|Products||Phosphate Colorimetric Assay Kit, 'EnzoLyte from AnaSpec|
|Item||Phosphate Colorimetric Assay Kit, 'EnzoLyte|
|Description|| EnzoLyte MG Phosphate Assay Kit is based on quantification of the green complex formed between Malachite Green, molybdate and free orthophosphate. The rapid color formation from the reaction can be conveniently measured on a spectrophotometer (600-660 nm) or on a plate reader. The non-radioactive colorimetric assay kits have been optimized to offer superior sensitivity and prolonged shelf life. The assay is simple and fast, involving a single addition step for phosphate determination. Assays can be performed in tubes, cuvettes or multi-well plates. The kit has high sensitivity and wide detection range: detection of as little of 1.6 pmoles of phosphate and useful range between 0.02 mM and 40 mM phosphate. The assays can be conveniently performed in 96- and 384-well plates for high-throughput screening of enzyme inhibitors. The kit has been used for assaying phosphatase, phospholipase or lipase, nucleoside triphosphatase (ATPase, GTPase etc) and phytase. The kit has the following components: Highly purified Malachite Green reagent Assay buffer A mix and read assay protocol that is compatible with HTS liquid handling instruments with Z factors of 0.7 to 0.9.|
Gurpal S, et al. (2003). Characterization of organic phosphorus in leachate from a grassland soil. Soil Biology & Biochemistry 35, 13171323 Cogan EB (1999). A robotics-based automated assay for inorganic and organic phosphates. Anal Biochem 271, 29-35. Fisher DK and Higgins TJ (1994). A sensitive, high-volume, colorimetric assay for protein phosphatases. Pharm Res 11, 759-63.
2149 O Toole Ave.
San Jose, CA 95131
Customer Service: 800-452-5530
Fax Number: 408-452-5059
Web Site: http://www.anaspec.com