Navigation Links
Neural Stem Cell Expansion Kit - Neurosphere from R&D Systems

ProductsNeural Stem Cell Expansion Kit - Neurosphere from R&D Systems
Company R&D Systems
Item Neural Stem Cell Expansion Kit - Neurosphere
Price $300.00
Description The Neural Stem Cell Expansion Kit - Neurosphere is a system designed for in vitro neural stem cell expansion as neurospheres in a serum free environment. The kit contains a specially formulated N-2 Plus Media Supplement, which has been optimized for neural stem cell expansion (Johe, K.K. et al. (1996) Genes & Development 10:3129). Two growth factors, human epidermal growth factor (EGF) and human fibroblast growth factor basic (FGF basic), are included to promote the in vitroproliferation of neural precursors. The quantity of each component provided in the kit is estimated to be sufficient to make 500 mL of growth medium for cell expansion. This kit is suitable for the neurosphere culture model and is applicable for human, rat and mouse cells. Cells propagated using this kit are Nestin-positive (Lendahl, U. et al. (1990) Cell 60:585) and retain their potency to differentiate into neurons, oligodendrocytes and glia, as identified after growth factor withdrawal by staining with the antibodies Tuj1, O4 and anti-GFAP, respectively.

Neural stem cells (NSCs), with the potential to self-renew and to differentiate into neurons and glial cells, have been isolated from different regions of the developing and adult brain (1 - 6). NSCs can be grown in vitro, in the presence of mitogen, as either monolayer cultures on an adhesive substrate or as free floating spherical aggregates termed 'neurospheres. These in vitro propagated NSCs can proliferate in culture while retaining the potency to differentiate into neurons, oligodendrocytes and glia. in vitro NSC expansion makes it possible to grow sufficient numbers of NSCs for researchers to study basic developmental biology, drug discovery and preclinical neural transplantation.

  1. Alvarez-Buylla, A. et al. (1998) J. Neurobiol. 36:105.
  2. McKay, R.D. (1997) Science 276:66.
  3. Reynolds, B.A. et al. (1992) Science 255:1707.
  4. Johansson, C.B. et al. (1999) Cell 96:25.
  5. Kilpatrick, T.J. et al. (1993) Neuron 10:255.
  6. Davis, A.A. et al. (1994) Nature 372:263.

Neural Stem Cell Expansion Kits SC002 & SC005 are suitable for a monolayer culture model, and Neural Stem Cell Expansion Kit SC003 is for a neurosphere culture model.

Info R&D SystemsR&D Systems
R&D Systems, Inc.
614 McKinley Place N.E.
Minneapolis, MN 55413
Customer Service: 1 (800) 343-7475
1 (612) 379-2956

Fax Number: 1 (612) 656-4400
Web Site: http://www.rndsystems.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. NeuroCyte - Rat Neural Stem Cell Kit from Orion BioSolutions, Inc.
2. Neural Stem Cell Expansion Kit - Monolayer Plus from R&D Systems
3. Neural Stem Cell Expansion Kit - Monolayer from R&D Systems
4. Mouse/Rat Neural Stem Cell Functional Identification Kit from R&D Systems
5. Human Neural Stem Cell Functional Identification Kit from R&D Systems
6. rNeural Stem Cell (rNSC)Kit from AlphaGenix, Inc.
7. Expansion and Cryopreservation of Cells from Meridian Life Science, Inc.
8. Stem Cell Expansion Media 1 from 3H Biomedical AB
9. Mouse Hematopoietic Stem Cell Expansion Cytokine Panel from R&D Systems
10. Mouse Dopaminergic Neuron Differentiation Kit, 2nd Generation from R&D Systems
11. Free Activin A ELISA Kit from Diagnostic Systems Laboratories, Inc.
... BCIP/NBT Chromogen system consists of ... in a single component,solution. The ... the presence of alkaline phosphatase ... with minimal background noise. ,The ...
... The Infinite 200 series of microplate ... or absorbance detection in an affordable, easy-to-use ... science applications. The unique modular design and ... researchers with a choice of a monochromator ...
Mouse monoclonal antibody raised against a partial recombinant KRT4. NCBI Entrez Gene ID = KRT4...
Mouse monoclonal antibody raised against a partial recombinant MYO7A. NCBI Entrez Gene ID = MYO7A...
Biology Products:
(Date:2/10/2017)... Research and Markets has announced the addition of ... Commercial Aspects" to their offering. ... Biomarkers play an important ... selection of treatment as well for monitoring the results. There ... modern medicine. Biochip/microarray technologies and next generation sequencing are also ...
(Date:2/8/2017)... Inc. (NASDAQ: AWRE ), a leading supplier of ... quarter and year ended December 31, 2016. ... compared to $6.9 million in the same quarter last year. ... million compared to $2.6 million in the fourth quarter of ... $0.5 million, or $0.02 per diluted share, which compares to ...
(Date:2/8/2017)... 7, 2017 Report Highlights The ... from $8.3 billion in 2016 at a compound annual ... Report Includes - An overview of the global ... with data from 2015 and 2016, and projections of ... of the market on the basis of product type, ...
Breaking Biology News(10 mins):
(Date:2/22/2017)... Scientists propose in Nature blocking ... Gaucher and maybe other lysosomal storage diseases as a ... current therapies. An international research team led ... also included investigators from the University of Lübeck in ... 22. The study was conducted in mouse models of ...
(Date:2/22/2017)... Yorba Linda, Ca (PRWEB) , ... February 22, ... ... interactive virtual events for tech innovators, engineers, and scientists from around the world, ... event will place on February 22 and 23, 2017. This premier, online-only conference ...
(Date:2/22/2017)... , Feb. 22, 2017 Origin (Origin Agritech, LLC, a ... and seed provider, and Arcadia (Arcadia Biosciences, ... develops and commercializes agricultural productivity traits and nutritional products, today announced ... biotechnology product developed in China to ... trials. ...
(Date:2/22/2017)... ... February 22, 2017 , ... Pharma and biotech consulting ... European director. Operating from Pennside’s Zurich headquarters, Pennside Partners, GmbH, Mr. Perkins brings ... after more than a decade with leading market research firm, GfK. He began ...
Breaking Biology Technology: