Navigation Links
PPARGamma-Competitor Assay Kit Green from Invitrogen

ProductsPPARGamma-Competitor Assay Kit Green from Invitrogen
Company Invitrogen
Item PPARGamma-Competitor Assay Kit Green
Price 
Description Peroxisome Proliferator-Activated Receptor-gamma (PPAR) is a member of a nuclear receptor family of ligand-activated transcription factors that heterodimerize with retinoic acid-like receptor (RXR). It is involved in the regulation of glucose and lipid metabolism. Agonists include the (TZD) class of anti-diabetic agents called thiazolidinediones (TZD) or "glitazones." Rosiglitazone (Avandia) and Pioglitazone (Actos) are two PPAR agonists currently marketed for treatment of type 2 diabetes. PPAR agonists may also be therapeutically important in the treatment of coronary artery disease, obesity, and cancer.


The Peroxisome Proliferator-Activated Receptor-gamma (PPAR) Competitor Assay Kit, Green provides a sensitive and efficient method for studying and screening potential PPAR ligands using fluorescence polarization (FP). The kit contains human PPAR 1-ligand binding domain (PPAR-LBD) with an N-terminal His-tag and a novel, selective fluorescent PPAR ligand (Fluormone PPAR Green) in a homogenous mix-and-read assay format.


Assay mechanism:
PPAR-LBD is added to Fluormone PPAR Green to form a PPAR-LBD/Fluormone PPAR Green complex. Binding of the PPAR-LBD to the Fluormone PPAR Green ligand results in a high polarization value. This complex is then added to individual test compounds in microwell plates. Competitors displace the Fluormone PPAR Green ligand from the PPAR-LBD/Fluormone PPAR Green complex, causing the Fluormone PPAR Green ligand to tumble rapidly during its fluorescent lifetime and resulting in a low polarization value. Non-competitors will not displace the Fluormone PPAR Green ligand from the complex, so the polarization value remains high. The shift in polarization value in the presence of test compounds is used to determine the relative affinity of test compounds for PPAR-LBD.


Contents:
The PPAR-Competitor Assay Kit, Green includes purified, active PPAR-LBD protein, Fluormone PPAR Green ligand, and assay buffer
Info InvitrogenInvitrogen
Invitrogen Corporation
1600 Faraday Ave.
Carlsbad, CA 92008

Call Invitrogen to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-866-521-4270
Customer Service: 800-955-6288
Tech Support: 800-955-6288 ext. 2
Fax Number: 760-603-7229
Web Site: http://www.invitrogen.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. PolarScreen PPARGamma-Competitor Assay Kit, Red from Molecular Probes (Invitrogen)
2. Glutathione-S-Transferase (GST) Colorimetric Assay Kit from Sigma-Aldrich
3. In Vitro Osteogenesis Assay Kit from CHEMICON
4. Mast Cell Degranulation colorimetric Assay Kit from CHEMICON
5. PR Competitor Assay Green from Invitrogen
6. PolarScreen Androgen Receptor Competitor Assay Kit, Red from Molecular Probes (Invitrogen)
7. Hydrogen Peroxide / Peroxidase Fluorometric (resorufin) Assay Kit, Fluoro H2O2 from BACHEM
8. Total antioxidant capacity Colorimetric Assay Kit from Neogen Corporation
9. PolarScreen Progesterone Receptor Competitor Assay Kit, Far Red from Molecular Probes (Invitrogen)
10. Total Antioxidant Power Colorimetric Assay Kit from Neogen Corporation
11. NDPase (GDPase, UDPase, ADPase) Assay Kit from Sigma-Aldrich
(Date:4/14/2016)... , April 14, 2016 ... and Malware Detection, today announced the appointment of ... the new role. Goldwerger,s leadership appointment comes ... the heels of the deployment of its platform at ... behavioral biometric technology, which discerns unique cognitive and physiological ...
(Date:3/31/2016)... , March 31, 2016 ... ) ("LegacyXChange" or the "Company") LegacyXChange is ... users of its soon to be launched online site ... https://www.youtube.com/channel/UCyTLBzmZogV1y2D6bDkBX5g ) will also provide potential shareholders ... of DNA technology to an industry that is notorious ...
(Date:3/29/2016)... Florida , March 29, 2016 ... the "Company") LegacyXChange "LEGX" and SelectaDNA/CSI Protect are pleased ... in ink used in a variety of writing instruments, ... Buyers of originally created collectibles from athletes on LegacyXChange ... forensic analysis of the DNA. Bill ...
Breaking Biology News(10 mins):
(Date:5/2/2016)... 2, 2016 Q BioMed Inc. ... partner Mannin Research Inc. will be attending the Association ... place from May 1-5, 2016 in Seattle ... with its vendors and research partners. The meeting provides ... and other collaborative opportunities for the MAN-01 program for ...
(Date:4/29/2016)... , ... April 29, 2016 , ... ... personalized pain medicine, is excited to announce the launch of the Proove ... health studies, volunteerism, and education to promote the use of personalized medicine for ...
(Date:4/29/2016)... (PRWEB) , ... April 29, 2016 , ... Intelligent Implant ... by the FDA via 510(k) for sale in the United States. These components ... posterior thoraco-lumbar fusions. With one-level sales beginning in October of 2015, the company ...
(Date:4/28/2016)... ... 2016 , ... Next week on May 5 at 2:55 ... for tissue stem cell counting and expansion to gene-editing scientists and other attendees ... Genome Engineering in Burlington, Massachusetts. , The attention of most gene-editing scientists appears ...
Breaking Biology Technology: