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MagneGST™ Protein Purification System from Promega

ProductsMagneGST™ Protein Purification System from Promega
Company Promega
Item MagneGST™ Protein Purification System
Price Inquire
Description The MagneGST™ Protein Purification System provides a simple, rapid and reliable method for the purification of glutathione-S-transferase (GST) fusion proteins. Immobilized glutathione paramagnetic particles (MagneGST™ Particles) are used to isolate GST-tagged protein directly from a crude or cleared lysate using either a manual (requires use of a magnetic stand) or automated procedure. GST-tagged proteins can be purified on a small scale from 1ml of culture or on a large scale using more than 50ml of culture. Samples can also be processed using a robotic platform.

Features:
One-step purification of multiple samples with easy handling. • After cell lysis, no requirement for high-speed centrifugation to clear lysate. • Scalable protocol using 1 to 50ml of cell culture. • Achieve high yields with little or no nonspecific background. • Protocols available for both manual and automated procedures. • System contains all necessary components except magnetic stand. • Automated protocols for this system are available online • Compatible with Many Robotic Platforms: Validated automated methods available for Beckman Coulter Biomek® 2000.

Applications:
Protein purification of glutathione-S-transferase fusion proteins from cleared or crude E. coli cell lysate.
Info PromegaPromega
2800 Woods Hollow Rd.
Madison, WI 53711-5399 USA
Customer Service: 800-356-9526
Tech Support: 1-800-356-9526
Fax Number: 800-356-1970
Web Site: http://www.promega.com
NOTE: Price information is approximate list price and actual prices may vary.

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... balance makes your life easier. It offers a wide ... for one balance. *Instruments supported: ... UMX, AG, XS, XP, PR, SR, PG-S, PG, SG, ... User guidance on ...
... Purification Kits enable rapid analytical to medium ... produced from appropriate vectors. Sufficient reagents are ... mg target protein under native or denaturing ... Agarose, which specifically retains STag fusion proteins. ...
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... (g/L):,Tryptone, 10 ,Yeast extract, 5 ,Sodium ... ,Ferric ammonium citrate, 0.5 ,IPTG, 0.03 ... soluble, autoclavable chromogenic substrate for -galactosidase. ... -galactosidase generates a black insoluble precipitate. ...
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