Navigation Links
Mouse Anti-Porcine Pyruvate Dehydrogenase (PDH), complex Immunocapture Kit Monoclonal Antibody, Protein G-Agarose Conjugated, Clone 15D3G9C11 from MitoScience LLC

ProductsMouse Anti-Porcine Pyruvate Dehydrogenase (PDH), complex Immunocapture Kit Monoclonal Antibody, Protein G-Agarose Conjugated, Clone 15D3G9C11 from MitoScience LLC
Company MitoScience LLC
Item Mouse Anti-Porcine Pyruvate Dehydrogenase (PDH), complex Immunocapture Kit Monoclonal Antibody, Protein G-Agarose Conjugated, Clone 15D3G9C11
Price $295.00
Description 250 g monoclonal antibody irreversibly crosslinked to protein G-agarose beads which can immunocapture ~13 g of PDH complex from heart mitochondria. Also included are 2 mg of bovine heart mitochondria for control immunocapture. The pyruvate dehydrogenase complex (PDH) immunocapture kit allows isolation of PDH from small amounts of tissue. This facilitates the subsequent analysis of the assembly state, activity and post-translational modification of the complex. Pyruvate dehydrogenase is a key enzyme in energy metabolism. It converts pyruvate that has been transported into the mitochondrion into the 2-carbon molecule acetyl-CoA for subsequent breakdown in the Krebs cycle (citric acid cycle). PDH activity is under tight control through phosphorylation and dephosphorylation of the E1 alpha subunit, reactions which involve a set of specific pyruvate dehydrogenase kinases (PDK1-4) and phosphatases respectively. The immunocapture antibody isolates a functionally active complex and a protocol has been described for measuring this activity while the enzyme is still bound to the capture beads or fixed in a 96 well plate. Tightly bound kinases and phosphatases are retained on the complex through the immunocapture step. Release of the protein and subsequent gel electrophoresis allows characterization of the phosphorylation state, which can then be analyzed in more detail by mass spectrometry. Uses for this PDH immunocapture kit include but are not limited to examining alterations in assembly of the complex in genetic PDH deficiency altered PDH functioning in late-onset diabetes (NIDMM) and the response of the enzyme complex to diet, exercise and sepsis.
Info MitoScience LLCMitoScience LLC
1850 Millrace Drive
Suite 3A
Eugene, Oregon 97403
Customer Service: (800) 910-6486
Fax Number: (541) 284-1801
Web Site: http://www.mitosciences.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Mouse Anti-Amodiaquine Monoclonal Antibody, Unconjugated, Clone 6D10 from AntibodyShop A/S
2. Mouse Anti-Protein S Monoclonal Antibody, Unconjugated, Clone HYB 232-02 from Abcam
3. Mouse Anti-Mouse Glutathione S-transferase M1 (GSTM1) Monoclonal Antibody, Unconjugated from Lifespan Biosciences
4. Mouse Anti-Human S-100 alpha/beta chain Monoclonal Antibody, Unconjugated, Clone 8B10 from Santa Cruz Biotechnology, Inc.
5. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Testis from OriGene Technologies
6. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Brain (adult) from OriGene Technologies
7. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (12.5 - day) from OriGene Technologies
8. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (19 - day) from OriGene Technologies
9. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Thymus from OriGene Technologies
10. Mouse Anti-Human Complement factor B Monoclonal Antibody, Unconjugated, Clone 9B6 from AntibodyShop A/S
11. Mouse Anti-Human Choriongonadotropin (hCG) Monoclonal Antibody, Unconjugated, Clone 5F10 from AntibodyShop A/S
PP2B-Abeta (C-20)...
Goat polyclonal to Duffy / FY / DARC...
... Source: Tritirachium album Description: Proteinase ... used in a wide range of applications purified ... bonds mainly following the carboxyl group of N-substituted ... and is classified as a serine protease. Proteinase ...
... fully licensed, high throughput, water bath thermal cycler ... 24 microplates. This equates to 9,216 reactions per ... arm rapidly transfers a basket of plates between ... that are considerably faster than those of peltier ...
Biology Products:
(Date:7/24/2014)... specialist at The University of Texas Health Science Center ... grant to help Harris County residents whose vision problems ... Even with corrective lenses, many people with low vision ... variety of low vision devices, such as telescopes, magnifiers ... , Iyer is using the three-year, $164,645 SightFirst grant ...
(Date:7/24/2014)... gathered at NJIT this week for an all-day public ... Environmental Protection Agency (EPA) to dredge toxic sediment from ... The EPA has called the plan, which proposes removing ... sections of the riverbed, one of the largest cleanups ... dredged and disposed off-site. , "We need to ensure ...
(Date:7/24/2014)... Calif. -- To reduce fire hazard in the United ... mechanically cutting woody shrubs and suppressed trees (ladder fuels). ... burned during periods of low fire danger in order ... and minimize hazardous air pollutants, managers often cover all ... plastic, commonly referred to as agricultural plastic, in order ...
Breaking Biology News(10 mins):UTHealth Dr. Bhavani Iyer awarded low vision grant 2Experts Weigh the Pros and Cons of a $1.7 Billion EPA Cleanup Plan for the Passaic River at an NJIT 2Study gives new perspective on agricultural plastic, debris burning, and air quality 2
... importance of the world,s natural assets is now firmly on ... showcasing the enormous economic value of forests, freshwater, soils and ... of their loss, was the conclusion of The Economics of ... leader, Pavan Sukhdev. "TEEB has documented not ...
... they have solved an interesting problem: how to get ... complete vegetable protein. What,s more, they,ve joined forces with ... snack they,ve developed into the hands of 1.2 million ... country has decidedly vegetarian tastes, the Indian people just ...
... 19, 2010In response to a study regarding fish oil ... of the Journal of the American Medical Association (JAMA), ... association for the dietary supplement industry, reminds pregnant and ... recommended amounts of docosahexaenoic acid (DHA) throughout pregnancy. This ...
Cached Biology News:TEEB report puts world's natural assets on the global political radar 2TEEB report puts world's natural assets on the global political radar 3TEEB report puts world's natural assets on the global political radar 4TEEB report puts world's natural assets on the global political radar 5TEEB report puts world's natural assets on the global political radar 6Illinois scientists promote soy by currying favor with Indian taste buds 2CRN responds to JAMA fish oil study 2CRN responds to JAMA fish oil study 3
(Date:7/25/2014)... Pa. , July 25, 2014 /PRNewswire-iReach/ -- ... and consulting for global corporations operating in highly ... host its first annual Global User Group Conference, ... historic Philadelphia, Pennsylvania at ... - http://photos.prnewswire.com/prnh/20140724/130263 This ...
(Date:7/24/2014)... 2014 -- A novel combination of microscopy and data ... Oak Ridge National Laboratory an unprecedented look at the ... and electrochemical properties. , The research team led ... surface of a perovskite manganite, a complex material that ... to understand surface behavior could benefit researchers who are ...
(Date:7/24/2014)... BURLINGAME, Calif. , July 24, 2014  ECC ... Denver, Colorado with University College Hospital ... of a development project to finance, design, construct, and ... Ibadan, Nigeria . Under the first ... and prepare scoping documents and funding applications. UCH Ibadan ...
(Date:7/24/2014)... Engineering at the University of Delaware, is known ... energy engineering, environmental sustainability and electronics. , His ... with a well-defined, crystalline structure. At the atomic ... that zeolites can separate molecules with size differences ... of a nanometer), making them useful to the ...
Breaking Biology Technology:Xybion Announces It Will Host International User Conference in Philadelphia 2Xybion Announces It Will Host International User Conference in Philadelphia 3ORNL study reveals new characteristics of complex oxide surfaces 2ECC Signs a Contract for the Finance, Design, and Construction of a Premier Cancer Institute in Ibadan, Nigeria 2University of Delaware researcher describes new approach for creating organic zeolites 2