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Mts-Atf-Biotin Label Transfer Reagent, 5 mg from Pierce Biotechnology, Inc.

ProductsMts-Atf-Biotin Label Transfer Reagent, 5 mg from Pierce Biotechnology, Inc.
Company Pierce Biotechnology, Inc.
Item Mts-Atf-Biotin Label Transfer Reagent, 5 mg
Price 
Description Biotin label transfer is fast emerging as a powerful new tool for protein interaction discovery and confirmation. Until now, only amino groups have been able to be targeted with label transfer reagents such as our Sulfo-SBED. Sulfhydryl-reactive Mts-Atf-Biotin and its long chain analog, Mts-Atf-LC-Biotin, are exciting additions to the protein interaction tool chest that offer a ground-floor opportunity to use them to discover or confirm a protein interaction. • The label-transfer method involves transfer of a biotin molecule from a purified bait protein to a binding partner designated as the prey protein. Using these reagents, a -SH-containing bait protein is first biotinylated via the Mts moiety. After removal of excess reagent and incubation in the dark with a putative prey protein or prey protein-containing lysate, the Atf moiety is activated with UV light capturing the interacting complex. Reduction of the disulfide bond formed by reaction of -SH with the Mts group of the label-transfer reagent results in the transfer of the biotin label from the bait protein to the prey protein. The biotin can be used to both enrich for and detect the interacting complex or prey protein using immobilized Monomeric Avidin or Streptavidin-HRP-based detection, respectively

2- {N2-[N6-(4-Azido-2,3,5,6-tetrafluorobenzoyl-6-amino-caproyl)-N6-(6-biotinamidocaproyl)-L-lysinylamido]}ethyl methanethiosultonate • Supplied as a solid and packaged under argon. Store desiccated at -20C protected from light.
Info Pierce Biotechnology, Inc.Pierce Biotechnology, Inc.
Pierce Biotechnology, Inc.
3747 N. Meridian Rd.
P.O. Box 117
Rockford, IL 61105
Customer Service: 815-968-0747
Fax Number: 815-968-8148
Web Site: http://www.piercenet.com/
NOTE: Price information is approximate list price and actual prices may vary.

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