Navigation Links
Phosphodiesterase I from USB Corp.

ProductsPhosphodiesterase I from USB Corp.
Company USB Corp.
Item Phosphodiesterase I
Price 
Description Source: Crotalus adamanteus venom
Description:
Phosphodiesterase I (venom exonuclease) is widely used in studying nucleic acid structure and sequence(1). It hydrolyzes 5'-mononucleotides from 3'-hydroxy-terminated ribo-oligonucleotides and deoxyribo-oligonucleotides(2). Phosphodiesterase I cleaves ADP-ribosylated proteins at the pyrophosphate linkages to yield phosphoribosyl-AMP(3).
Properties:
Molecular Weight: 115,000 Da
Optimum pH: 9.8 - 10.4
Activators: Mg2+
Inhibitors: 5mM EDTA, reducing agents (gluthathione, cysteine and ascorbic acid), partial
inhibition by ATP, ADP and AMP
Form:
Lyophilized powder
Purity:
Partially purified by the method of Bjrk(4) and treated to remove contaminating
5' nucleotidase(5).

Shipping and Storage:
Shipped on dry ice. Store at -20C.
Unit Definition:
One unit hydrolyzes 1M p-nitrophenyl thymidine-5-phosphate per min at 25C, pH 8.9.
Concentration:
greater than or equal to 20 units/mg
Applications:

*Base composition and nearest neighbor analysis(1).
*Removal of cyclic AMP from protein preparations.

References:

*HO, N. W. AND GILHAM, P. T. (1973) Biochem. Biophys. Acta, 308, 53-58.

*LASKOWSKI, M. (1971) in The Enzymes, 3rd Ed. (Academic Press, New York) IV, 313-328.

*HAYAISHI, O. (1976) Trends Biochem. Sci. 1, 9.

*BJRK, W., (1963) J. Biol. Chem. 238, 2487.

*SULKOWSKI, E. AND LASKOWSKI, M. (1971) Biochem. Biophys. Acta, 240, 443.

Info USB Corp.USB Corp.
26111 Miles Road
Cleveland Ohio 44128


Call USB Corp. to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-216-245-1829
Customer Service: 216.765.5000
Fax Number: 216.464.5075
Web Site: http://www.usbweb.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. S-Acetyl Coenzyme A Lithium Salt from USB Corp.
2. Mut S from USB Corp.
3. Thermo Sequenase Dye Primer Manual Cycle Sequencing Kit from USB Corp.
4. Phenol Red, Free Acid from USB Corp.
5. Dithiothreitol from USB Corp.
6. Bromophenol Blue from USB Corp.
7. Xylene Cyanol FF from USB Corp.
8. Ligase, T4 DNA from USB Corp.
9. Human Topoisomerase II, Alpha from USB Corp.
10. Calf Topoisomerase I from USB Corp.
11. M-MLV Reverse Transcriptase from USB Corp.
D.R. Harper (1993) • Includes: essential information on taxonomy, culture and safe handling of viruses, immunology, monoclonal antibodies, antivirals and vaccines....
...
... PureLink PCR Purification Kits provide rapid and ... short-failed PCR products, and salts from PCR ... procedure:, provides two Binding Buffer options ... , can be performed in either single ...
... The Zero Blunt TOPO ... the fastest and easiest ... than or equal to95%) ... products amplified with proofreading ...
Biology Products:
(Date:2/9/2016)... 9, 2016 Vigilant Solutions announces today that an ... to develop a lead in a difficult homicide case. The ... to locate the suspect vehicle. Due to the ongoing investigation, ... been omitted at the agency,s request. --> ... "Our victim was found deceased at an intersection here in ...
(Date:2/3/2016)... 2016 --> ... "Automated Fingerprint Identification System Market by Component (Hardware and ... & Finance, Government, Healthcare, and Transportation) and Geography - ... is expected to be worth USD 8.49 Billion by ... and 2020. The transformation and technology evolution from the ...
(Date:2/2/2016)... Checkpoint Inhibitors for Cancer – Explore ... you interested in the future of cancer drugs? ... Visiongain,s report gives those predictions to 2026 at ... Avoid falling behind in data or losing ... those emerging cancer therapies can achieve. There you ...
Breaking Biology News(10 mins):
(Date:2/12/2016)... , Feb. 12, 2016  PTC Therapeutics, Inc. ... annual STRIVE (Strategies to Realize Innovation, Vision and ... (DMD). STRIVE provides funds to patient advocacy organizations ... make meaningful contributions to the rare disease community ... of future patient advocates. Mary ...
(Date:2/11/2016)... Non-profit Consortium Aims to Generate Genomic ... Research and Discovery --> --> ... plan to sequence 100,000 individuals. It is intended to initially ... 7 of North and East Asian countries. ... project will focus on creating phased reference genomes for all ...
(Date:2/11/2016)... Texas , Feb. 11, 2016  Vermillion, Inc. ... on gynecologic disease, today announced the formation of the ... --> --> Pelvic masses ... both diagnosis and management. Once pregnancy is ruled out, ... tubes and ovaries, advanced endometriosis, benign ovarian tumors and ...
(Date:2/11/2016)... NEW YORK , Feb. 11, 2016  Bioethics International, ... how medicines are researched, developed, marketed and made accessible to ... BMJ Open had named the publication of the ... for 2015. The publication is also featured as one of ... published in the last year that are most frequently read. ...
Breaking Biology Technology: