Navigation Links
Luciferase Luminescent Assay Kit, LucLite from PerkinElmer

ProductsLuciferase Luminescent Assay Kit, LucLite from PerkinElmer
Company PerkinElmer
Item Luciferase Luminescent Assay Kit, LucLite
Description Measurement of light production by luciferase-cDNA transfected cell lines has proven to be a valuable technique in the development of reporter gene assays in which the luciferase is used as an indicator for the transcription of other genes. The choice of the luciferase enzyme is due to advantages such as high sensitivity and relatively simple measurement. With traditional assay systems , signal measurement must be completed within a limited time period due to the short half-life of the reaction (half-life approximately five minutes). This means only one plate at a time can be prepared and counted.

The PerkinElmer luclite Constant Quanta assay system produces a long lived glow type signal with a half-life of several hours, which makes it ideal for use on the PerkinElmer LumiCount Microplate Luminometer or the PerkinElmer TopCount Microplate Scintillation and Luminescence Counter. When performing high-throughput assays using luclite and the TopCount, you can prepare thousands of samples in microplates, load them into the microplate stacker, and analyze all samples unattended. The luclite assay system also provides the most simple procedure available - just mix two vials of reagent, dispense into the wells of a microplate containing transfected cells, seal the plate, shake briefly and measure.

The signal obtained with luclite has a linear decay characteristic after an initiation phase of approximately one minute. Luclites long-lived luminescent signal produced is cell type dependent, but is generally stable for up to five hours. There are no additional cell lysis steps necessary when using luclite.

Kit Contents:
10 x 50 mL of substrate solution (lyophilized), 2 x 250 of buffer solution, Instruction booklet.
Info PerkinElmerPerkinElmer
PerkinElmer Life & Analytical Sciences.
710 Bridgeport Avenue
Shelton, CT 06484-4794, USA

Call PerkinElmer to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-866-823-5510
Customer Service: (800) 762-4000; Outside the USA: (+1) 203-925-4602
Fax Number: (203) 944-4904
Web Site:
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. pMIR-mU6-Luc, Mouse U6 Luciferase Control Vector from Mirus Bio Corporation
2. pMIR-hU6-Luc, Human U6 Luciferase Control Vector from Mirus Bio Corporation
3. Luciferase Reporter Assay Kit from MBL International
4. Luciferase Control RNA from Promega
5. The LAS-1000 plus Luminescent Image Analysis System from FUJIFILM Life Science
6. XpressPack™ Luminescent Amplification Detection System, required for luminescent XpressPack™ from CHEMICON
7. Glutathione-S-Transferase (GST) Colorimetric Assay Kit from Sigma-Aldrich
8. In Vitro Osteogenesis Assay Kit from CHEMICON
9. Mast Cell Degranulation colorimetric Assay Kit from CHEMICON
10. PR Competitor Assay Green from Invitrogen
11. PolarScreen Androgen Receptor Competitor Assay Kit, Red from Molecular Probes (Invitrogen)
... Design with Purpose -- ... MS provide the highest level ... performance in an affordable, easy-to-use ... autosampler and workstation guarantees success ...
One-step, microplate or cuvet, colorimetric, detection range 0.1 mg/dL to 3 mg/dL. Procedure: 10 min....
... RNA Ladder is suitable for sizing single-stranded RNA ranging ... formaldehyde agarose gels. The ladder consists of nine bands ... 6 8 and 10 kb) to simplify size estimation. ... other bands allowing easy orientation and identification of RNA ...
... Serum • Mouse serum is collected off the clot ... weeks of age. Available Anticoagulants: N-02: ... K2EDTA N-08: Potassium Oxalate N-10: ... ACD N-07: CPD N-09: ...
Biology Products:
(Date:6/2/2016)... 2016 Perimeter Surveillance & Detection ... Physical Infrastructure, Support & Other Service  The ... offers comprehensive analysis of the global Border Security ... revenues of $17.98 billion in 2016. Now: ... leader in software and hardware technologies for advanced video ...
(Date:5/20/2016)... , May 20, 2016  VoiceIt is excited ... with VoicePass. By working together, VoiceIt ...  Because VoiceIt and VoicePass take slightly different approaches ... increases both security and usability. ... about this new partnership. "This marketing ...
(Date:5/9/2016)... Elevay is currently known as ... for high net worth professionals seeking travel for work ... world, there is still no substitute for a face-to-face ... your deal with a firm handshake. This is why ... of citizenship via investment programs like those offered by ...
Breaking Biology News(10 mins):
(Date:6/23/2016)... Houston Methodist Willowbrook Hospital has signed ... to serve as their official health care provider. ... will provide sponsorship support, athletic training services, and ... volunteers, athletes and families. "We are ... and to bring Houston Methodist quality services and ...
(Date:6/23/2016)... , June 23, 2016   EpiBiome , a ... $1 million in debt financing from Silicon Valley Bank ... automation and to advance its drug development efforts, as ... facility. "SVB has been an incredible strategic ... services a traditional bank would provide," said Dr. ...
(Date:6/23/2016)... , June 23, 2016 Apellis Pharmaceuticals, ... 1 clinical trials of its complement C3 inhibitor, ... and multiple ascending dose studies designed to assess ... of subcutaneous injection in healthy adult volunteers. ... either as a single dose (ranging from 45 ...
(Date:6/23/2016)... ... June 23, 2016 , ... ClinCapture, the only free ... and will showcase its product’s latest features from June 26 to June 30, ... poster on Disrupting Clinical Trials in The Cloud during the conference. DIA ...
Breaking Biology Technology: