ProFound Label Transfer Sulfo SBED Protein:Protein Interaction Reagent from Pierce Biotechnology, Inc.

ProductsProFound Label Transfer Sulfo SBED Protein:Protein Interaction Reagent from Pierce Biotechnology, Inc.
Company Pierce Biotechnology, Inc.
Item ProFound Label Transfer Sulfo SBED Protein:Protein Interaction Reagent
Price 
Description ProFound Label Transfer Sulfo SBED Protein:Protein Interaction Reagent

A powerful new method known as Label Transfer has rapidly gained traction as an in vitro method for protein interaction discovery. A growing number of publications feature the exclusive Pierce ProFound Sulfo-SBED Biotin Label Transfer Reagent to study a variety of issues relating to protein interactions. • The use of Sulfo-SBED has contributed greatly to published studies on protein:protein interactions. To simplify the method, Pierce has assembled the key components frequently cited in publications featuring Sulfo-SBED in the label transfer application. The resulting easy-to-use kit also contains Streptavidin-HRP for subsequent detection of the biotinylated complex or biotinylated prey protein on a Western blot. •

Label transfer is an in vitro method for discovering or verifying an interaction that was observed by way of another technique such as the in vivo yeast 2-hybrid method or by in vitro methods such as co-immunoprecipitation or pull-down assays. • The label transfer method requires a purified "bait" protein. The amine groups of the bait protein are modified via the Sulfo-NHS moiety of Sulfo-SBED. This derivatized bait protein is then incubated in the dark with a lysate or with a purified putative "prey" protein. The bait and prey complex, if formed, is then captured or trapped by exposing the sample to high-intensity UV light, which activates the phenylazide moiety of Sulfo-SBED. This photoreactive group covalently links to the bound prey protein, capturing the interacting complex. Upon reduction of this complex, the biotin label that first resided with the bait protein transfers to the prey protein. The biotin label also functions as the detection target for the bait:prey complex or the prey protein upon Western blot analysis. The resulting biotin derivatized complex or prey protein can be detected using streptavidin-HRP or an anti-biotin antibody and chemiluminescent detection. The biotin label can also be used as a handle to purify the bait:prey complex over an avidin/streptavidin/NeutrAvidin/monomeric avidin biotin-binding protein support.

  • Amine group-specific reactivity
  • Nonspecific photoreactivity
  • Thiol-cleavable disulfide (S-S) linkage
  • A Biotin handle: This biotin transfers from a labeled purified bait protein to a captured prey protein (Figure 2), which makes it especially useful for Western blot detection
Info Pierce Biotechnology, Inc.Pierce Biotechnology, Inc.
Pierce Biotechnology, Inc.
3747 N. Meridian Rd.
P.O. Box 117
Rockford, IL 61105
Customer Service: 815-968-0747
Fax Number: 815-968-8148
Web Site: http://www.piercenet.com/
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Label IT Tracker CX-Rhodamine Intracellular Nucleic Acid Localization Kit from Mirus Bio Corporation
2. Label IT Tracker Cy5 Intracellular Nucleic Acid Localization Kit from Mirus Bio Corporation
3. Label IT Tracker BiotinIntracellular Nucleic Acid Localization Kit from Mirus Bio Corporation
4. Label IT siRNA Tracker Fluorescein Kit with TransIT-siQUEST Reagent from Mirus Bio Corporation
5. Label IT siRNA Tracker CX-Rhodamine Intracellular Localization Kit with TransIT-TKO siRNA Transfection Reagent from Mirus Bio Corporation
6. Label IT siRNA Tracker CX Rhodamine Kit with TransIT-siQUEST Reagent from Mirus Bio Corporation
7. Label IT siRNA Tracker Intracellular Localization Kit with TransIT-TKO Transfection Reagent (Cy5 Labeling Kit) from Mirus Bio Corporation
8. Label IT siRNA Tracker Fluorescein Intracellular Localization Kit with TransIT-TKO siRNA Transfection Reagent from Mirus Bio Corporation
9. Label IT siRNA Tracker TM Rhodamine Kit with TransIT-siQUEST Reagent from Mirus Bio Corporation
10. Label IT siRNA Tracker Biotin Kit with TransIT-siQUEST Reagent from Mirus Bio Corporation
11. Label IT siRNA Tracker Cy3 Kit with TransIT-siQUEST Reagent from Mirus Bio Corporation
... ISO 9001:2000 Certified USDA Research Registered OLAW ... Training AALAS Certified Technicians ... GLP Documentation Upon Request Focus ... for all your custom immunology service needs. ...
... System Modules Highly ... directional cloning into advanced ... vectors For ... Cloning Systems, the modules ...
...
... antibody-based immunoaffinity matrices, such as Sepharose ... without compromising antigen-antibody reactivity, the need ... has precluded the application of these ... CHEMICON Re-Blot Plus Western Blot Mild ...
Biology Products:
(Date:9/22/2018)... ... 21, 2018 , ... VTE can present as deep vein ... significant morbidity and mortality. VTE affects between 300-600,000 Americans annually, with approximately 100,000 ... the leading cause of preventable hospital death and maternal mortality. When used with ...
(Date:9/12/2018)... ... September 10, 2018 , ... IONpathTM, a venture-backed commercial-stage ... Multiplexed Ion Beam Imaging (MIBITM) technology was accepted by the journal Cell and ... shared during a webinar presented by Dr. Mike Angelo on October 2nd, 2018. ...
(Date:9/12/2018)... , ... September 12, 2018 , ... ... spine fusion solutions announced today it has received 510(k) clearance from the U.S. ... complementing its TowerLOX® MIS Pedicle Screw System. , TowerLOX-EXT MIS Extended Tab Pedicle ...
Breaking Biology News(10 mins):
(Date:8/31/2018)... (PRWEB) , ... August 29, 2018 , ... ... Professorships at both the University of Southampton and the Institute of Industrial Science, ... intelligence at sea can dramatically accelerate the exploration and study of hard to ...
(Date:8/29/2018)... ... 28, 2018 , ... CEO and founder of VetStem Biopharma, ... on an allogeneic (donor derived) stem cell product for osteoarthritis in canines at ... September. He is also the organizer of Breakout Session 4: Practitioner Primer on ...
(Date:8/23/2018)... Nev. (PRWEB) , ... August 22, 2018 , ... ... TrialKit ™ for Android on September 1st, making it the first fully-featured app ... iOS and Android devices. Combined, iOS and Android constitute 99 percent of the ...
(Date:8/17/2018)... ... August 16, 2018 , ... KCAS ... to announce the expansion of their team. Dr. Dawn Dufield, Ph.D., joins KCAS ... innovative large molecule LC-MS/MS group that will specialize in developing quantitative LC-MS/MS assays ...
Breaking Biology Technology: