Navigation Links
Polyclonal Antibody Production from Agro-Bio

ProductsPolyclonal Antibody Production from Agro-Bio
Company Agro-Bio
Item Polyclonal Antibody Production
Price 
Description Conjugated peptides, recombinant or native proteins are used to immunize rabbits.
Agro-Bio has developed an immunization protocol optimized in time, quantity of antigen and quality of the antibodies obtained. We recommend the use of 2 animals per protocol in order to avoid animal variability. The average serum yield on a 77-day protocol is approximately 75 mL per rabbit. 150 to 250 g immunogen is used for each immunization. Schedule: Day 0 : Bleed 2 mL per rabbit (pre-immune serum) Immunize with antigen in Complete Freund's Adjuvant (CFA)
Day 14 : Immunize with antigen in Incomplete Freund's Adjuvant (IFA)
Day 28 : Immunize with antigen in Incomplete Freund's Adjuvant (IFA)
Day 42 : Immunize with antigen in Incomplete Freund's Adjuvant (IFA)
Day 49 : 1st production bleed 20-25 mL
Day 56 : Immunize with antigen in Incomplete Freund's Adjuvant (IFA)
Day 63 : 2nd production bleed 20-25 mL
Day 77 : 3rd production bleed 20-25 mL

The rabbits can be maintained on a two-week cycle of immunization and bleeds as required by the project.

Additional services:
Peptide synthesis and conjugation
ELISA test
Antibody purification on protein A/G or by affinity on antigen

Chickens are an excellent host for producing antibodies. In most cases high titer polyclonal chicken antibodies can be obtained in one month immunization protocol. Conjugated peptides, recombinant or native proteins are used to immunize chickens.

Schedule:
Day 0: Purified the IgY from 3 pre-immune egg yolk per chicken (serve as negative controls). Immunize with antigen in Complete Freund's Adjuvant (CFA)
Day 10: Immunize with antigen in Incomplete Freund's Adjuvant (IFA)
Day 20: Immunize with antigen in IFA
Day 35: Purified antibody (>90% pure) from 6 immunized egg yolk per chicken

Additional services:
Peptide synthesis and conjugation
ELISA test
Antibody purification by affinity on antigen in order to obtain specific IgY

Conjugated peptides, recombinant or native proteins are used to immunize goat. Agro-Bio has developed an immunization protocol optimized in time, quantity of antigen and quality of the antibodies obtained. The average serum yield on a 77-day protocol is approximately 300 mL per goat. 150 to 250 g immunogen is used for each immunization (5 immunizations/ goat with a 77-days protocol).

Schedule:
Day 0 : Pre-immunization bleed Immunize with antigen in Complete Freund's Adjuvant (CFA)
Day 14 : Immunize with antigen in Incomplete Freund's Adjuvant (IFA)
Day 28 : Immunize with antigen in Incomplete Freund's Adjuvant (IFA)
Day 42 : Immunize with antigen in Incomplete Freund's Adjuvant (IFA)
Day 56 : 1st production bleed 150 mL
Day 63 : Immunize with antigen in Incomplete Freund's Adjuvant (IFA)
Day 77 : 2nd production bleed 150 mL

Goat can be maintained on a three-week cycle of immunization and bleeds as required by the project.

Additional services:
Peptide synthesis and conjugation
ELISA test
Antibody purification on protein A/G or by affinity on antigen

Info Agro-BioAgro-Bio
La Chavannerie
Rue Denis Papin
45 240 La Fert St Aubin
Customer Service: +33 (0)2 38 64 83 50
Fax Number: +33 (0)2 38 64 83 59
Web Site: http://www.agro-bio.fr
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Goat Anti-TGase3 (S-20) Polyclonal Antibody, Unconjugated from Santa Cruz Biotechnology, Inc.
2. Rabbit Anti-Aph-1aL, S Loop (92-115) Polyclonal Antibody, Unconjugated from Covance Research Products, Inc
3. Goat Anti-SCCRO (S-17) Polyclonal Antibody, Unconjugated from Santa Cruz Biotechnology, Inc.
4. Rabbit Anti-Human Vitamin K-dependent Protein S (PROS1) Polyclonal Antibody, Unconjugated from Lifespan Biosciences
5. Goat Anti-ZBP-89 (S-15) Polyclonal Antibody, Unconjugated from Santa Cruz Biotechnology, Inc.
6. Goat Anti-XPLAC (S-17) Polyclonal Antibody, Unconjugated from Santa Cruz Biotechnology, Inc.
7. Anti-JIP 1 / 2 (SH3) Polyclonal Antibody, Unconjugated, Clone ZMD.177 from Invitrogen
8. Rabbit Anti-Human Sirtuin (silent mating type information regulation 2, S.cerevisiae, homolog) 7 (SIRT7) Polyclonal Antibody, Unconjugated from Lifespan Biosciences
9. Goat Anti-Human RORgamma (S-14) Polyclonal Antibody, Unconjugated from Santa Cruz Biotechnology, Inc.
10. Rabbit Anti-Esa1 Polyclonal Antibody, Unconjugated from Abcam
11. Rabbit Anti-Human Rad51 Polyclonal Antibody, Unconjugated from Abcam
Ly-49C and Ly-49I Biotin Anti-Mouse clone 5E6, Isotype Mouse IgG 2a , kappa, 0.1 mg Consult technical datasheet for details....
Dog polyclonal to Porcine Pancreatic Islets of Langerhans Cells ( Abpromise for all tested applications). Antigen: Tissue / cell preparation (Pig): Sonicated crude membrane fraction of porcine p...
Anti-Cohesin (Scc1/Rad21) Monoclonal Antibody Research Focus: signal transduction Storage: -20C Shipping Temperature: +4C...
Proline-serine-threonine phosphatase-interacting protein 2...
Biology Products:
(Date:12/5/2014)...  Tute Genomics, a leader in cloud-based solutions for ... funding led by UK-based Eurovestech. Peak Ventures and a ... round. "We are at a pivotal time ... and seeks new approaches for the diagnosis, treatment and ... MBA, and CEO of Tute Genomics. "One of the ...
(Date:11/21/2014)... YORK , Nov. 19, 2014  Earlier this ... at New York College, and one of the most ... biosensor signals that are transmitted from Smartphones to third ... were Smartphones and has one of the earliest known ... discussing its usage in the military, child care, elder ...
(Date:11/18/2014)... 2014   News Highlights: ... develop The Partners Data Lake, an agile data ... Lake will allow researcher and clinicians to explore ... the lives of patients , The Partners ... across the Partners system, breaking down physical barriers ...
Breaking Biology News(10 mins):Tute Genomics Raises $2.3 Million to Scale Up Cloud-Based Genome Analytics and Accelerate Personalized Medicine 2Tute Genomics Raises $2.3 Million to Scale Up Cloud-Based Genome Analytics and Accelerate Personalized Medicine 3Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 2Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 3Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 4Partners HealthCare and EMC Unite to Solve Health Care's Toughest Challenges Using Big Data 2Partners HealthCare and EMC Unite to Solve Health Care's Toughest Challenges Using Big Data 3Partners HealthCare and EMC Unite to Solve Health Care's Toughest Challenges Using Big Data 4Partners HealthCare and EMC Unite to Solve Health Care's Toughest Challenges Using Big Data 5
... Washington, D.C. (October 5, 2010) -- Carbon nanotubes -- ... in diameter -- have many potential uses in nanotechnology, ... of nanotubes depend on their structure, and scientists as ... determined during the initial formation -- or growth -- ...
... BOZEMAN, Mont. -- Montana State University researchers have discovered a ... geothermal vents at the bottom of Yellowstone Lake. A ... in an area where the water is inky black, about ... poisons, the researchers reported in the September issue of Geobiology. ...
... toxic pollution falling onto Puget Sound,s waters has decreased ... below earlier estimates, according to a region-wide study. Despite ... like Tacoma still have the Puget,s Sound,s highest air-deposited ... of Energy,s Pacific Northwest National Laboratory and Texas A&M ...
Cached Biology News:The effects of hydrogen on growing carbon nanotubes 2Montana State team finds rare oasis of life on floor of Yellowstone Lake 2Montana State team finds rare oasis of life on floor of Yellowstone Lake 3Montana State team finds rare oasis of life on floor of Yellowstone Lake 4The sky is falling (less) onto Puget Sound 2The sky is falling (less) onto Puget Sound 3The sky is falling (less) onto Puget Sound 4
(Date:12/19/2014)... The empty capsules market is experiencing growth ... technological innovations in the empty capsules market. The ... growth of the empty capsules market. , Get ... of Report , The report will enrich both ... to gauge the pulse of the market which ...
(Date:12/19/2014)... 18, 2014 Relmada Therapeutics, Inc. (OTCQB: RLMD), ... of chronic pain, announced the results of its annual ... was a transformational year in Relmada,s history and it ... company," said Sergio Traversa , chief executive officer ... on key financial, human resource and clinical development objectives ...
(Date:12/17/2014)... RIDGE, N.J. (PRWEB) December 17, 2014 ... (Euronext: IPN; ADR: IPSEY), today ... mg (referred to as Somatuline®) was approved by ... the treatment of gastroenteropancreatic neuroendocrine tumors (GEP-NETs) in ... locally advanced or metastatic disease to improve progression-free ...
(Date:12/17/2014)... 2014 Audacity, a leading California-based health ... of the Huntsworth Health Group, is proud to announce ... In her role, Gonzales will serve as Senior Vice ... honored to welcome a talent of Jamie’s caliber to ... Fraikin. “She is a proven healthcare innovation leader and ...
Breaking Biology Technology:Empty Capsules Market Hit to Reach $1,820 Million by 2019 - New Research Report by MarketsandMarkets 2Empty Capsules Market Hit to Reach $1,820 Million by 2019 - New Research Report by MarketsandMarkets 3Empty Capsules Market Hit to Reach $1,820 Million by 2019 - New Research Report by MarketsandMarkets 4Relmada Therapeutics Announces Results of Annual Shareholders Meeting 2Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 2Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 3Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 4Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 5Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 6Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 7Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 8Audacity Welcomes Healthcare Communications Expert Jamie Gonzales as Senior Vice President of Client Services 2