Navigation Links
MitoPT Detection of Mitochondrial Permeability from B-Bridge International

ProductsMitoPT Detection of Mitochondrial Permeability from B-Bridge International
Company B-Bridge International
Item MitoPT Detection of Mitochondrial Permeability
Price 
Description The MitoPT kit is used in conjunction with your existing apoptosis protocols. Grow your cells in culture and induce apoptosis according to your existing procedure (also reserve a non-induced population of cells as a negative control). Once you have induced apoptosis in your cells, add the 1X MitoPT solution to each population and incubate the cells for an additional 15 minutes. During this incubation time, the MitoPT reagent will enter each cell and the mitochondria inside. If the cell is not undergoing apoptosis, the mitochondrial DY will be intact, and the MitoPT reagent will aggregate inside the mitochondria and fluoresce red. If the cell is apoptotic, the mitochondrial DY will be breaking down, thereby causing the MitoPT reagent to be dispersed throughout the entire cell and fluoresce green.

The structure of the MitoPT reagent allows it to easily penetrate cells and healthy mitochondria. Once inside a healthy non-apoptotic cell, the lipophilic MitoPTreagent, bearing a delocalized positive charge, enters the negatively charged mitochondria where it aggregates and fluoresces red (7). These aggregates, first described by Jelley in 1937, are referred to as J-aggregates (8). When the mitochondrial DY collapses in apoptotic cells, the MitoPT reagent no longer accumulates inside the mitochondria. Instead, it is distributed throughout the cell. When dispersed in this manner, the MitoPT reagent assumes a monomeric form, which fluoresces green (9). Use of the MitoPT kit allows the easy distinction between non-apoptotic red fluorescent cells and apoptotic green fluorescent cells.

Info B-Bridge InternationalB-Bridge International
320 Logue Avenue
Mountain View, CA 94043

Customer Service: 650-969-7727
Fax Number: 650-969-7737
Web Site: http://www.b-bridge.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. MitoPT-25:MITOCHONDRIAL PERMEABILITY TRANSITION KIT from AbD Serotec
2. Synergy HT Multi-Detection Microplate Reader from BioTek Instruments
3. Alkaline Phosphatase Detection Kit from CHEMICON
4. miRCURY LNA in situ Detection probes from Exiqon
5. miRCURY LNA Northern Blotting Detection probes from Exiqon
6. PARP Cleavage Detection Kit from Calbiochem
7. APO LOGIX - JC-1 Mitochondrial Membrane Potential Detection Kit from BACHEM
8. Product Information Request - SuperSignal West Pico COMPLETE Biotinylated Protein Detection Kit from Pierce Biotechnology, Inc.
9. DNA Engine Opticon Continuous Fluorescence Detection System from Bio-Rad
10. MyiQ Single-Color Real-Time PCR Detection System from Bio-Rad
11. MyiQ Single-Color Real-Time PCR Detection System from Bio-Rad
(Date:5/23/2017)... robotic gym for the rehabilitation and functional motor sense evaluation of lower ... . The first 30 robots will be available from June in ... The technology was developed and patented at the IIT laboratories and has ... to a 10 million euro investment from entrepreneur Sergio Dompè. ... ...
(Date:5/6/2017)... 2017 RAM Group , Singaporean ... breakthrough in biometric authentication based on a ... to perform biometric authentication. These new sensors are based ... by Ram Group and its partners. This sensor will ... chains and security. Ram Group is a next ...
(Date:4/18/2017)... 18, 2017  Socionext Inc., a global expert in SoC-based imaging ... server, the M820, which features the company,s hybrid codec technology. A ... Tera Probe, Inc., will be showcased during the upcoming Medtec Japan ... at the Las Vegas Convention Center April ... Click here for ...
Breaking Biology News(10 mins):
(Date:6/16/2017)... ... 2017 , ... Cognition Corporation , a software company ... of its “From the Helm” Webinar Series. , The next two free ... design control exercises. Led by David Cronin, Cognition’s CEO, the half-hour public webinars ...
(Date:6/16/2017)... (PRWEB) , ... June 16, 2017 , ... CTNext ... Entrepreneur Innovation Awards (EIA), held at The LOFT at Chelsea Piers in Stamford. , ... ideas to a panel of judges for an opportunity to secure $10,000 awards to ...
(Date:6/15/2017)... ... June 15, 2017 , ... ... secured a Series B round of financing in the amount of $6 million. ... private investors participated in the round. , The Series B funding will enable ...
(Date:6/15/2017)... , ... June 15, 2017 , ... ... a promising new medical device startup. Dan Parsley, angelMD’s SVP of Corporate Development, ... members, and this angelMD syndicate is part of Saranas’ recently announced $4 million ...
Breaking Biology Technology: