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Luciferase Reporter Assay Kit from MBL International

ProductsLuciferase Reporter Assay Kit from MBL International
Company MBL International
Item Luciferase Reporter Assay Kit
Price 
Description c-Jun N-terminal kinase (JNK) is one of the several main MAP kinase groups Firefly luciferase has been used as a sensitive reporter for studying gene regulation and function. Luciferase provides a 1000-fold increase in sensitivity in comparison to the standard chloramphenicol acetyltransferase (CAT) assay. Firefly luciferase catalyzes the oxidative carboxylation of luciferin, a reaction with the highest efficiency of any known bioluminescence reaction. The light emission from the reaction can be recorded using a luminometer. MBLs Luciferase Reporter Assay Kit provides a simple means for detecting luciferase activity in transfected eukaryotic cells. For measurement of expressed luciferase in vitro, luciferase is first extracted from transfected cells through cell lysis. Then CoA, ATP, Mg2+ and buffer are added to the lysate (Inclusion of CoA yields a nearly constant light emission rather than typical flash Kinetics). The luminescent reaction is then triggered by an injection of luciferin, and the emitted light is recorded by a luminometer. By providing CoA, along with ATP, Mg2+ in an optimized buffer solution, MBLs Luciferase Reporter Assay Kit ensures maximal sensitivity and consistent light output, as well as convenience and consistency when working with multiple samples.
Info MBL InternationalMBL International
15 B Constitution Way
Woburn, MA 01801
Customer Service: 781-939-6964
Fax Number: 781-939-6963
Web Site: http://mblintl.com
NOTE: Price information is approximate list price and actual prices may vary.

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