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Phagocytosis Fluorometric (dichlorofluorescein) Assay Kit, OxyBURST from Molecular Probes (Invitrogen)

ProductsPhagocytosis Fluorometric (dichlorofluorescein) Assay Kit, OxyBURST from Molecular Probes (Invitrogen)
Company Molecular Probes (Invitrogen)
Item Phagocytosis Fluorometric (dichlorofluorescein) Assay Kit, OxyBURST
Description Molecular Probes Fc OxyBURST Green reagent (F-2902) was developed in collaboration with Elizabeth Simons of Boston University to permit measurement of the kinetics of Fc receptor mediated internalization and the subsequent oxidative burst directly in the phagovacuole.2 The Fc OxyBURST Green assay reagent consists of bovine serum albumin (BSA) that has been covalently linked to dichlorodihydrofluorescein (H2DCF) and then complexed with purified rabbit polyclonal anti-BSA IgG antibodies. When these immune complexes bind to Fc receptors, the nonfluorescent H2DCF molecules are internalized within the phagovacuole and subsequently oxidized to green fluorescent dichlorofluorescein (DCF). Fc OxyBURST Green assay reagent has been found to produce >8 times more fluorescence than does H2DCFDA at 60 seconds and >20 times more at 15 minutes following internalization of the immune complex. Furthermore, unlike H2DCFDA, the Fc OxyBURST Green assay reagent does not require intracellular esterases for activation, making this reagent particularly suitable for detecting the oxidative burst in cells with low esterase activity such as monocytes.
Info Molecular Probes (Invitrogen)Molecular Probes (Invitrogen)
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