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Origami(DE3)pLysS Glycerol Stock from Novagen

ProductsOrigami(DE3)pLysS Glycerol Stock from Novagen
Company Novagen
Item Origami(DE3)pLysS Glycerol Stock
Price $84.00
Description Origami host strains are K-12 derivatives that have mutations in both the thioredoxin reductase (trxB) and glutathione reductase (gor) genes, which greatly enhances disulfide bond formation in the cytoplasm. Studies have shown that expression in Origami(DE3) yielded 10-fold more active protein than in another host even though overall expression levels were similar (1). Origami hosts are compatible with ampicillin-resistant plasmids and are ideal for use with pET-32 vectors, since the thioredoxin fusion tag further enhances the formation of disulfide bonds in the cytoplasm. The Origami(DE3)pLacI host is compatible with expression from pETBlue and pTriEx vectors. The trxB and gor mutations are selectable on kanamycin and tetracycline, respectively; therefore, this strain is recommended for use with pET plasmids carrying the ampicillin resistance marker bla.
Info NovagenNovagen
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