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Phosphodiesterase I from USB Corp.

ProductsPhosphodiesterase I from USB Corp.
Company USB Corp.
Item Phosphodiesterase I
Price 
Description Source: Crotalus adamanteus venom
Description:
Phosphodiesterase I (venom exonuclease) is widely used in studying nucleic acid structure and sequence(1). It hydrolyzes 5'-mononucleotides from 3'-hydroxy-terminated ribo-oligonucleotides and deoxyribo-oligonucleotides(2). Phosphodiesterase I cleaves ADP-ribosylated proteins at the pyrophosphate linkages to yield phosphoribosyl-AMP(3).
Properties:
Molecular Weight: 115,000 Da
Optimum pH: 9.8 - 10.4
Activators: Mg2+
Inhibitors: 5mM EDTA, reducing agents (gluthathione, cysteine and ascorbic acid), partial
inhibition by ATP, ADP and AMP
Form:
Lyophilized powder
Purity:
Partially purified by the method of Bjrk(4) and treated to remove contaminating
5' nucleotidase(5).

Shipping and Storage:
Shipped on dry ice. Store at -20C.
Unit Definition:
One unit hydrolyzes 1M p-nitrophenyl thymidine-5-phosphate per min at 25C, pH 8.9.
Concentration:
greater than or equal to 20 units/mg
Applications:

*Base composition and nearest neighbor analysis(1).
*Removal of cyclic AMP from protein preparations.

References:

*HO, N. W. AND GILHAM, P. T. (1973) Biochem. Biophys. Acta, 308, 53-58.

*LASKOWSKI, M. (1971) in The Enzymes, 3rd Ed. (Academic Press, New York) IV, 313-328.

*HAYAISHI, O. (1976) Trends Biochem. Sci. 1, 9.

*BJRK, W., (1963) J. Biol. Chem. 238, 2487.

*SULKOWSKI, E. AND LASKOWSKI, M. (1971) Biochem. Biophys. Acta, 240, 443.

Info USB Corp.USB Corp.
26111 Miles Road
Cleveland Ohio 44128


Call USB Corp. to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-216-245-1829
Customer Service: 216.765.5000
Fax Number: 216.464.5075
Web Site: http://www.usbweb.com
NOTE: Price information is approximate list price and actual prices may vary.

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