Navigation Links
Mouse Anti-Porcine Pyruvate Dehydrogenase (PDH), complex Immunocapture Kit Monoclonal Antibody, Protein G-Agarose Conjugated, Clone 15D3G9C11 from MitoScience LLC

ProductsMouse Anti-Porcine Pyruvate Dehydrogenase (PDH), complex Immunocapture Kit Monoclonal Antibody, Protein G-Agarose Conjugated, Clone 15D3G9C11 from MitoScience LLC
Company MitoScience LLC
Item Mouse Anti-Porcine Pyruvate Dehydrogenase (PDH), complex Immunocapture Kit Monoclonal Antibody, Protein G-Agarose Conjugated, Clone 15D3G9C11
Price $295.00
Description 250 g monoclonal antibody irreversibly crosslinked to protein G-agarose beads which can immunocapture ~13 g of PDH complex from heart mitochondria. Also included are 2 mg of bovine heart mitochondria for control immunocapture. The pyruvate dehydrogenase complex (PDH) immunocapture kit allows isolation of PDH from small amounts of tissue. This facilitates the subsequent analysis of the assembly state, activity and post-translational modification of the complex. Pyruvate dehydrogenase is a key enzyme in energy metabolism. It converts pyruvate that has been transported into the mitochondrion into the 2-carbon molecule acetyl-CoA for subsequent breakdown in the Krebs cycle (citric acid cycle). PDH activity is under tight control through phosphorylation and dephosphorylation of the E1 alpha subunit, reactions which involve a set of specific pyruvate dehydrogenase kinases (PDK1-4) and phosphatases respectively. The immunocapture antibody isolates a functionally active complex and a protocol has been described for measuring this activity while the enzyme is still bound to the capture beads or fixed in a 96 well plate. Tightly bound kinases and phosphatases are retained on the complex through the immunocapture step. Release of the protein and subsequent gel electrophoresis allows characterization of the phosphorylation state, which can then be analyzed in more detail by mass spectrometry. Uses for this PDH immunocapture kit include but are not limited to examining alterations in assembly of the complex in genetic PDH deficiency altered PDH functioning in late-onset diabetes (NIDMM) and the response of the enzyme complex to diet, exercise and sepsis.
Info MitoScience LLCMitoScience LLC
1850 Millrace Drive
Suite 3A
Eugene, Oregon 97403
Customer Service: (800) 910-6486
Fax Number: (541) 284-1801
Web Site: http://www.mitosciences.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Mouse Anti-Amodiaquine Monoclonal Antibody, Unconjugated, Clone 6D10 from AntibodyShop A/S
2. Mouse Anti-Protein S Monoclonal Antibody, Unconjugated, Clone HYB 232-02 from Abcam
3. Mouse Anti-Mouse Glutathione S-transferase M1 (GSTM1) Monoclonal Antibody, Unconjugated from Lifespan Biosciences
4. Mouse Anti-Human S-100 alpha/beta chain Monoclonal Antibody, Unconjugated, Clone 8B10 from Santa Cruz Biotechnology, Inc.
5. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Testis from OriGene Technologies
6. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Brain (adult) from OriGene Technologies
7. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (12.5 - day) from OriGene Technologies
8. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (19 - day) from OriGene Technologies
9. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Thymus from OriGene Technologies
10. Mouse Anti-Human Complement factor B Monoclonal Antibody, Unconjugated, Clone 9B6 from AntibodyShop A/S
11. Mouse Anti-Human Choriongonadotropin (hCG) Monoclonal Antibody, Unconjugated, Clone 5F10 from AntibodyShop A/S
Sterile, Cell culture tested...
... have been many modifications to the original formulas ... solutions still play an important role in tissue,culture. ... pH and osmotic balance in the medium and ... inorganic ions, is as valuable today as when ...
A substrate for all AChE-based EIA kits....
The Synergy HT utilizes a unique dual-optics design combined with monochromator wavelength selection to provide uncompromised performance in absorbance, fluorescence and luminescence reading modes. ...
Biology Products:
(Date:8/22/2014)... Bethesda, MD FASEB MARC (Maximizing Access to Research ... the FASEB Grant Writing Seminar & Practical Exercises Workshop ... in Bethesda, Maryland from August 25-26, 2014. These ... postdoctorates and research scientists from underrepresented groups in the ... This year MARC conferred 11 awards totaling $20,350. ...
(Date:8/22/2014)... important discoveries in basic and clinical research and ... into a complex offensive spanning multiple fronts. , ... lab could help find new and more selective ... that targets a specific enzyme overexpressed in certain ... from brain tumours. , Chemistry professor Christopher Cairo ...
(Date:8/22/2014)... study of captive black rhino reproduction in Europe ... of breeding programmes. , Researchers from Chester Zoo, ... Liverpool carried out a six-year study which encompassed ... Dr Katie Edwards led the research as part ... She says: "Although some black rhinoceros breed well ...
Breaking Biology News(10 mins):New enzyme targets for selective cancer therapies 2New enzyme targets for selective cancer therapies 3Hormone analysis helps identify horny rhinos 2
... March 7, 2013 (Bronx, NY) Researchers at ... been awarded a $12 million grant from the National Institutes ... prevent HIV infection in women. "As we move into ... having its greatest impact on women, particularly in sub-Sahara Africa," ...
... MD FASEB MARC (Maximizing Access to Research Careers) ... 2013 American College of Sports Medicine (ACSM) Northwest Chapter ... awards are meant to promote the entry of underrepresented ... the basic science community and to encourage the participation ...
... Research Council (BBSRC) has launched a bioscience response to ... that threatens our third most common broadleaf tree (after ... has been awarded to gather an in-depth understanding of ... about some ash trees, natural resistance to attack. Computer ...
Cached Biology News:Einstein receives $12 million grant to develop device for preventing HIV infection 2Einstein receives $12 million grant to develop device for preventing HIV infection 3Bioscience to battle ash dieback 2Bioscience to battle ash dieback 3
(Date:8/22/2014)... August 22, 2014 Nerium International, ... is in the spotlight again. Nerium will showcase ... its NeriumFirmTM Body Contouring Cream to Hollywood’s elite ... Creations during the 66th Primetime Emmy® Awards. , ... extract, third-party clinical trials show that NeriumAD night ...
(Date:8/21/2014)... LYNBROOK, N.Y. , Aug. 21, 2014 /PRNewswire/ ... a biopharmaceutical company developing first in class collagenase-based ... histolyticum or CCH) in the U.S. and XIAPEX ... statistically significant results from a randomized, double-blind Phase ... of cellulite, or edematous fibrosclerotic panniculopathy. The results ...
(Date:8/21/2014)... An international research team including DESY scientists has observed ... The team reports in the journal Science ... lattices inside the nanodroplets. It is the first time ... in larger samples of what is known as superfluid ... exceeded our best expectations," says Andrey Vilesov of the ...
(Date:8/21/2014)... His Majesty Willem-Alexander, King of the ... of His Majesty Willem-Alexander, King of the Netherlands, has ... of POET-DSM Advanced Biofuels’ Project LIBERTY cellulosic ethanol plant ... King will take part in the Grand Opening Ceremony ... a.m.-12:20 p.m. and tour the plant. POET-DSM is a ...
Breaking Biology Technology:Nerium International Joins Television’s Biggest Stars at Backstage Creations Giving Suite at the 66th Primetime Emmy Awards 2Nerium International Joins Television’s Biggest Stars at Backstage Creations Giving Suite at the 66th Primetime Emmy Awards 3BioSpecifics Technologies Corp. Announces Positive Data from Phase 2a Study of CCH for Treatment of Cellulite 2BioSpecifics Technologies Corp. Announces Positive Data from Phase 2a Study of CCH for Treatment of Cellulite 3BioSpecifics Technologies Corp. Announces Positive Data from Phase 2a Study of CCH for Treatment of Cellulite 4BioSpecifics Technologies Corp. Announces Positive Data from Phase 2a Study of CCH for Treatment of Cellulite 5Scientists observe quantum vortices in cold helium droplets 2His Majesty Willem-Alexander, King of the Netherlands, to Attend Project LIBERTY opening 2His Majesty Willem-Alexander, King of the Netherlands, to Attend Project LIBERTY opening 3