Navigation Links
Neural Stem Cell Expansion Kit - Neurosphere from R&D Systems

ProductsNeural Stem Cell Expansion Kit - Neurosphere from R&D Systems
Company R&D Systems
Item Neural Stem Cell Expansion Kit - Neurosphere
Price $300.00
Description The Neural Stem Cell Expansion Kit - Neurosphere is a system designed for in vitro neural stem cell expansion as neurospheres in a serum free environment. The kit contains a specially formulated N-2 Plus Media Supplement, which has been optimized for neural stem cell expansion (Johe, K.K. et al. (1996) Genes & Development 10:3129). Two growth factors, human epidermal growth factor (EGF) and human fibroblast growth factor basic (FGF basic), are included to promote the in vitroproliferation of neural precursors. The quantity of each component provided in the kit is estimated to be sufficient to make 500 mL of growth medium for cell expansion. This kit is suitable for the neurosphere culture model and is applicable for human, rat and mouse cells. Cells propagated using this kit are Nestin-positive (Lendahl, U. et al. (1990) Cell 60:585) and retain their potency to differentiate into neurons, oligodendrocytes and glia, as identified after growth factor withdrawal by staining with the antibodies Tuj1, O4 and anti-GFAP, respectively.

Neural stem cells (NSCs), with the potential to self-renew and to differentiate into neurons and glial cells, have been isolated from different regions of the developing and adult brain (1 - 6). NSCs can be grown in vitro, in the presence of mitogen, as either monolayer cultures on an adhesive substrate or as free floating spherical aggregates termed 'neurospheres. These in vitro propagated NSCs can proliferate in culture while retaining the potency to differentiate into neurons, oligodendrocytes and glia. in vitro NSC expansion makes it possible to grow sufficient numbers of NSCs for researchers to study basic developmental biology, drug discovery and preclinical neural transplantation.

  1. Alvarez-Buylla, A. et al. (1998) J. Neurobiol. 36:105.
  2. McKay, R.D. (1997) Science 276:66.
  3. Reynolds, B.A. et al. (1992) Science 255:1707.
  4. Johansson, C.B. et al. (1999) Cell 96:25.
  5. Kilpatrick, T.J. et al. (1993) Neuron 10:255.
  6. Davis, A.A. et al. (1994) Nature 372:263.

Neural Stem Cell Expansion Kits SC002 & SC005 are suitable for a monolayer culture model, and Neural Stem Cell Expansion Kit SC003 is for a neurosphere culture model.

Info R&D SystemsR&D Systems
R&D Systems, Inc.
614 McKinley Place N.E.
Minneapolis, MN 55413
Customer Service: 1 (800) 343-7475
1 (612) 379-2956

Fax Number: 1 (612) 656-4400
Web Site: http://www.rndsystems.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. NeuroCyte - Rat Neural Stem Cell Kit from Orion BioSolutions, Inc.
2. Neural Stem Cell Expansion Kit - Monolayer Plus from R&D Systems
3. Neural Stem Cell Expansion Kit - Monolayer from R&D Systems
4. Mouse/Rat Neural Stem Cell Functional Identification Kit from R&D Systems
5. Human Neural Stem Cell Functional Identification Kit from R&D Systems
6. rNeural Stem Cell (rNSC)Kit from AlphaGenix, Inc.
7. Expansion and Cryopreservation of Cells from Meridian Life Science, Inc.
8. Stem Cell Expansion Media 1 from 3H Biomedical AB
9. Mouse Hematopoietic Stem Cell Expansion Cytokine Panel from R&D Systems
10. Mouse Dopaminergic Neuron Differentiation Kit, 2nd Generation from R&D Systems
11. Free Activin A ELISA Kit from Diagnostic Systems Laboratories, Inc.
... Rack inserts fit into the ... allow you to greatly increase ... deepwell format plates at an ... from the shaker at once ...
For quantitative determination of Aldolase in Serum (an indicator of muscle damage). Ready-to-use liquid stable reagent. Formulated to eliminate interference from pyruvates. Application sheets are ...
Request Info...
... X-Gal ,MOLECULAR BIOLOGY GRADE ,5-Bromo-4-chloro-3-indoxyl-beta-D-galactopyranoside C ... Cl N O 6 ,M.W.= ... ,Solution: Clear (2% in DMF) ,Biological ... and M13 ,Elemental Analysis: Agrees with ...
Biology Products:
(Date:4/19/2017)... 19, 2017 The global military ... is marked by the presence of several large global ... by five major players - 3M Cogent, NEC Corporation, ... for nearly 61% of the global military biometric market ... the global military biometrics market boast global presence, which ...
(Date:4/13/2017)... According to a new market research report "Consumer IAM Market ... Authorization), Service, Authentication Type, Deployment Mode, Vertical, and Region - Global Forecast ... from USD 14.30 Billion in 2017 to USD 31.75 Billion by 2022, ... ... MarketsandMarkets Logo ...
(Date:4/11/2017)... , April 11, 2017 NXT-ID, ... security technology company, announces the appointment of independent Directors Mr. ... to its Board of Directors, furthering the company,s corporate ... ... NXT-ID, we look forward to their guidance and benefiting from ...
Breaking Biology News(10 mins):
(Date:10/10/2017)... ... 10, 2017 , ... The Pittcon Program Committee is pleased ... scientists who have made outstanding contributions to analytical chemistry and applied spectroscopy. Each ... leading conference and exposition for laboratory science, which will be held February 26-March ...
(Date:10/9/2017)... N.C. (PRWEB) , ... October 09, 2017 , ... At ... announced Dr. Christopher Stubbs, a professor in Harvard University’s Departments of Physics and Astronomy, ... Stubbs was a member of the winning team for the 2015 Breakthrough Prize in ...
(Date:10/7/2017)... , ... October 06, 2017 ... ... experience providing advanced instruments and applications consulting for microscopy and surface analysis, ... in application consulting, Nanoscience Analytical offers a broad range of contract analysis ...
(Date:10/6/2017)... ... 06, 2017 , ... The HealthTech Venture Network (HTVN) is ... fourth annual Conference where founders, investors, innovative practitioners and collaborators are invited to ... showcasing early stage digital health and med tech companies. , This day-long event ...
Breaking Biology Technology: