Products | PR Competitor Assay Green from Invitrogen | |
Company | Invitrogen | |
Item | PR Competitor Assay Green | |
Price | ||
Description | Progesterone Receptor (PR) Competitor Assay Kits are ideal for studying or screening novel progesterone receptor binding compounds using fluorescence polarization (FP) The homogenous assay utilizes a fusion of glutathione transferase to the ligand binding domain of human progesterone receptor [PR-LBD(GST)] and a proprietary, fluorescently-tagged progesterone ligand, Fluormone PL Green or PL Red. Intrinsic background fluorescence from library compounds is substantially reduced utilizing the red-shifted Fluormone ligand. To assay: PR added to Fluormone L Green or PL Red complexes and has a high polarization value. Test compounds that compete for binding to PR and prevent the formation of a Fluormone/PR complex will result in a lower polarization value. The shift in polarization in the presence of test compounds is used to determine the relative affinity of test compounds for PR. Contents: The Progesterone Receptor Competitor Assay Kits contain partially purified PR protein, Fluormone PL Green (green assay kit) or PL Red (red assay kit) proprietary fluorescent progesterone ligands, and buffer. | |
Info | ![]() Invitrogen Corporation 1600 Faraday Ave. Carlsbad, CA 92008
Tech Support: 800-955-6288 ext. 2 Fax Number: 760-603-7229 Web Site: http://www.invitrogen.com |