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NDPase (GDPase, UDPase, ADPase) Assay Kit from Sigma-Aldrich

ProductsNDPase (GDPase, UDPase, ADPase) Assay Kit from Sigma-Aldrich
Company Sigma-Aldrich
Item NDPase (GDPase, UDPase, ADPase) Assay Kit
Price 
Description The kit was tested on rat tissue extracts (liver, kidney, spleen, and heart) and on a yeast (S. cerevisae) extract. It was also tested with microsomal fractions prepared from rat tissues and cell lines.
Application: A simple and reliable method for measuring NDPase activity in sub-cellular fractions based on the detection of the inorganic phosphate released from NDP by the appropriate NDPase. The color formation, measured at 700 nm in the presence of molybdate, is proportional to the amount of the inorganic phosphate released by enzymes.
Biochem/physiol Actions: GDPases/UDPases (NDPs) in yeasts are located exclusively in the Golgi complex and are a convenient marker for Golgi detection.
Preparation Unit Definition: 1 unit of NDPase releases 1 μmole of inorganic phosphate per minute at 25 C under conditions specified by the assay.
Info Sigma-AldrichSigma-Aldrich
Sigma-Aldrich Corp.
St. Louis, MO, USA
Customer Service: 800-325-3010
Tech Support: 800-325-5832
Fax Number: 314-771-5757
Web Site: http://www.sigmaaldrich.com/
NOTE: Price information is approximate list price and actual prices may vary.

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... target blotted protein on PVDF membrane or ... The N-terminal sequence of proteins (5-20 residues) ... by HPLC or mono-and bidimensional SDS-PAGE, followed ... using the well known EDMAN degradation method. ...
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