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Polyclonal Antibody Production from Agro-Bio

ProductsPolyclonal Antibody Production from Agro-Bio
Company Agro-Bio
Item Polyclonal Antibody Production
Price 
Description Conjugated peptides, recombinant or native proteins are used to immunize rabbits.
Agro-Bio has developed an immunization protocol optimized in time, quantity of antigen and quality of the antibodies obtained. We recommend the use of 2 animals per protocol in order to avoid animal variability. The average serum yield on a 77-day protocol is approximately 75 mL per rabbit. 150 to 250 g immunogen is used for each immunization. Schedule: Day 0 : Bleed 2 mL per rabbit (pre-immune serum) Immunize with antigen in Complete Freund's Adjuvant (CFA)
Day 14 : Immunize with antigen in Incomplete Freund's Adjuvant (IFA)
Day 28 : Immunize with antigen in Incomplete Freund's Adjuvant (IFA)
Day 42 : Immunize with antigen in Incomplete Freund's Adjuvant (IFA)
Day 49 : 1st production bleed 20-25 mL
Day 56 : Immunize with antigen in Incomplete Freund's Adjuvant (IFA)
Day 63 : 2nd production bleed 20-25 mL
Day 77 : 3rd production bleed 20-25 mL

The rabbits can be maintained on a two-week cycle of immunization and bleeds as required by the project.

Additional services:
Peptide synthesis and conjugation
ELISA test
Antibody purification on protein A/G or by affinity on antigen

Chickens are an excellent host for producing antibodies. In most cases high titer polyclonal chicken antibodies can be obtained in one month immunization protocol. Conjugated peptides, recombinant or native proteins are used to immunize chickens.

Schedule:
Day 0: Purified the IgY from 3 pre-immune egg yolk per chicken (serve as negative controls). Immunize with antigen in Complete Freund's Adjuvant (CFA)
Day 10: Immunize with antigen in Incomplete Freund's Adjuvant (IFA)
Day 20: Immunize with antigen in IFA
Day 35: Purified antibody (>90% pure) from 6 immunized egg yolk per chicken

Additional services:
Peptide synthesis and conjugation
ELISA test
Antibody purification by affinity on antigen in order to obtain specific IgY

Conjugated peptides, recombinant or native proteins are used to immunize goat. Agro-Bio has developed an immunization protocol optimized in time, quantity of antigen and quality of the antibodies obtained. The average serum yield on a 77-day protocol is approximately 300 mL per goat. 150 to 250 g immunogen is used for each immunization (5 immunizations/ goat with a 77-days protocol).

Schedule:
Day 0 : Pre-immunization bleed Immunize with antigen in Complete Freund's Adjuvant (CFA)
Day 14 : Immunize with antigen in Incomplete Freund's Adjuvant (IFA)
Day 28 : Immunize with antigen in Incomplete Freund's Adjuvant (IFA)
Day 42 : Immunize with antigen in Incomplete Freund's Adjuvant (IFA)
Day 56 : 1st production bleed 150 mL
Day 63 : Immunize with antigen in Incomplete Freund's Adjuvant (IFA)
Day 77 : 2nd production bleed 150 mL

Goat can be maintained on a three-week cycle of immunization and bleeds as required by the project.

Additional services:
Peptide synthesis and conjugation
ELISA test
Antibody purification on protein A/G or by affinity on antigen

Info Agro-BioAgro-Bio
La Chavannerie
Rue Denis Papin
45 240 La Fert St Aubin
Customer Service: +33 (0)2 38 64 83 50
Fax Number: +33 (0)2 38 64 83 59
Web Site: http://www.agro-bio.fr
NOTE: Price information is approximate list price and actual prices may vary.

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