Navigation Links
Neural Stem Cell Expansion Kit - Monolayer Plus from R&D Systems

ProductsNeural Stem Cell Expansion Kit - Monolayer Plus from R&D Systems
Company R&D Systems
Item Neural Stem Cell Expansion Kit - Monolayer Plus
Price $335.00
Description The Neural Stem Cell Expansion Kit - Monolayer Plus is a system designed for in vitro NSC expansion as a monolayer in a serum-free environment. The kit contains a specially formulated N-2 Plus Media Supplement, which has been optimized for neural stem cell expansion (Johe, K.K. et al. (1996) Genes & Development 10:3129). Human fibroblast growth factor basic (FGF basic) is included to promote the in vitro proliferation of neural precursors. Human epidermal growth factor (EGF) is also included and may be used for optimizing human neural precursor growth in culture. However, the use of EGF is optional, and its effect, other than stimulating cell growth, is still under investigation. Bovine Fibronectin, used to coat plates to promote cell attachment and spreading, is also included. The quantity of each component provided in the kit is estimated to be sufficient to make 500 mL of growth medium for cell expansion. This kit is suitable for the monolayer culture model and is applicable for human, rat and mouse cells. Cells propagated using this kit are Nestin-positive (Lendahl, U. et al. (1990) Cell 60:585) and retain their potency to differentiate into neurons, oligodendrocytes and glia, as identified after growth factor withdrawal by staining with the antibodies Tuj1, O4 and anti-GFAP, respectively.

Neural stem cells (NSCs), with the potential to self-renew and to differentiate into neurons and glial cells, have been isolated from different regions of the developing and adult brain (1 - 6). NSCs can be grown in vitro, in the presence of mitogen, as either monolayer cultures on an adhesive substrate or as free floating spherical aggregates termed 'neurospheres. These in vitro propagated NSCs can proliferate in culture while retaining the potency to differentiate into neurons, oligodendrocytes and glia. in vitro NSC expansion makes it possible to grow sufficient numbers of NSCs for researchers to study basic developmental biology, drug discovery and preclinical neural transplantation.

  1. Alvarez-Buylla, A. et al. (1998) J. Neurobiol. 36:105.
  2. McKay, R.D. (1997) Science 276:66.
  3. Reynolds, B.A. et al. (1992) Science 255:1707.
  4. Johansson, C.B. et al. (1999) Cell 96:25.
  5. Kilpatrick, T.J. et al. (1993) Neuron 10:255.
  6. Davis, A.A. et al. (1994) Nature 372:263.

Neural Stem Cell Expansion Kits SC002 & SC005 are suitable for a monolayer culture model, and Neural Stem Cell Expansion Kit SC003 is for a neurosphere culture model.

Info R&D SystemsR&D Systems
R&D Systems, Inc.
614 McKinley Place N.E.
Minneapolis, MN 55413
Customer Service: 1 (800) 343-7475
1 (612) 379-2956

Fax Number: 1 (612) 656-4400
Web Site: http://www.rndsystems.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. NeuroCyte - Rat Neural Stem Cell Kit from Orion BioSolutions, Inc.
2. Neural Stem Cell Expansion Kit - Neurosphere from R&D Systems
3. Neural Stem Cell Expansion Kit - Monolayer from R&D Systems
4. Mouse/Rat Neural Stem Cell Functional Identification Kit from R&D Systems
5. Human Neural Stem Cell Functional Identification Kit from R&D Systems
6. rNeural Stem Cell (rNSC)Kit from AlphaGenix, Inc.
7. Expansion and Cryopreservation of Cells from Meridian Life Science, Inc.
8. Stem Cell Expansion Media 1 from 3H Biomedical AB
9. Mouse Hematopoietic Stem Cell Expansion Cytokine Panel from R&D Systems
10. Mouse Dopaminergic Neuron Differentiation Kit, 2nd Generation from R&D Systems
11. Free Activin A ELISA Kit from Diagnostic Systems Laboratories, Inc.
dithiothreitol, sulfhydryl reducing agent, C4H10O2S2; MW: 154.24, 97% pure (minimum), CAS No: 27565-41-9, store at -20 C ...
Human LT beta R/TNFRSF3 Phycoerythrin MAb (Clone 71319)...
Human ROR1 Affinity Purified Polyclonal Ab...
Phospho-p70 S6 Kinase (T229) Affinity Purified PAb...
Biology Products:
(Date:11/17/2016)... Global Market Watch: Primarily supported by ... Banks and Academics) market is to witness a value of ... the highest Compounded Annual Growth Rate (CAGR) of 10.75% is ... analysis period 2014-2020. North America is ... Europe at 9.56% respectively. Report ...
(Date:11/14/2016)... Technology, Inc. ("xG" or the "Company") (Nasdaq: XGTI, XGTIW), ... in challenging operating environments, announced its results for the ... a conference call to discuss these results on November ... Key Recent Accomplishments ... acquire Vislink Communication Systems. The purchase is expected to ...
(Date:6/22/2016)... -- On Monday, the Department of Homeland Security (DHS) ... for the Biometric Exit Program. The Request for Information ... explains that CBP intends to add biometrics to confirm ... States , in order to deter visa overstays, ... Logo - http://photos.prnewswire.com/prnh/20160622/382209LOGO ...
Breaking Biology News(10 mins):
(Date:12/8/2016)... DIEGO, Dec. 8, 2016  OncoSec Medical Incorporated ... developing DNA-based intratumoral cancer immunotherapies, today announced financial ... "We are delivering on our commitment ... with ImmunoPulse┬« IL-12. We are pleased with the ... combination trial, and we are focused on advancing ...
(Date:12/8/2016)... , Dec. 8, 2016 Eurofins announces the appointment of ... President of Eurofins Scientific Inc. (ESI). Mr. Murray ... proven professional and entrepreneurial experience in leading international business teams. As ... food testing market to uphold Eurofins, status as the global leader ... , ...
(Date:12/8/2016)... 8, 2016  Anaconda BioMed S.L., a pre-clinical stage ... next generation neuro-thrombectomy system for the treatment of Acute ... Jovin, MD to join its Scientific Advisory Board (SAB). ... network of scientific and clinical experts to Anaconda BioMed ... ANCD BRAIN ┬« to its clinical phase. The ...
(Date:12/8/2016)... N.J. , Dec. 8, 2016  Soligenix, ... late-stage biopharmaceutical company focused on developing and commercializing ... an unmet medical need, announced today the long-term ... with SGX942 (dusquetide), a first-in-class Innate Defense Regulator ... in head and neck cancer patients undergoing chemoradiation ...
Breaking Biology Technology: