Navigation Links
Polyclonal Antibody Production from Agro-Bio

ProductsPolyclonal Antibody Production from Agro-Bio
Company Agro-Bio
Item Polyclonal Antibody Production
Price 
Description Conjugated peptides, recombinant or native proteins are used to immunize rabbits.
Agro-Bio has developed an immunization protocol optimized in time, quantity of antigen and quality of the antibodies obtained. We recommend the use of 2 animals per protocol in order to avoid animal variability. The average serum yield on a 77-day protocol is approximately 75 mL per rabbit. 150 to 250 g immunogen is used for each immunization. Schedule: Day 0 : Bleed 2 mL per rabbit (pre-immune serum) Immunize with antigen in Complete Freund's Adjuvant (CFA)
Day 14 : Immunize with antigen in Incomplete Freund's Adjuvant (IFA)
Day 28 : Immunize with antigen in Incomplete Freund's Adjuvant (IFA)
Day 42 : Immunize with antigen in Incomplete Freund's Adjuvant (IFA)
Day 49 : 1st production bleed 20-25 mL
Day 56 : Immunize with antigen in Incomplete Freund's Adjuvant (IFA)
Day 63 : 2nd production bleed 20-25 mL
Day 77 : 3rd production bleed 20-25 mL

The rabbits can be maintained on a two-week cycle of immunization and bleeds as required by the project.

Additional services:
Peptide synthesis and conjugation
ELISA test
Antibody purification on protein A/G or by affinity on antigen

Chickens are an excellent host for producing antibodies. In most cases high titer polyclonal chicken antibodies can be obtained in one month immunization protocol. Conjugated peptides, recombinant or native proteins are used to immunize chickens.

Schedule:
Day 0: Purified the IgY from 3 pre-immune egg yolk per chicken (serve as negative controls). Immunize with antigen in Complete Freund's Adjuvant (CFA)
Day 10: Immunize with antigen in Incomplete Freund's Adjuvant (IFA)
Day 20: Immunize with antigen in IFA
Day 35: Purified antibody (>90% pure) from 6 immunized egg yolk per chicken

Additional services:
Peptide synthesis and conjugation
ELISA test
Antibody purification by affinity on antigen in order to obtain specific IgY

Conjugated peptides, recombinant or native proteins are used to immunize goat. Agro-Bio has developed an immunization protocol optimized in time, quantity of antigen and quality of the antibodies obtained. The average serum yield on a 77-day protocol is approximately 300 mL per goat. 150 to 250 g immunogen is used for each immunization (5 immunizations/ goat with a 77-days protocol).

Schedule:
Day 0 : Pre-immunization bleed Immunize with antigen in Complete Freund's Adjuvant (CFA)
Day 14 : Immunize with antigen in Incomplete Freund's Adjuvant (IFA)
Day 28 : Immunize with antigen in Incomplete Freund's Adjuvant (IFA)
Day 42 : Immunize with antigen in Incomplete Freund's Adjuvant (IFA)
Day 56 : 1st production bleed 150 mL
Day 63 : Immunize with antigen in Incomplete Freund's Adjuvant (IFA)
Day 77 : 2nd production bleed 150 mL

Goat can be maintained on a three-week cycle of immunization and bleeds as required by the project.

Additional services:
Peptide synthesis and conjugation
ELISA test
Antibody purification on protein A/G or by affinity on antigen

Info Agro-BioAgro-Bio
La Chavannerie
Rue Denis Papin
45 240 La Fert St Aubin
Customer Service: +33 (0)2 38 64 83 50
Fax Number: +33 (0)2 38 64 83 59
Web Site: http://www.agro-bio.fr
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Goat Anti-TGase3 (S-20) Polyclonal Antibody, Unconjugated from Santa Cruz Biotechnology, Inc.
2. Rabbit Anti-Aph-1aL, S Loop (92-115) Polyclonal Antibody, Unconjugated from Covance Research Products, Inc
3. Goat Anti-SCCRO (S-17) Polyclonal Antibody, Unconjugated from Santa Cruz Biotechnology, Inc.
4. Rabbit Anti-Human Vitamin K-dependent Protein S (PROS1) Polyclonal Antibody, Unconjugated from Lifespan Biosciences
5. Goat Anti-ZBP-89 (S-15) Polyclonal Antibody, Unconjugated from Santa Cruz Biotechnology, Inc.
6. Goat Anti-XPLAC (S-17) Polyclonal Antibody, Unconjugated from Santa Cruz Biotechnology, Inc.
7. Anti-JIP 1 / 2 (SH3) Polyclonal Antibody, Unconjugated, Clone ZMD.177 from Invitrogen
8. Rabbit Anti-Human Sirtuin (silent mating type information regulation 2, S.cerevisiae, homolog) 7 (SIRT7) Polyclonal Antibody, Unconjugated from Lifespan Biosciences
9. Goat Anti-Human RORgamma (S-14) Polyclonal Antibody, Unconjugated from Santa Cruz Biotechnology, Inc.
10. Rabbit Anti-Esa1 Polyclonal Antibody, Unconjugated from Abcam
11. Rabbit Anti-Human Rad51 Polyclonal Antibody, Unconjugated from Abcam
Luminometers...
cDNA Size Fractionation Columns offer an efficient and convenient method for size-fractioning double-stranded cDNA....
Homo sapiens inhibin, beta B (activin AB beta polypeptide) Antigen: Recombinant Protein(142~254aa)...
Wheat Germ Agglutinin (WGA) PS SPA Imaging Bead, 2 g. Category: Leadseeker Beads....
Biology Products:
(Date:4/28/2016)... First quarter 2016:   , Revenues amounted ... quarter of 2015 The gross margin was 49% (27) ... the operating margin was 40% (-13) Earnings per share ... operations was SEK 249.9 M (21.2) , Outlook   ... M. The operating margin for 2016 is estimated to ...
(Date:4/15/2016)... 2016 Research and Markets has ... Market 2016-2020,"  report to their offering.  , ... ,The global gait biometrics market is expected to ... period 2016-2020. Gait analysis generates multiple ... used to compute factors that are not or ...
(Date:3/29/2016)... , March 29, 2016 LegacyXChange, ... LegacyXChange "LEGX" and SelectaDNA/CSI Protect are pleased to announce ... used in a variety of writing instruments, ensuring athletes ... originally created collectibles from athletes on LegacyXChange will be ... of the DNA. Bill Bollander , ...
Breaking Biology News(10 mins):
(Date:6/24/2016)... , June 24, 2016 Epic ... sensitively detects cancers susceptible to PARP inhibitors by ... tumor cells (CTCs). The new test has already ... therapeutics in multiple cancer types. Over ... DNA damage response pathways, including PARP, ATM, ATR, ...
(Date:6/24/2016)... ... ... Researchers at the Universita Politecnica delle Marche in Ancona combed medical journal articles ... findings are the subject of a new article on the Surviving Mesothelioma website. ... blood, lung fluid or tissue of mesothelioma patients that can help point doctors to ...
(Date:6/23/2016)... ... June 23, 2016 , ... UAS LifeSciences, one of ... their brand, UP4™ Probiotics, into Target stores nationwide. The company, which has been ... Target to its list of well-respected retailers. This list includes such fine stores ...
(Date:6/23/2016)... 23, 2016 /PRNewswire/ - FACIT has announced the ... biotechnology company, Propellon Therapeutics Inc. ("Propellon" or ... of a portfolio of first-in-class WDR5 inhibitors for ... as WDR5 represent an exciting class of therapies, ... medicine for cancer patients. Substantial advances have been ...
Breaking Biology Technology: