Navigation Links
Mouse Dopaminergic Neuron Differentiation Kit, 2nd Generation from R&D Systems

ProductsMouse Dopaminergic Neuron Differentiation Kit, 2nd Generation from R&D Systems
Company R&D Systems
Item Mouse Dopaminergic Neuron Differentiation Kit, 2nd Generation
Price $1,000.00
Description Reagents for the differentiation of mouse embryonic stem cells to dopaminergic neurons.

Dopamine cell bodies exist in the midbrain, where they are mainly located in the substantia nigra, the ventral tegmental area, and the retrorubral field. These dopaminergic (DA) neurons give rise to extensive forebrain dopamine innervation ( e.g.projections to the caudate nucleus, putamen nucleus accumbens, olfactory tubercle, and several cortical regions and limbic regions, including the amygdala, lateral septum and ventral hippocampus). The basic organization of midbrain dopamine neurons and their projections is consistent across most mammalians. The midbrain dopamine projections to the striatum have been among the most extensively studied catecholamine neurons, in part because the degeneration of DA neurons in the substantia nigra results in Parkinsons disease. Both animal models and clinical trials have suggested that cell replacement therapies may be effective in treating Parkinsons disease. This approach is limited, however, by the availability of a rich, effective source of neural precursors for DA neuron generation.

Embryonic stem (ES) cells, derived from the inner cell mass of pre-implantation embryos, have been recognized as the most pluripotent stem cell population. ES cells give rise to a collection of differentiated cells and tissues, including neurons. Thus, they may serve as a potential source of replacement DA neurons in Parkinsons disease. Two groups have reported methods for generating DA neurons from ES cells, thus demonstrating the feasibility of identifying conditions required for functional DA neuron differentiation of ES cells. Experimental models established from these studies might facilitate the in vitroexamination of various aspects of neural development, in particular the underlying mechanisms controlling cell lineage and fate commitment.

DESIGN OF THE KIT

The Mouse Dopaminergic Neuron Differentiation Kit is a system designed for in vitroneural differentiation of mouse ES cells in a serum-free environment. This kit contains specially formulated ITS and N-2 Plus Media Supplements, which are used to select and enrich neural stem cell populations. Bovine Fibronectin is included to provide proper support for cell attachment and spreading. A growth factor panel, consisting of human fibroblast growth factor (FGF) basic, mouse FGF-8b, and mouse sonic hedgehog amino-terminal peptide (Shh-N), is included for effective DA differentiation. The quantity of each component provided in the kit is estimated to be sufficient for the induction of 3 x 107 ES cells. This kit has been shown to generate an average of 15 plus/minus 5% DA neurons as estimated from double labeling various mouse ES cell lines with antibodies specific to tyrosine hydroxylase and neuron-specific β-III tubulin.

Info R&D SystemsR&D Systems
R&D Systems, Inc.
614 McKinley Place N.E.
Minneapolis, MN 55413
Customer Service: 1 (800) 343-7475
1 (612) 379-2956

Fax Number: 1 (612) 656-4400
Web Site: http://www.rndsystems.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Mouse Anti-Amodiaquine Monoclonal Antibody, Unconjugated, Clone 6D10 from AntibodyShop A/S
2. Mouse Anti-Protein S Monoclonal Antibody, Unconjugated, Clone HYB 232-02 from Abcam
3. Mouse Anti-Mouse Glutathione S-transferase M1 (GSTM1) Monoclonal Antibody, Unconjugated from Lifespan Biosciences
4. Mouse Anti-Human S-100 alpha/beta chain Monoclonal Antibody, Unconjugated, Clone 8B10 from Santa Cruz Biotechnology, Inc.
5. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Testis from OriGene Technologies
6. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Brain (adult) from OriGene Technologies
7. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (12.5 - day) from OriGene Technologies
8. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (19 - day) from OriGene Technologies
9. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Thymus from OriGene Technologies
10. Mouse Anti-Human Complement factor B Monoclonal Antibody, Unconjugated, Clone 9B6 from AntibodyShop A/S
11. Mouse Anti-Human Choriongonadotropin (hCG) Monoclonal Antibody, Unconjugated, Clone 5F10 from AntibodyShop A/S
... Progesterone Receptor (PR) Competitor Assay ... screening novel progesterone receptor binding compounds ... assay utilizes a fusion of glutathione ... of human progesterone receptor [PR-LBD(GST)] and ...
... the proven features of the pET, BacVector ... all of the benefits and versatility of ... The pTriEx vectors have been extensively tested ... all three expression systems. In each case, ...
Rabbit polyclonal to Shigella Immunogen: Mixture of S. boydii, S. flexneri & S. dysenteriae....
... ALDEFLUOR Stem Cell Identification ... comprises a substrate for ... (ALDH) that is highly ... stem cells, making ALDH ...
Biology Products:
(Date:10/17/2019)... , ... October 15, 2019 , ... ... Media for the Microbiological Community”. , Microbiology International currently manufactures ready-to-use culture broth ... also offers pre-poured agar plates & liquid supplements. , This major step underpins ...
(Date:10/17/2019)... ... October 16, 2019 , ... The International Society for ... Baseline® Guide: Water and Steam Systems (Third Edition) . This guide provides in-depth ... systems. Industry professionals can better understand Good Manufacturing Practices (GMPs), to comply with ...
(Date:10/17/2019)... ... October 16, 2019 , ... Advanced Clinical, a clinical ... continues with the opening of new offices in Bucharest and Timisoara, Romania. Advanced ... country’s accelerated growth in clinical trials. , Advanced Clinical’s entrance into Romania ...
Breaking Biology News(10 mins):
(Date:9/24/2019)... ... 24, 2019 , ... Personalized Stem Cells, Inc ... for clinical trial sites and physician investigators. The clinical trial sites are ... , Clinical trial sites are currently located in San Diego and Los Angeles ...
(Date:9/17/2019)... SOMERSET, N.J. (PRWEB) , ... September 17, 2019 ... ... today announced that Julie Delaney, Director, Project and Clinical Supplies Management, Asia Pacific ... at the One Farrer Hotel, Singapore, on Sept. 25-26, 2019. , On Thursday, ...
(Date:9/17/2019)... , ... September 16, 2019 , ... When a lot ... kits that allow for the complete 3D characterization of tissues through the use of ... into an imaging, image analysis and advanced cell culture focused contract research organization and ...
(Date:9/11/2019)... ... September 09, 2019 , ... Suvoda LLC , ... drug supply chain management, today announced its new technology partnership with Veeva ... “The integration between Veeva Vault EDC and our IRT will enable sponsors and ...
Breaking Biology Technology: