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PolarScreen Progesterone Receptor Competitor Assay Kit, Far Red from Molecular Probes (Invitrogen)

ProductsPolarScreen Progesterone Receptor Competitor Assay Kit, Far Red from Molecular Probes (Invitrogen)
Company Molecular Probes (Invitrogen)
Item PolarScreen Progesterone Receptor Competitor Assay Kit, Far Red
Description PolarScreen Progesterone Receptor (PR) Competitor Assay Kits are ideal for studying or screening novel progesterone receptor binding compounds using fluorescence polarization (FP). The homogenous assay utilizes a fusion of glutathione transferase to the ligand binding domain of human progesterone receptor [PR-LBD(GST)] and a proprietary, fluorescently tagged progesterone ligand, Fluormone PL Green, PL Red, or PL Far Red. Intrinsic background fluorescence from library compounds is substantially reduced utilizing the red-shifted Fluormone ligands.To assay:PR-LBD (GST) added to Fluormone PL Green, PL Red, or PL Far Red in the absence of competitor will form complexes that result in a high polarization value. Test compounds that compete for binding to PR-LBD (GST) prevent the formation of a Fluormone/PR-LBD (GST) complex, resulting in a lower polarization value. The shift in polarization in the presence of test compounds is used to determine the relative affinity of test compounds for PR-LBD (GST) (Figure 1, Table 1).

The PolarScreen Progesterone Receptor Competitor Assay Kits contain partially purified PR-LBD (GST) protein; Fluormone PL Green (green assay kit), PL Red (red assay kit) or PL Far Red (far red assay kit) proprietary fluorescent progesterone ligands; and proprietary buffer. Store components as indicated in the assay protocol (-80C, -20C, or +4C).
Info Molecular Probes (Invitrogen)Molecular Probes (Invitrogen)
PO Box 22010
Eugene, OR 97402-0469

Call Molecular Probes (Invitrogen) to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-541-359-2658
Customer Service: 1-541-465-8300
Fax Number: 1-541-344-6504
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NOTE: Price information is approximate list price and actual prices may vary.

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