Navigation Links
PPARGamma-Competitor Assay Kit Green from Invitrogen

ProductsPPARGamma-Competitor Assay Kit Green from Invitrogen
Company Invitrogen
Item PPARGamma-Competitor Assay Kit Green
Price 
Description Peroxisome Proliferator-Activated Receptor-gamma (PPAR) is a member of a nuclear receptor family of ligand-activated transcription factors that heterodimerize with retinoic acid-like receptor (RXR). It is involved in the regulation of glucose and lipid metabolism. Agonists include the (TZD) class of anti-diabetic agents called thiazolidinediones (TZD) or "glitazones." Rosiglitazone (Avandia) and Pioglitazone (Actos) are two PPAR agonists currently marketed for treatment of type 2 diabetes. PPAR agonists may also be therapeutically important in the treatment of coronary artery disease, obesity, and cancer.


The Peroxisome Proliferator-Activated Receptor-gamma (PPAR) Competitor Assay Kit, Green provides a sensitive and efficient method for studying and screening potential PPAR ligands using fluorescence polarization (FP). The kit contains human PPAR 1-ligand binding domain (PPAR-LBD) with an N-terminal His-tag and a novel, selective fluorescent PPAR ligand (Fluormone PPAR Green) in a homogenous mix-and-read assay format.


Assay mechanism:
PPAR-LBD is added to Fluormone PPAR Green to form a PPAR-LBD/Fluormone PPAR Green complex. Binding of the PPAR-LBD to the Fluormone PPAR Green ligand results in a high polarization value. This complex is then added to individual test compounds in microwell plates. Competitors displace the Fluormone PPAR Green ligand from the PPAR-LBD/Fluormone PPAR Green complex, causing the Fluormone PPAR Green ligand to tumble rapidly during its fluorescent lifetime and resulting in a low polarization value. Non-competitors will not displace the Fluormone PPAR Green ligand from the complex, so the polarization value remains high. The shift in polarization value in the presence of test compounds is used to determine the relative affinity of test compounds for PPAR-LBD.


Contents:
The PPAR-Competitor Assay Kit, Green includes purified, active PPAR-LBD protein, Fluormone PPAR Green ligand, and assay buffer
Info InvitrogenInvitrogen
Invitrogen Corporation
1600 Faraday Ave.
Carlsbad, CA 92008

Call Invitrogen to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-866-521-4270
Customer Service: 800-955-6288
Tech Support: 800-955-6288 ext. 2
Fax Number: 760-603-7229
Web Site: http://www.invitrogen.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. PolarScreen PPARGamma-Competitor Assay Kit, Red from Molecular Probes (Invitrogen)
2. Glutathione-S-Transferase (GST) Colorimetric Assay Kit from Sigma-Aldrich
3. In Vitro Osteogenesis Assay Kit from CHEMICON
4. Mast Cell Degranulation colorimetric Assay Kit from CHEMICON
5. PR Competitor Assay Green from Invitrogen
6. PolarScreen Androgen Receptor Competitor Assay Kit, Red from Molecular Probes (Invitrogen)
7. Hydrogen Peroxide / Peroxidase Fluorometric (resorufin) Assay Kit, Fluoro H2O2 from BACHEM
8. Total antioxidant capacity Colorimetric Assay Kit from Neogen Corporation
9. PolarScreen Progesterone Receptor Competitor Assay Kit, Far Red from Molecular Probes (Invitrogen)
10. Total Antioxidant Power Colorimetric Assay Kit from Neogen Corporation
11. NDPase (GDPase, UDPase, ADPase) Assay Kit from Sigma-Aldrich
... Design with Purpose -- The ... provide the highest level of ... in an affordable, easy-to-use system. ... and workstation guarantees success with ...
Homo sapiens 4-aminobutyrate aminotransferase Antigen: Recombinant Protein...
Human DCIR/CLEC4A Phycoerythrin MAb (Clone 216110)...
Rubeola (Measles) Antigen Preservative: No Preservatives...
Biology Products:
(Date:6/13/2019)... BOSTON (PRWEB) , ... June 13, 2019 , ... ... Future of Spine & The Spine, Orthopedic and Pain Management-Driven ASC Conference in Chicago ... Hotels & Resorts 323 E. Wacker Dr. Chicago, IL in the 3rd floor Ticino ...
(Date:6/11/2019)... ... June 12, 2019 , ... With a rapidly growing ... leverage the Geneious Biologics platform as a premium hub for bioinformatics and computer ... says: “The Geneious team has done an excellent job accommodating our specific data, ...
(Date:6/6/2019)... ... June 05, 2019 , ... MedTech Breakthrough , ... health and medical technology market, announced that it has selected Medacta International’s MySpine® ... award in the 2019 MedTech Breakthrough Awards program. , Medacta® ...
Breaking Biology News(10 mins):
(Date:5/31/2019)... (PRWEB) , ... May 29, 2019 , ... For many ... the toughest forms of cancer demand breakthrough therapies. Advances in immuno-oncology have led to ... patient’s own T cells with engineered T cell receptors known as “CARs”. The CAR ...
(Date:5/31/2019)... ... May 30, 2019 , ... Object Pharma, Inc., a startup ... of Metabiologics, Inc., a globally recognized leader in the production and sale of ... to a full spectrum of botulinum neurotoxins, as well as a CDC approved ...
(Date:5/21/2019)... Ethiopia (PRWEB) , ... May 21, 2019 , ... ... Brauerei in Berlin (VLB) today officially opened the inaugural Africa Brewing Conference dedicated ... and malting industry in Africa. The event, which is supported through a partnership ...
(Date:5/14/2019)... ... May 13, 2019 , ... Leak Detection Associates (LDA), ... Pharmaceutical, Biotechnology, Medical Device and Food Packaging Industries’ is excited to announce the ... improve LDA’s ability to communicate directly with the marketplace; serving as a destination ...
Breaking Biology Technology: