Navigation Links
Phosphate Colorimetric Assay Kit, 'EnzoLyte from AnaSpec

ProductsPhosphate Colorimetric Assay Kit, 'EnzoLyte from AnaSpec
Company AnaSpec
Item Phosphate Colorimetric Assay Kit, 'EnzoLyte
Price $195.00
Description EnzoLyte MG Phosphate Assay Kit is based on quantification of the green complex formed between Malachite Green, molybdate and free orthophosphate. The rapid color formation from the reaction can be conveniently measured on a spectrophotometer (600-660 nm) or on a plate reader. The non-radioactive colorimetric assay kits have been optimized to offer superior sensitivity and prolonged shelf life. The assay is simple and fast, involving a single addition step for phosphate determination. Assays can be performed in tubes, cuvettes or multi-well plates. The kit has high sensitivity and wide detection range: detection of as little of 1.6 pmoles of phosphate and useful range between 0.02 mM and 40 mM phosphate. The assays can be conveniently performed in 96- and 384-well plates for high-throughput screening of enzyme inhibitors. The kit has been used for assaying phosphatase, phospholipase or lipase, nucleoside triphosphatase (ATPase, GTPase etc) and phytase. The kit has the following components: Highly purified Malachite Green reagent Assay buffer A mix and read assay protocol that is compatible with HTS liquid handling instruments with Z factors of 0.7 to 0.9.

Gurpal S, et al. (2003). Characterization of organic phosphorus in leachate from a grassland soil. Soil Biology & Biochemistry 35, 13171323 Cogan EB (1999). A robotics-based automated assay for inorganic and organic phosphates. Anal Biochem 271, 29-35. Fisher DK and Higgins TJ (1994). A sensitive, high-volume, colorimetric assay for protein phosphatases. Pharm Res 11, 759-63.

Info AnaSpecAnaSpec
2149 O Toole Ave.
Suite F
San Jose, CA 95131
Customer Service: 800-452-5530
Fax Number: 408-452-5059
Web Site: http://www.anaspec.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Dulbeccos Phosphate Buffered Saline (DPBS) from Sigma-Aldrich
2. Dulbeccos Phosphate Buffered Saline (DPBS) from Sigma-Aldrich
3. Phosphate Sensor from Invitrogen
4. Hexokinase/ Glucose-6-Phosphate Dehydrogenase from Roche Applied Science
5. Hexokinase/ Glucose-6-Phosphate Dehydrogenase from Roche Applied Science
6. Rabbit Anti-Lipid Phosphate Phosphatase 2 (LPP2) Antibody, Unconjugated from Sigma-Aldrich
7. HRP Stabilizing Diluent (Phosphate Buffered) without BSA from ScyTek Laboratories
8. HRP Stabilizing Diluent (Phosphate Buffered) without BSA from ScyTek Laboratories
9. HRP Stabilizing Diluent (Phosphate Buffered) from ScyTek Laboratories
10. HRP Stabilizing Diluent (Phosphate Buffered) from ScyTek Laboratories
11. Calcium Phosphate Transfection Kit from Invitrogen
Calcium Flux Antagonist Chip, 4-Sipper (coated) from Caliper Life Sciences
SYBR Gold nucleic acid gel stain*10,000X concentrate in DMSO* from Molecular Probes (Invitrogen)
Experion RNA StdSens Chips, 10 from Bio-Rad
Experion RNA HighSens Chips, 10 from Bio-Rad
Biology Products:
(Date:11/30/2009)...s a colorless and odorless radioactive gas that is...um is a product of uranium decay and is found in t... and groundwater as well as building materials, pl...entration is expressed as the amount of radiation ...cts in a liter of air; thus the units are picocuri...
(Date:11/26/2009)...r their impressive antlers, red deer have been cau...deer,s antlers are much more than decorative. They... duelling. John Currey, from The University of Yor...ties of bone for over half a century and has becom...r through a long-standing collaboration with Tomas...
(Date:11/26/2009)...carried out in eight European countries has shown ... disease pandemic could have a significant role in...g in the open access journal BMC Infectious Disea...l days and weekends/holidays, finding that they we...rom Hasselt and Antwerp University, Belgium, led a...
Breaking Biology News(10 mins):Health Physics Society recommends considering action for indoor radon below current guidelines 2Tough yet stiff deer antler is materials scientist's dream 2School closure could reduce swine flu transmission by 21 percent 2People Across the Country are Making The Switch 21 53059 1People Across the Country are Making The Switch 21 53059 2Smart for Life 28TM 29 and THINADVENTURE 28TM 29 Sponsor Brandon Flowers Youth Sports Camp 53056 1Smart for Life 28TM 29 and THINADVENTURE 28TM 29 Sponsor Brandon Flowers Youth Sports Camp 53056 2Smart for Life 28TM 29 and THINADVENTURE 28TM 29 Sponsor Brandon Flowers Youth Sports Camp 53056 3ImmuneRegen BioSciences Confirms Homsperas Therapeutic Anti Influenza Effect on Pandemic H1N1 Influenza Virus After Oral Administration 13250 1ImmuneRegen BioSciences Confirms Homsperas Therapeutic Anti Influenza Effect on Pandemic H1N1 Influenza Virus After Oral Administration 13250 2ImmuneRegen BioSciences Confirms Homsperas Therapeutic Anti Influenza Effect on Pandemic H1N1 Influenza Virus After Oral Administration 13250 3
...ed by Rutgers, Samuel Gunderson has developed a no...improvements over existing RNAi approaches. This m...lass of drugs to treat a wide array of diseases. C...m took to specifically target RNA biosynthesis. ,... Nature Biotechnology , published online in the F...
...vance online publication of the journal Nature , ...t viral proteins perform to create the infectious ...ease, say researchers. While the virus they studie... such as herpes and HIV are also known to assemble...its final assault-proof, infectious form. , "The ...
...nerate Energy from Waste Biomass , , ATLANTA, Feb...versified renewable energy company, Global Energy ...ounced the execution of a definitive agreement to ...ndscape materials processor, WoodTech, LLC, and af...expected to occur in mid-February, 2009. , , "We ...
Other Biology News:A new gene silencing platform -- silence is golden 2Scripps scientists create first crystal structure of an intermediate particle in virus assembly 2Scripps scientists create first crystal structure of an intermediate particle in virus assembly 3Global Energy Systems to Acquire WoodTech, LLC, and Affiliated Entities 2Global Energy Systems to Acquire WoodTech, LLC, and Affiliated Entities 3
(Date:11/30/2009)...alif., Nov. 30 BioMarin ...ay that the Food and Drug Administration (FDA) has...ridine (3,4-DAP), amifampridine phosphate, for the...Syndrome (LEMS). 3,4-DAP has previously received ...ber 2009, the Committee for Medicinal Products for...
(Date:11/30/2009)... and COPENHAGEN, Nov. 30 Exiqon A/...hat the Harvard Medical School ICCB-Longwood Scree...croRNA Screening Center of Excellence. ICCB-L sup... associated hospitals and institutions, for high-t...raries. ,, MicroRNAs are a novel class of small...
(Date:11/30/2009)... , AMSTERDAM, November 30 /PRNew...publisher of scientific, medical and technical pro...nch of Embase, its comprehensive biomedical,databa...m more than 7,000,active, peer reviewed journals. ...e advancement of new biomedical,and pharmaceutical...
(Date:11/27/2009)...RHAM, N.C., Nov. 27 Cromoz Inc., l...ter-soluble carbon nanotube-based cancer drug deli... carbon nanotubes, which have functional groups on... drugs, was developed in partnership with the Indi...n nanotubes serves as a drug delivery vehicle with...
Breaking Biology Technology:FDA Grants Orphan Drug Designation for 3,4-DAP for LEMS 2FDA Grants Orphan Drug Designation for 3,4-DAP for LEMS 3FDA Grants Orphan Drug Designation for 3,4-DAP for LEMS 4Exiqon Recognizes Harvard Medical School Core Facility as MicroRNA Screening Center of Excellence 2Exiqon Recognizes Harvard Medical School Core Facility as MicroRNA Screening Center of Excellence 3Elsevier is Re-Launching "Embase" to be a Powerful Resource Providing Deep Insights and Answers to Biomedical Researchers 2Elsevier is Re-Launching "Embase" to be a Powerful Resource Providing Deep Insights and Answers to Biomedical Researchers 3North Carolina Based Cromoz Inc. Launches Carbon Nanotechnology for Target Drug Delivery System in Cancer Treatment in Hyderabad, India 2