| Products | PolarScreen Progesterone Receptor Competitor Assay Kit, Far Red from Molecular Probes (Invitrogen) | |
| Company | Molecular Probes (Invitrogen) | |
| Item | PolarScreen Progesterone Receptor Competitor Assay Kit, Far Red | |
| Price | ||
| Description | PolarScreen Progesterone Receptor (PR) Competitor Assay Kits are ideal for studying or screening novel progesterone receptor binding compounds using fluorescence polarization (FP). The homogenous assay utilizes a fusion of glutathione transferase to the ligand binding domain of human progesterone receptor [PR-LBD(GST)] and a proprietary, fluorescently tagged progesterone ligand, Fluormone PL Green, PL Red, or PL Far Red. Intrinsic background fluorescence from library compounds is substantially reduced utilizing the red-shifted Fluormone ligands.To assay:PR-LBD (GST) added to Fluormone PL Green, PL Red, or PL Far Red in the absence of competitor will form complexes that result in a high polarization value. Test compounds that compete for binding to PR-LBD (GST) prevent the formation of a Fluormone/PR-LBD (GST) complex, resulting in a lower polarization value. The shift in polarization in the presence of test compounds is used to determine the relative affinity of test compounds for PR-LBD (GST) (Figure 1, Table 1). The PolarScreen Progesterone Receptor Competitor Assay Kits contain partially purified PR-LBD (GST) protein; Fluormone PL Green (green assay kit), PL Red (red assay kit) or PL Far Red (far red assay kit) proprietary fluorescent progesterone ligands; and proprietary buffer. Store components as indicated in the assay protocol (-80C, -20C, or +4C). | |
| Info | Molecular Probes (Invitrogen)PO Box 22010 Eugene, OR 97402-0469
Fax Number: 1-541-344-6504 Web Site: http://probes.invitrogen.com |