Navigation Links
ProFound Label Transfer Sulfo SBED Protein:Protein Interaction Reagent from Pierce Biotechnology, Inc.

ProductsProFound Label Transfer Sulfo SBED Protein:Protein Interaction Reagent from Pierce Biotechnology, Inc.
Company Pierce Biotechnology, Inc.
Item ProFound Label Transfer Sulfo SBED Protein:Protein Interaction Reagent
Price 
Description ProFound Label Transfer Sulfo SBED Protein:Protein Interaction Reagent

A powerful new method known as Label Transfer has rapidly gained traction as an in vitro method for protein interaction discovery. A growing number of publications feature the exclusive Pierce ProFound Sulfo-SBED Biotin Label Transfer Reagent to study a variety of issues relating to protein interactions. • The use of Sulfo-SBED has contributed greatly to published studies on protein:protein interactions. To simplify the method, Pierce has assembled the key components frequently cited in publications featuring Sulfo-SBED in the label transfer application. The resulting easy-to-use kit also contains Streptavidin-HRP for subsequent detection of the biotinylated complex or biotinylated prey protein on a Western blot. •

Label transfer is an in vitro method for discovering or verifying an interaction that was observed by way of another technique such as the in vivo yeast 2-hybrid method or by in vitro methods such as co-immunoprecipitation or pull-down assays. • The label transfer method requires a purified "bait" protein. The amine groups of the bait protein are modified via the Sulfo-NHS moiety of Sulfo-SBED. This derivatized bait protein is then incubated in the dark with a lysate or with a purified putative "prey" protein. The bait and prey complex, if formed, is then captured or trapped by exposing the sample to high-intensity UV light, which activates the phenylazide moiety of Sulfo-SBED. This photoreactive group covalently links to the bound prey protein, capturing the interacting complex. Upon reduction of this complex, the biotin label that first resided with the bait protein transfers to the prey protein. The biotin label also functions as the detection target for the bait:prey complex or the prey protein upon Western blot analysis. The resulting biotin derivatized complex or prey protein can be detected using streptavidin-HRP or an anti-biotin antibody and chemiluminescent detection. The biotin label can also be used as a handle to purify the bait:prey complex over an avidin/streptavidin/NeutrAvidin/monomeric avidin biotin-binding protein support.

  • Amine group-specific reactivity
  • Nonspecific photoreactivity
  • Thiol-cleavable disulfide (S-S) linkage
  • A Biotin handle: This biotin transfers from a labeled purified bait protein to a captured prey protein (Figure 2), which makes it especially useful for Western blot detection
Info Pierce Biotechnology, Inc.Pierce Biotechnology, Inc.
Pierce Biotechnology, Inc.
3747 N. Meridian Rd.
P.O. Box 117
Rockford, IL 61105
Customer Service: 815-968-0747
Fax Number: 815-968-8148
Web Site: http://www.piercenet.com/
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Label IT Tracker CX-Rhodamine Intracellular Nucleic Acid Localization Kit from Mirus Bio Corporation
2. Label IT Tracker Cy5 Intracellular Nucleic Acid Localization Kit from Mirus Bio Corporation
3. Label IT Tracker BiotinIntracellular Nucleic Acid Localization Kit from Mirus Bio Corporation
4. Label IT siRNA Tracker Fluorescein Kit with TransIT-siQUEST Reagent from Mirus Bio Corporation
5. Label IT siRNA Tracker CX-Rhodamine Intracellular Localization Kit with TransIT-TKO siRNA Transfection Reagent from Mirus Bio Corporation
6. Label IT siRNA Tracker CX Rhodamine Kit with TransIT-siQUEST Reagent from Mirus Bio Corporation
7. Label IT siRNA Tracker Intracellular Localization Kit with TransIT-TKO Transfection Reagent (Cy5 Labeling Kit) from Mirus Bio Corporation
8. Label IT siRNA Tracker Fluorescein Intracellular Localization Kit with TransIT-TKO siRNA Transfection Reagent from Mirus Bio Corporation
9. Label IT siRNA Tracker TM Rhodamine Kit with TransIT-siQUEST Reagent from Mirus Bio Corporation
10. Label IT siRNA Tracker Biotin Kit with TransIT-siQUEST Reagent from Mirus Bio Corporation
11. Label IT siRNA Tracker Cy3 Kit with TransIT-siQUEST Reagent from Mirus Bio Corporation
Sheep polyclonal to Salmonella Thomasville ( Abpromise for all tested applications). Antigen: Native Salmonella thomasville...
anti-achaete...
... Rabbit polyclonal to Oct6 ( Abpromise for ... Synthetic peptide conjugated to KLH derived from within ... (Note: the amino acid sequence is proprietary) ... Entrez Gene ID: 5453 ...
... Super-Dark, Super-Small! Capture high-resolution chemiluminescent ... Imaging System. High-sensitivity camera is cooled ... and wide dynamic range for high ... time. The light-tight cabinet provides optimum ...
Biology Products:
(Date:4/4/2017)... 2017   EyeLock LLC , a leader of ... States Patent and Trademark Office (USPTO) has issued U.S. ... of an iris image with a face image acquired ... company,s 45 th issued patent. ... given the multi-modal biometric capabilities that have recently come ...
(Date:3/30/2017)... , March 30, 2017 Trends, opportunities ... (physiological and behavioral), by technology (fingerprint, AFIS, iris recognition, ... recognition, and others), by end use industry (government and ... immigration, financial and banking, and others), and by region ... , Asia Pacific , and ...
(Date:3/24/2017)... Controller General of Immigration from Maldives Mr. Mohamed Anwar and ... international IAIR Award for the most innovative high security ePassport and eGates  ... ... Maldives Immigration Controller General, Mr. Mohamed Anwar ... right) have received the IAIR award for the "Most innovative high security ...
Breaking Biology News(10 mins):
(Date:10/11/2017)... and LAGUNA HILLS, Calif. , Oct. 11, ... Research, London (ICR) and University of ... SkylineDx,s prognostic tool to risk-stratify patients with multiple myeloma (MM), ... nine . The University of Leeds ... funded by Myeloma UK, and ICR will perform the testing ...
(Date:10/11/2017)... ... October 11, 2017 , ... A new study ... in frozen and fresh in vitro fertilization (IVF) transfer cycles. The ... IVF success. , After comparing the results from the fresh and frozen transfer ...
(Date:10/10/2017)... ... October 10, 2017 , ... San Diego-based team building and ... rebranding initiative announced today. The bold new look is part of a transformation ... moves into a significant growth period. , It will also expand its service offering ...
(Date:10/10/2017)... PA (PRWEB) , ... October 10, 2017 , ... ... City Science Center’s FirstHand program has won a US2020 STEM Mentoring Award. Representatives ... the award for Excellence in Volunteer Experience from US2020. , US2020’s mission is ...
Breaking Biology Technology: