Navigation Links
Phosphodiesterase I from USB Corp.

ProductsPhosphodiesterase I from USB Corp.
Company USB Corp.
Item Phosphodiesterase I
Price 
Description Source: Crotalus adamanteus venom
Description:
Phosphodiesterase I (venom exonuclease) is widely used in studying nucleic acid structure and sequence(1). It hydrolyzes 5'-mononucleotides from 3'-hydroxy-terminated ribo-oligonucleotides and deoxyribo-oligonucleotides(2). Phosphodiesterase I cleaves ADP-ribosylated proteins at the pyrophosphate linkages to yield phosphoribosyl-AMP(3).
Properties:
Molecular Weight: 115,000 Da
Optimum pH: 9.8 - 10.4
Activators: Mg2+
Inhibitors: 5mM EDTA, reducing agents (gluthathione, cysteine and ascorbic acid), partial
inhibition by ATP, ADP and AMP
Form:
Lyophilized powder
Purity:
Partially purified by the method of Bjrk(4) and treated to remove contaminating
5' nucleotidase(5).

Shipping and Storage:
Shipped on dry ice. Store at -20C.
Unit Definition:
One unit hydrolyzes 1M p-nitrophenyl thymidine-5-phosphate per min at 25C, pH 8.9.
Concentration:
greater than or equal to 20 units/mg
Applications:

*Base composition and nearest neighbor analysis(1).
*Removal of cyclic AMP from protein preparations.

References:

*HO, N. W. AND GILHAM, P. T. (1973) Biochem. Biophys. Acta, 308, 53-58.

*LASKOWSKI, M. (1971) in The Enzymes, 3rd Ed. (Academic Press, New York) IV, 313-328.

*HAYAISHI, O. (1976) Trends Biochem. Sci. 1, 9.

*BJRK, W., (1963) J. Biol. Chem. 238, 2487.

*SULKOWSKI, E. AND LASKOWSKI, M. (1971) Biochem. Biophys. Acta, 240, 443.

Info USB Corp.USB Corp.
26111 Miles Road
Cleveland Ohio 44128


Call USB Corp. to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-216-245-1829
Customer Service: 216.765.5000
Fax Number: 216.464.5075
Web Site: http://www.usbweb.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. S-Acetyl Coenzyme A Lithium Salt from USB Corp.
2. Mut S from USB Corp.
3. Thermo Sequenase Dye Primer Manual Cycle Sequencing Kit from USB Corp.
4. Phenol Red, Free Acid from USB Corp.
5. Dithiothreitol from USB Corp.
6. Bromophenol Blue from USB Corp.
7. Xylene Cyanol FF from USB Corp.
8. Ligase, T4 DNA from USB Corp.
9. Human Topoisomerase II, Alpha from USB Corp.
10. Calf Topoisomerase I from USB Corp.
11. M-MLV Reverse Transcriptase from USB Corp.
... The BD Pathway Bioimager is an automated, ... imaging system that has been integrated into ... Pathway was designed to provide high-resolution, automated ... assist in developing better cell-based assays. The ...
... Kit uses a simplified procedure and streamlines ... The kit is based on the three ... and sodium bisulfite where cytosine is converted ... innovative in-column desulphonation reaction eliminates several precipitation ...
... EnzoLyte MG Phosphate Assay Kit is based ... between Malachite Green, molybdate and free orthophosphate. ... can be conveniently measured on a spectrophotometer ... The non-radioactive colorimetric assay kits have been ...
... The carbohydrate analysis standard is ... and a sugar alcohol; molecular weights ... standard is provided for use with ... carbohydrate analysis kit for column testing ...
Biology Products:
(Date:3/20/2017)... , March 20, 2017 PMD Healthcare announces ... spirometer and Wellness Management System (WMS), a remote, real-time ... in 2010, PMD Healthcare is a Medical Device, Digital ... mission dedicated to creating innovative solutions that empower people ... that intent focus, PMD developed the first ever personal ...
(Date:3/13/2017)... of security: Biometric Face Matching software  Continue Reading ... ... match face pictures against each other or against large databases. The recognition of ... ... for biometric Face Matching on the market. The speed is at 100 million ...
(Date:3/9/2017)... FRANCISCO and MOUNTAIN VIEW, Calif. ... , "Eating Well Made Simple," and 23andMe , ... help guide better food choices.  Zipongo can now provide ... their food preferences, health goals and biometrics, but also ... certain food choices. Zipongo,s personalized food decision ...
Breaking Biology News(10 mins):
(Date:3/27/2017)... ... March 27, 2017 , ... IsoPlexis Corporation (IsoPlexis), a ... disease and more through a single-cell precision engineering platform, today announced it has ... in the laboratory of Dr. James Heath at the California Institute of ...
(Date:3/27/2017)... ... March 27, 2017 , ... Biopsies from non-small cell ... with limited tumor content in a large background of normal or wild type ... need for reliable detection of low abundance somatic mutations, particularly in small specimens ...
(Date:3/27/2017)... Md. , March 27, 2017  The ... billion for 2016, according to a new report ... medical lab testing is performed to evaluate disease ... individual therapy, among other reasons.  The healthcare market ... Market , provides an overview of the ...
(Date:3/27/2017)... The new research portal will give visitors quick ... Valero Energy , offering extensive market research on their ... ... The latest trend gaining momentum in ... even though touted as a green alternative to fossil fuels, ...
Breaking Biology Technology: