Navigation Links
Mast Cell Degranulation colorimetric Assay Kit from CHEMICON

ProductsMast Cell Degranulation colorimetric Assay Kit from CHEMICON
Company CHEMICON
Item Mast Cell Degranulation colorimetric Assay Kit
Price $375.00
Description Degranulation, the secretion of cytoplasmic granules, is a key step in the inflammatory response of leukocytes (e.g. basophils, neutrophils, eosinophils, and mast cells). In addition to histamine, these secretory granules contain many proinflammatory mediators including heparin, cytokines, chemokines, and many proteases (Hallgren, 2001). Tryptase, a tetrameric serine proteinase, has emerged as the major component of mast cell granules, comprising up to 20% of the total protein of mast cells derived from lung, colon and skin tissue (He, 2004; He, 1998; Schwartz, 1986). Because it is stored almost exclusively in mast cells, tryptase is a popular indicator of mast cell activation and a target for therapeutic intervention in allergic diseases. The Chemicon Mast Cell Degranulation Assay Kit provides a quick, efficient and sensitive system for evaluation of tryptase activity in cell lysates, supernatants or for inhibitor screening.

Test Principle

Chemicons Mast Cell Degranulation Assay Kit provides a simple and convenient means for assaying tryptase activity. The assay is based on spectophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate tosyl-gly-pro-lys-pNA. The free pNA can then be quantified using a spectrophotometer or a microtiter plate reader at 405 nm. A pNA Standard is provided as a comparison control.

Calcium Ionophore A23187 (Calcimycin) is included in the kit for degranulation induction of samples. Calcimycin has been a well-documented inducer of tryptase release from human mast cells (2,3).

A tryptase inhibitor, protamine, is included as a test inhibitor for screening purposes. Protamine is a potent heparin antagonist that competitively binds heparin, an essential stabilizing factor of mast cell tryptase (Hallgren, 2001; Schwartz, 1986). Protamine has been shown to inhibit both human and mouse tryptase (Hallgren, 2001). The kits Assay Buffer contains heparin for tryptase stabilization.

Info CHEMICONCHEMICON
CHEMICON International, Inc.
28820 Single Oak Drive
Temecula, CA 92590
Customer Service: 800-437-7500
Tech Support: 800-437-7500
Fax Number: 800-437-7502
Web Site: http://www.chemicon.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. XpressPack™ Colorimetric Amplification Detection System, required for colorimetric XpressPack™ from CHEMICON
2. Glutathione-S-Transferase (GST) Colorimetric Assay Kit from Sigma-Aldrich
3. In Vitro Osteogenesis Assay Kit from CHEMICON
4. PR Competitor Assay Green from Invitrogen
5. PolarScreen Androgen Receptor Competitor Assay Kit, Red from Molecular Probes (Invitrogen)
6. Hydrogen Peroxide / Peroxidase Fluorometric (resorufin) Assay Kit, Fluoro H2O2 from BACHEM
7. PPARGamma-Competitor Assay Kit Green from Invitrogen
8. Total antioxidant capacity Colorimetric Assay Kit from Neogen Corporation
9. PolarScreen Progesterone Receptor Competitor Assay Kit, Far Red from Molecular Probes (Invitrogen)
10. Total Antioxidant Power Colorimetric Assay Kit from Neogen Corporation
11. NDPase (GDPase, UDPase, ADPase) Assay Kit from Sigma-Aldrich
... PowerPac HV high-voltage power supply, with temperature probe, ... of 5,000 V, 500 mA, and 400 W. ... temperature between 0 and 90 degrees C during ... transfer. Power cord and instructions are provided. Dimensions ...
... 111 mini IEF cell is an apparatus ... using polyacrylamide or agarose slab gels. The ... graphite electrodes, casting tray, 5 glass plates, ... polyacrylamide, 5 sample templates, and instructions. Dimensions ...
... Biomek FX provides complete solutions ... With 96-, 384-, and ... in either single or dual ... offers maximum speed and flexibility ...
BD Falcon, microtest ELISA plate 96 well flat-bottom, clear. Polystyrene, nonsterile, no lid (25/sp, 100/ca) Packaging: 25 / pack, 100 / case ...
Biology Products:
(Date:4/24/2017)... -- Janice Kephart , former 9/11 Commission ... LLP (IdSP) , today issues the following statement: ... 6, 2017 Executive Order: Protecting the Nation ... instilled with greater confidence, enabling the reactivation of ... are suspended by until at least July 2017). ...
(Date:4/19/2017)... 19, 2017 The global military ... is marked by the presence of several large global ... by five major players - 3M Cogent, NEC Corporation, ... for nearly 61% of the global military biometric market ... the global military biometrics market boast global presence, which ...
(Date:4/18/2017)... Inc., a global expert in SoC-based imaging and computing solutions, has ... features the company,s hybrid codec technology. A demonstration utilizing TeraFaces ® ... be showcased during the upcoming Medtec Japan at Tokyo Big Sight ... Las Vegas Convention Center April 24-27. ... Click here for an image of the ...
Breaking Biology News(10 mins):
(Date:4/27/2017)... California (PRWEB) , ... April 27, 2017 , ... ... new infographic explaining why mass flow controllers based on capillary thermal mass flow ... industrial gas mass flow control applications. Over 80% of all industrial processes—such ...
(Date:4/27/2017)... ... 27, 2017 , ... The Council for Agricultural Science and ... Lusk, a consummate communicator who promotes agricultural science and technology in the public ... explains how innovation and growth in agriculture are critical for food security and ...
(Date:4/26/2017)... SAN DIEGO, CALIF. (PRWEB) , ... April 26, ... ... Mother’s Day? Lajollacooks4u, San Diego’s premiere team-building and cooking events company, offers ... cooking classes. , Menus specialize in California cuisine, and guests leave inspired ...
(Date:4/26/2017)... Centennial, CO (PRWEB) , ... April 25, 2017 , ... ... test offerings were added to its extensive test menu: Nucleic Acid Testing (NAT) ... that time, LABS was only able to offer NAT screening for blood donors under ...
Breaking Biology Technology: