Navigation Links
Polyclonal Antibody Production from Agro-Bio

ProductsPolyclonal Antibody Production from Agro-Bio
Company Agro-Bio
Item Polyclonal Antibody Production
Price 
Description Conjugated peptides, recombinant or native proteins are used to immunize rabbits.
Agro-Bio has developed an immunization protocol optimized in time, quantity of antigen and quality of the antibodies obtained. We recommend the use of 2 animals per protocol in order to avoid animal variability. The average serum yield on a 77-day protocol is approximately 75 mL per rabbit. 150 to 250 g immunogen is used for each immunization. Schedule: Day 0 : Bleed 2 mL per rabbit (pre-immune serum) Immunize with antigen in Complete Freund's Adjuvant (CFA)
Day 14 : Immunize with antigen in Incomplete Freund's Adjuvant (IFA)
Day 28 : Immunize with antigen in Incomplete Freund's Adjuvant (IFA)
Day 42 : Immunize with antigen in Incomplete Freund's Adjuvant (IFA)
Day 49 : 1st production bleed 20-25 mL
Day 56 : Immunize with antigen in Incomplete Freund's Adjuvant (IFA)
Day 63 : 2nd production bleed 20-25 mL
Day 77 : 3rd production bleed 20-25 mL

The rabbits can be maintained on a two-week cycle of immunization and bleeds as required by the project.

Additional services:
Peptide synthesis and conjugation
ELISA test
Antibody purification on protein A/G or by affinity on antigen

Chickens are an excellent host for producing antibodies. In most cases high titer polyclonal chicken antibodies can be obtained in one month immunization protocol. Conjugated peptides, recombinant or native proteins are used to immunize chickens.

Schedule:
Day 0: Purified the IgY from 3 pre-immune egg yolk per chicken (serve as negative controls). Immunize with antigen in Complete Freund's Adjuvant (CFA)
Day 10: Immunize with antigen in Incomplete Freund's Adjuvant (IFA)
Day 20: Immunize with antigen in IFA
Day 35: Purified antibody (>90% pure) from 6 immunized egg yolk per chicken

Additional services:
Peptide synthesis and conjugation
ELISA test
Antibody purification by affinity on antigen in order to obtain specific IgY

Conjugated peptides, recombinant or native proteins are used to immunize goat. Agro-Bio has developed an immunization protocol optimized in time, quantity of antigen and quality of the antibodies obtained. The average serum yield on a 77-day protocol is approximately 300 mL per goat. 150 to 250 g immunogen is used for each immunization (5 immunizations/ goat with a 77-days protocol).

Schedule:
Day 0 : Pre-immunization bleed Immunize with antigen in Complete Freund's Adjuvant (CFA)
Day 14 : Immunize with antigen in Incomplete Freund's Adjuvant (IFA)
Day 28 : Immunize with antigen in Incomplete Freund's Adjuvant (IFA)
Day 42 : Immunize with antigen in Incomplete Freund's Adjuvant (IFA)
Day 56 : 1st production bleed 150 mL
Day 63 : Immunize with antigen in Incomplete Freund's Adjuvant (IFA)
Day 77 : 2nd production bleed 150 mL

Goat can be maintained on a three-week cycle of immunization and bleeds as required by the project.

Additional services:
Peptide synthesis and conjugation
ELISA test
Antibody purification on protein A/G or by affinity on antigen

Info Agro-BioAgro-Bio
La Chavannerie
Rue Denis Papin
45 240 La Fert St Aubin
Customer Service: +33 (0)2 38 64 83 50
Fax Number: +33 (0)2 38 64 83 59
Web Site: http://www.agro-bio.fr
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Goat Anti-TGase3 (S-20) Polyclonal Antibody, Unconjugated from Santa Cruz Biotechnology, Inc.
2. Rabbit Anti-Aph-1aL, S Loop (92-115) Polyclonal Antibody, Unconjugated from Covance Research Products, Inc
3. Goat Anti-SCCRO (S-17) Polyclonal Antibody, Unconjugated from Santa Cruz Biotechnology, Inc.
4. Rabbit Anti-Human Vitamin K-dependent Protein S (PROS1) Polyclonal Antibody, Unconjugated from Lifespan Biosciences
5. Goat Anti-ZBP-89 (S-15) Polyclonal Antibody, Unconjugated from Santa Cruz Biotechnology, Inc.
6. Goat Anti-XPLAC (S-17) Polyclonal Antibody, Unconjugated from Santa Cruz Biotechnology, Inc.
7. Anti-JIP 1 / 2 (SH3) Polyclonal Antibody, Unconjugated, Clone ZMD.177 from Invitrogen
8. Rabbit Anti-Human Sirtuin (silent mating type information regulation 2, S.cerevisiae, homolog) 7 (SIRT7) Polyclonal Antibody, Unconjugated from Lifespan Biosciences
9. Goat Anti-Human RORgamma (S-14) Polyclonal Antibody, Unconjugated from Santa Cruz Biotechnology, Inc.
10. Rabbit Anti-Esa1 Polyclonal Antibody, Unconjugated from Abcam
11. Rabbit Anti-Human Rad51 Polyclonal Antibody, Unconjugated from Abcam
...
The S. cerevisiae Hansen strains are the parental yeast strains from which the deletions were derived....
The DNA DipStick Kit is ideal for estimating nucleic acid concentrations and approximate yields. Works with ss- and ds-DNA, RNA, and oligonucleotides at concentrations as low as 0.1 ng/l....
... The NEW HandyStep electronic from BRAND ... pipetting. It is the only electronic, ... standard syringe tips, including BRAND PD-Tip ... Plus, Fisherbrand Dispenser Tips,and VWRbrand Combi-Syringes. ...
Biology Products:
(Date:7/9/2014)... Calif. Jason Stajich , an associate professor ... University of California, Riverside, has been awarded the 2014 ... America, a scientific society dedicated to advancing the science ... kinds including mushrooms, molds, truffles, yeasts, lichens, plant pathogens, ... and each year recognizes an outstanding early-career mycologist. Stajich ...
(Date:7/9/2014)... species, selection for traits that are attractive to the ... opportunities to mate, the tail of male peacocks being ... California, Riverside have now found that sexual selection and ... linked. Describing the life histories of more than 150 ... found that species with placentas tend to have males ...
(Date:7/9/2014)... studying critically ill children with traumatic injuries have ... are likely to develop a hospital-acquired infection. The ... and published online in June in the journal ... efforts that could lead to the clinical implementation ... prevent or reverse immune system damage following critical ...
Breaking Biology News(10 mins):UC Riverside plant pathologist receives national recognition 2Biologists link sexual selection and placenta formation 2Biologists link sexual selection and placenta formation 3Biologists link sexual selection and placenta formation 4Immune function predicts infection risk among child trauma patients 2Immune function predicts infection risk among child trauma patients 3
... tree ring-based reconstruction of an extinct polar forest,s ... impact of Lake St. Martin bolide on groundwater ... boundary; radar images of volcanic and impact deposits ... into the atmosphere; use of charred plants in ...
... -- The National Academy of Sciences (NAS) will honor ... the areas of biology, chemistry, solar physics, ecology, mathematics, ... awards and recipients for 2008 are: ARCTOWSKI MEDAL ... well as a gift of $60,000 to an institution ...
... Climate Change for the Baltic Sea Basin (BACC), the book ... and possible future climate change in the region, which extends ... mid-Europe. One of the key findings is that air temperatures ... five degrees Celsius from now to 2100. More than 80 ...
Cached Biology News:February Geology and GSA Today media highlights 2February Geology and GSA Today media highlights 3February Geology and GSA Today media highlights 4February Geology and GSA Today media highlights 5February Geology and GSA Today media highlights 6February Geology and GSA Today media highlights 7February Geology and GSA Today media highlights 8February Geology and GSA Today media highlights 9February Geology and GSA Today media highlights 10Academy honors 13 for major contributions to science 2Academy honors 13 for major contributions to science 3Academy honors 13 for major contributions to science 4Academy honors 13 for major contributions to science 5Climate change in the Baltic Sea basin -- past, present and future 2Climate change in the Baltic Sea basin -- past, present and future 3
(Date:1/15/2014)... TaiGen Biotechnology Company, Limited ("TaiGen") today announced that they ... Russian pharmaceutical company, to develop and commercialize nemonoxacin (Taigexyn ... , Turkey and other members ... antibiotic for the treatment of bacterial infections including those ...
(Date:1/14/2014)... , Jan. 14, 2014  3D Communications, a leading provider of strategic ... business, and media events in the United States ... associate Virginia Cox , JD, is returning to the firm,s ... Cox re-joins 3D after more than two years of service ...
(Date:1/14/2014)... BETHESDA, Md. , Jan. 14, 2014  Organovo ... institutes from the National Institutes of Health (NIH) to ... more effective treatments to patients on a faster timeline. ... Translational Sciences (NCATS) and the National Eye Institute (NEI) ...
(Date:1/14/2014)... 14, 2014 Global Record Systems, ... information technology solutions for patients, physicians, the biopharmaceutical ... announced today the signing of a three-year Research ... Drug Administration (FDA). This initiative is designed ...
Breaking Biology Technology:TaiGen Biotechnology Signed Exclusive License Agreement with R-Pharm for Nemonoxacin (Taigexyn(R)) 2TaiGen Biotechnology Signed Exclusive License Agreement with R-Pharm for Nemonoxacin (Taigexyn(R)) 3TaiGen Biotechnology Signed Exclusive License Agreement with R-Pharm for Nemonoxacin (Taigexyn(R)) 4Former FDA Associate Commissioner Returns To 3D Communications 2Organovo Announces Collaboration with National Institutes of Health 2Organovo Announces Collaboration with National Institutes of Health 3Global Record Systems Announces Research Collaboration Agreement with FDA to Create a Novel “Big Data” Paradigm for Collection of Patient Safety and Outcomes Information 2