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PPARGamma-Competitor Assay Kit Green from Invitrogen

ProductsPPARGamma-Competitor Assay Kit Green from Invitrogen
Company Invitrogen
Item PPARGamma-Competitor Assay Kit Green
Description Peroxisome Proliferator-Activated Receptor-gamma (PPAR) is a member of a nuclear receptor family of ligand-activated transcription factors that heterodimerize with retinoic acid-like receptor (RXR). It is involved in the regulation of glucose and lipid metabolism. Agonists include the (TZD) class of anti-diabetic agents called thiazolidinediones (TZD) or "glitazones." Rosiglitazone (Avandia) and Pioglitazone (Actos) are two PPAR agonists currently marketed for treatment of type 2 diabetes. PPAR agonists may also be therapeutically important in the treatment of coronary artery disease, obesity, and cancer.

The Peroxisome Proliferator-Activated Receptor-gamma (PPAR) Competitor Assay Kit, Green provides a sensitive and efficient method for studying and screening potential PPAR ligands using fluorescence polarization (FP). The kit contains human PPAR 1-ligand binding domain (PPAR-LBD) with an N-terminal His-tag and a novel, selective fluorescent PPAR ligand (Fluormone PPAR Green) in a homogenous mix-and-read assay format.

Assay mechanism:
PPAR-LBD is added to Fluormone PPAR Green to form a PPAR-LBD/Fluormone PPAR Green complex. Binding of the PPAR-LBD to the Fluormone PPAR Green ligand results in a high polarization value. This complex is then added to individual test compounds in microwell plates. Competitors displace the Fluormone PPAR Green ligand from the PPAR-LBD/Fluormone PPAR Green complex, causing the Fluormone PPAR Green ligand to tumble rapidly during its fluorescent lifetime and resulting in a low polarization value. Non-competitors will not displace the Fluormone PPAR Green ligand from the complex, so the polarization value remains high. The shift in polarization value in the presence of test compounds is used to determine the relative affinity of test compounds for PPAR-LBD.

The PPAR-Competitor Assay Kit, Green includes purified, active PPAR-LBD protein, Fluormone PPAR Green ligand, and assay buffer
Info InvitrogenInvitrogen
Invitrogen Corporation
1600 Faraday Ave.
Carlsbad, CA 92008

Call Invitrogen to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-866-521-4270
Customer Service: 800-955-6288
Tech Support: 800-955-6288 ext. 2
Fax Number: 760-603-7229
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NOTE: Price information is approximate list price and actual prices may vary.

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