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PolarScreen PPARGamma-Competitor Assay Kit, Red from Molecular Probes (Invitrogen)

ProductsPolarScreen PPARGamma-Competitor Assay Kit, Red from Molecular Probes (Invitrogen)
Company Molecular Probes (Invitrogen)
Item PolarScreen PPARGamma-Competitor Assay Kit, Red
Description The PolarScreen Peroxisome Proliferator-Activated Receptor-gamma (PPARGamma) Competitor Assay Kits provide a sensitive and efficient method for studying and screening potential PPARGamma ligands using fluorescence polarization (FP). The kit contains human PPARGamma-Ligand binding domain (PPARGamma-LBD) with an N-terminal His-tag and a novel, selective fluorescent PPARGamma ligand: Fluormone PPARGamma Green (green assay kit) or Fluormone PPARGamma Red (red assay kit) in a homogenous mix-and-read assay format.To assay: PPARGamma-LBD is added to FluormonePPARGamma Green or Fluormone PPARGamma Red to form a PPARGamma-LBD/Fluormone complex. Binding of the PPARGamma-LBD to the Fluormone ligand results in a high polarization value. This complex is then added to individual test compounds in microwell plates. Competitors displace the Fluormone ligand from the PPARGamma-LBD/Fluormone complex, causing the Fluormone ligand to tumble rapidly during its fluorescent lifetime, resulting in a low polarization value. Non-competitors will not displace the Fluormone ligand from the complex, so the polarization value remains high. The shift in polarization value in the presence of test compounds is used to determine the relative affinity of test compounds for PPARGamma-LBD.

The PolarScreen PPARGamma Competitor Assay Kits, includes purified, active PPARGamma-LBD protein, Fluormone PPARGamma Green ligand kit) or Fluormone PPARGamma Red ligand (red assay kit), and assay buffer. Store components as indicated in the assay protocol.
Info Molecular Probes (Invitrogen)Molecular Probes (Invitrogen)
PO Box 22010
Eugene, OR 97402-0469

Call Molecular Probes (Invitrogen) to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-541-359-2658
Customer Service: 1-541-465-8300
Fax Number: 1-541-344-6504
Web Site:
NOTE: Price information is approximate list price and actual prices may vary.

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