Navigation Links
PPARGamma-Competitor Assay Kit Green from Invitrogen

ProductsPPARGamma-Competitor Assay Kit Green from Invitrogen
Company Invitrogen
Item PPARGamma-Competitor Assay Kit Green
Price 
Description Peroxisome Proliferator-Activated Receptor-gamma (PPAR) is a member of a nuclear receptor family of ligand-activated transcription factors that heterodimerize with retinoic acid-like receptor (RXR). It is involved in the regulation of glucose and lipid metabolism. Agonists include the (TZD) class of anti-diabetic agents called thiazolidinediones (TZD) or "glitazones." Rosiglitazone (Avandia) and Pioglitazone (Actos) are two PPAR agonists currently marketed for treatment of type 2 diabetes. PPAR agonists may also be therapeutically important in the treatment of coronary artery disease, obesity, and cancer.


The Peroxisome Proliferator-Activated Receptor-gamma (PPAR) Competitor Assay Kit, Green provides a sensitive and efficient method for studying and screening potential PPAR ligands using fluorescence polarization (FP). The kit contains human PPAR 1-ligand binding domain (PPAR-LBD) with an N-terminal His-tag and a novel, selective fluorescent PPAR ligand (Fluormone PPAR Green) in a homogenous mix-and-read assay format.


Assay mechanism:
PPAR-LBD is added to Fluormone PPAR Green to form a PPAR-LBD/Fluormone PPAR Green complex. Binding of the PPAR-LBD to the Fluormone PPAR Green ligand results in a high polarization value. This complex is then added to individual test compounds in microwell plates. Competitors displace the Fluormone PPAR Green ligand from the PPAR-LBD/Fluormone PPAR Green complex, causing the Fluormone PPAR Green ligand to tumble rapidly during its fluorescent lifetime and resulting in a low polarization value. Non-competitors will not displace the Fluormone PPAR Green ligand from the complex, so the polarization value remains high. The shift in polarization value in the presence of test compounds is used to determine the relative affinity of test compounds for PPAR-LBD.


Contents:
The PPAR-Competitor Assay Kit, Green includes purified, active PPAR-LBD protein, Fluormone PPAR Green ligand, and assay buffer
Info InvitrogenInvitrogen
Invitrogen Corporation
1600 Faraday Ave.
Carlsbad, CA 92008

Call Invitrogen to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-866-521-4270
Customer Service: 800-955-6288
Tech Support: 800-955-6288 ext. 2
Fax Number: 760-603-7229
Web Site: http://www.invitrogen.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. PolarScreen PPARGamma-Competitor Assay Kit, Red from Molecular Probes (Invitrogen)
2. Glutathione-S-Transferase (GST) Colorimetric Assay Kit from Sigma-Aldrich
3. In Vitro Osteogenesis Assay Kit from CHEMICON
4. Mast Cell Degranulation colorimetric Assay Kit from CHEMICON
5. PR Competitor Assay Green from Invitrogen
6. PolarScreen Androgen Receptor Competitor Assay Kit, Red from Molecular Probes (Invitrogen)
7. Hydrogen Peroxide / Peroxidase Fluorometric (resorufin) Assay Kit, Fluoro H2O2 from BACHEM
8. Total antioxidant capacity Colorimetric Assay Kit from Neogen Corporation
9. PolarScreen Progesterone Receptor Competitor Assay Kit, Far Red from Molecular Probes (Invitrogen)
10. Total Antioxidant Power Colorimetric Assay Kit from Neogen Corporation
11. NDPase (GDPase, UDPase, ADPase) Assay Kit from Sigma-Aldrich
... The 10X Automation Buffer is a concentrated ... in autostainers employing capillary action technology, as ... developed in collaboration with David Brigati M.D., ... City, OK. This buffer is recommended for ...
... O-TOF creates a new category for MALDI. ... that has been licensed to the PerkinElmerSCIEX ... , As the first single MALDI-TOF mass ... cooling, the prOTOF 2000 represents a substantial ...
... ideal small concentrator for the molecular biology ... water and organic solvents from a variety ... and round bottom flasks. Making it an ... ,The built-in special runs for working with ...
... H2OBIT™ is a fully licensed, high throughput, water ... of up to 24 microplates. This equates to ... A robotic arm rapidly transfers a ... in temperature ramping times that are considerably faster ...
Biology Products:
(Date:1/20/2019)... Mass. (PRWEB) , ... January 18, 2019 , ... Boston-based ... SmartLabs in an effort to better express its continued expansion and service ... innovation calls for a new kind of lab: one that is more agile and ...
(Date:1/14/2019)... ... January 14, 2019 , ... ... EST (4pm GMT) to hear about key topics in data quality, including:, ... ratings ,     Patient engagement ,     Smartphone and wearable based performance measures ...
(Date:1/10/2019)... ... January 10, 2019 , ... When Dr. James L. ... , was invited to contribute a chapter to the new book Perinatal Stem Cells: ... continue Asymmetrex’s effort to address a debilitating secret of stem cell medicine. For ...
Breaking Biology News(10 mins):
(Date:1/15/2019)... ... 15, 2019 , ... With the U.S. Congress designating January as Cervical Health Awareness Month ... today announces tips to help reduce the risk of Cervical Cancer. , Cervical cancer ... is the #1 most common STD and an STI that almost half of ...
(Date:1/10/2019)... ... January 08, 2019 , ... ... an innovative spinal implant company, announced today that SAGICO will be represented ... The annual J.P. Morgan Healthcare Conference is the largest and most ...
(Date:1/7/2019)... SEONGNAM, South Korea (PRWEB) , ... January 07, ... ... has completed Phase 1 clinical trials of its Parkinson's Disease drug candidate, code-named ... disease-modifying treatment of Parkinson's Disease. The trial took place in South Korea. ...
(Date:1/4/2019)... ... January 03, 2019 , ... Calidi Biotherapeutics, ... for cancer, today announced the founding of a scientific and medical advisory board ... from key leaders in the Immuno-Oncology field. The founding members of Calidi Biotherapeutics ...
Breaking Biology Technology: