| Products | Nucleosome ELISA Kit from Calbiochem |
| Company | Calbiochem |
| Item | Nucleosome ELISA Kit |
| Price | $282.00 |
| Description | PROTECT FROM MOISTURE. AVOID FREEZE/THAW CYCLES. This kit allows the quantitation of apoptotic cells in vitro by DNA affinity-mediated capture of free nucleosomes followed by their anti-histone-facilitated detection. In this assay, mono- and oligonucleosomes are captured on pre-coated DNA binding proteins. Anti-Histone 3 (H3) biotin-labeled antibody then binds to the histone component of captured nucleosomes and is detected following incubation with streptavidin-HRP conjugate. HRP catalyzes the conversion of colorless tetramethylbenzidine to blue. The addition of stop solution changes the color to yellow, the intensity of which is proportional to the number of nucleosomes in the sample. By comparing the absorbance of unknown with that obtained from the standards, one can assign a nucleosome unit value to the unknown sample. Can be used to assign apoptotic index. One unit is defined as the amount of nucleosomes from 444 UV-treated Daudi cells. Sensitivity: 0.15 units/ml |
| Info | CalbiochemCalbiochem, A Brand of EMD Biosciences, Inc. 10394 Pacific Center Court San Diego, CA 92121 USA Customer Service: (800) 854-3417 (858) 450 9600 Tech Support: 800-628-8470 Fax Number: (800) 776-0999 (858) 453 3552 Web Site: http://www.calbiochem.com |