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MitoPT Detection of Mitochondrial Permeability from B-Bridge International

ProductsMitoPT Detection of Mitochondrial Permeability from B-Bridge International
Company B-Bridge International
Item MitoPT Detection of Mitochondrial Permeability
Description The MitoPT kit is used in conjunction with your existing apoptosis protocols. Grow your cells in culture and induce apoptosis according to your existing procedure (also reserve a non-induced population of cells as a negative control). Once you have induced apoptosis in your cells, add the 1X MitoPT solution to each population and incubate the cells for an additional 15 minutes. During this incubation time, the MitoPT reagent will enter each cell and the mitochondria inside. If the cell is not undergoing apoptosis, the mitochondrial DY will be intact, and the MitoPT reagent will aggregate inside the mitochondria and fluoresce red. If the cell is apoptotic, the mitochondrial DY will be breaking down, thereby causing the MitoPT reagent to be dispersed throughout the entire cell and fluoresce green.

The structure of the MitoPT reagent allows it to easily penetrate cells and healthy mitochondria. Once inside a healthy non-apoptotic cell, the lipophilic MitoPTreagent, bearing a delocalized positive charge, enters the negatively charged mitochondria where it aggregates and fluoresces red (7). These aggregates, first described by Jelley in 1937, are referred to as J-aggregates (8). When the mitochondrial DY collapses in apoptotic cells, the MitoPT reagent no longer accumulates inside the mitochondria. Instead, it is distributed throughout the cell. When dispersed in this manner, the MitoPT reagent assumes a monomeric form, which fluoresces green (9). Use of the MitoPT kit allows the easy distinction between non-apoptotic red fluorescent cells and apoptotic green fluorescent cells.

Info B-Bridge InternationalB-Bridge International
320 Logue Avenue
Mountain View, CA 94043

Customer Service: 650-969-7727
Fax Number: 650-969-7737
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NOTE: Price information is approximate list price and actual prices may vary.

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